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Anti ecadherin rat antibody

Manufactured by Cell Signaling Technology

The Anti-E-cadherin rat antibody is a laboratory reagent used to detect and study the expression of the E-cadherin protein in various experimental systems. E-cadherin is a cell-cell adhesion molecule that plays a crucial role in maintaining the integrity of epithelial tissues. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunocytochemistry to visualize and analyze the presence and distribution of E-cadherin in biological samples.

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2 protocols using anti ecadherin rat antibody

1

Immunofluorescence Staining of Cell Markers

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The following primary and secondary antibodies were used for immunofluorescence: anti-Ecadherin rat antibody (Cell Signaling Technology, #3195, 1:100 dilution), anti-Myosin-VIIa rabbit polyclonal antibody (Proteus BioSciences Inc., #25-6790, 1:200 dilution), anti-Histone H3 (phospho S28) rat polyclonal antibody (Abcam, #ab10543, 1:200 dilution), Alexa Fluor 546-conjugated goat anti-rat IgG (H+L) antibody (Thermo Fisher Scientific, #A11081, 1:1000 dilution), Alexa Fluor 647-conjugated goat anti-rabbit IgG (H+L) antibody (Abcam, #ab150079, or Thermo Fisher Scientific, #A21247, 1:1000 dilution).
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2

Immunofluorescence Imaging of E-cadherin and SOX9

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The following primary and secondary antibodies were used for immunofluorescence: anti-Ecadherin rat antibody (#3195, 1:100 dilution, Cell Signaling Technology), anti-SOX9 rabbit antibody (#AB5535-25UG, 1:200 dilution, Merck Millipore), Alexa Fluor 546-conjugated goat anti-rat IgG (H+L) antibody (#A11081, 1:1000 dilution), Alexa Fluor 647-conjugated goat anti-rat IgG (H+L) antibody (#A21247, 1:1000 dilution), Alexa Fluor 647-conjugated goat anti-rabbit IgG (H+L) antibody (#A32733, 1:1000 dilution)(all Thermo Fisher Scientific).
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