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Ab133524

Manufactured by Novus Biologicals
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Ab133524 is a laboratory antibody product. It is a purified polyclonal antibody that can be used for research purposes.

Automatically generated - may contain errors

Lab products found in correlation

Ab124954, by Abcam (2 mentions)

2 protocols using ab133524

1

Quantitative Immunoblotting Analysis

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Immunoblotting was performed as described before. 22 Following antibodies and dilutions were used: Trx: 1:5000 from abcam/ab133524, TXNIP: 1:1000 from Novus Biologicals/clone JY2 and TXNRD: 1:10 000 from Abcam/ ab124954. Bands were quantified by densitometry with ImageJ Version 1.46r.
Ahrens T.D., Timme S., Ostendorp J., Bogatyreva L., Hoeppner J., Hopt U.T., Hauschke D., Werner M, & Lassmann S. (2016). Response of esophageal cancer cells to epigenetic inhibitors is mediated via altered thioredoxin activity. Laboratory investigation; a journal of technical methods and pathology, 96(3).
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2

Immunohistochemical Analysis of Esophageal Trx, TXNIP, and TXNRD

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Immediate serial sections (3 μm) of human tissue specimens were subjected to deparaffinization, antigen retrieval and immunohistochemical staining with appropiate antibodies (Trx: 1:5000 from abcam/ab133524; TXNIP: 1:5000 from Novus Biologicals/clone JY2; TXNRD: 1:800 from Abcam/ ab124954). IHC was evaluated for nuclear as well as cytoplasmic positivity in normal esophageal epithelial cells and invasive tumor cells with the following scores: score 0 = negative (o10% positive cells), score 1 = weak and/or heterogeneous protein expression (from 10 to 70% positive cells), and score 2 = strong expression and homogeneous in 470% cells. For TXNIP and TXNRD, only basal layers were considered for scoring in normal esophageal epithelia.
Ahrens T.D., Timme S., Ostendorp J., Bogatyreva L., Hoeppner J., Hopt U.T., Hauschke D., Werner M, & Lassmann S. (2016). Response of esophageal cancer cells to epigenetic inhibitors is mediated via altered thioredoxin activity. Laboratory investigation; a journal of technical methods and pathology, 96(3).
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