Tenax ta

The leaf volatiles were subjected to gas chromatography-mass spectrometry (GC-MS) on an Agilent system consisting of a model 7890 gas chromatograph, a model 5977 mass-selective detector (EIMS, electron energy of 70 eV), and an Agilent ChemStation data system (Santa Clara, CA, USA). Volatiles in the leaf were trapped into an odorant-collecting cartridge (Tenax TA, GL Sciences, Tokyo, Japan) and subjected to GC using a newly developed non-solvent method known as dynamic-headspace and thermal-desorption system (Gerstel, Überhausen, Germany) with COMPS2XLxt multi-purpose sampler. The GC column was a DB-VRX column (Agilent, USA) with a lm thickness of 1.44 µm, length of 60 m, and internal diameter of 0.25 mm. The carrier gas was helium, with a ow rate of 2.1 mL/min. The GC oven temperature was regulated as follows: 40ºC initial temperature held for 3 min; increased at 5ºC/min to 260ºC and held for 8 min. Leaf samples (0.2 g) were placed into 20 mL vials and measured by the dynamic-headspace technique. Mass-selective detector was set at 230ºC. The volatiles were identi ed by comparing their MS fragmentation patterns to those in the MS library (NIST14 database).

The leaf volatiles were subjected to gas chromatography-mass spectrometry (GC-MS) on an Agilent system consisting of a model 7890 gas chromatograph, a model 5977 mass-selective detector (EIMS, electron energy of 70 eV), and an Agilent ChemStation data system (Santa Clara, CA, USA). Volatiles in the leaf were trapped into an odorant-collecting cartridge (Tenax TA, GL Sciences, Tokyo, Japan) and subjected to GC using a newly developed non-solvent method known as dynamic-headspace and thermal-desorption system (Gerstel, Überhausen, Germany) with COMPS2XLxt multi-purpose sampler. The GC column was a DB-VRX column (Agilent, USA) with a lm thickness of 1.44 µm, length of 60 m, and internal diameter of 0.25 mm. The carrier gas was helium, with a ow rate of 2.1 mL/min. The GC oven temperature was regulated as follows: 40ºC initial temperature held for 3 min; increased at 5ºC/min to 260ºC and held for 8 min. Leaf samples (0.2 g) were placed into 20 mL vials and measured by the dynamic-headspace technique. Mass-selective detector was set at 230ºC. The volatiles were identi ed by comparing their MS fragmentation patterns to those in the MS library (NIST14 database).