Asolectin

Manufactured by Avanti Polar Lipids

Sourced in United States

Asolectin is a natural phospholipid mixture derived from soybean. It is composed primarily of phosphatidylcholine, phosphatidylethanolamine, and other related phospholipids. Asolectin serves as a source of phospholipids for various applications in research and industry.

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Lab products found in correlation

Deoxycholate, by Merck Group (1 mentions) Dodecyl maltoside, by Anatrace (1 mentions) Docosahexaenoic acid dha, by Cayman Chemical (1 mentions) Cholesterol, by Merck Group (1 mentions) Retinoic acid, by Merck Group (1 mentions)

3 protocols using asolectin

Planar Lipid Bilayer Formation and Cyt c Interaction

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Asolectin (Avanti Polar Lipids, Alabaster, AL, USA) was used for the formation of planar lipid bilayer membranes (BLMs). Bulk solutions containing 0.1 M KCl and 5 mM Tris-HCl buffer (all reagents were of analytical grade) were used, and pH values were set to 6.4, 7.4, and 8.4. Cyt c from a bovine heart (Merck KGaA, Darmstadt, Germany) water solution of various concentrations (initial concentration being 5 mg/mL) was added to the bulk solutions.

Anosov A., Borisova E., Smirnova E., Korepanova E, & Osipov A. (2023). Effect of Cytochrome C on the Conductance of Asolectin Membranes and the Occurrence of Through Pores at Different pHs. Membranes, 13(3), 268.

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Lipid Composition Analysis Protocol

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Anapoe20 (Tween20) and dodecyl maltoside (lauryl maltoside; DDM) were purchased from Anatrace (Maumee, OH). Docosahexaenoic acid (DHA) was purchased from Cayman Chemicals (Ann Arbor, MI). Cholesterol, deoxycholate (DOC), and retinoic acid (RA) were purchased from Sigma. Asolectin (soybean polar lipids) was reported by the supplier (Avanti Polar Lipids, Alabaster, AL) to contain 45.7% phosphatidylcholine, 22.1% phosphatidylethanolamine, 18.4% phosphatidylinositol, 6.9% phosphatidic acid, and 6.9% unspecified phospholipids and pigments as determined by HPLC/ELSD.

Hiser C., Liu J, & Ferguson-Miller S. (2018). The K-path entrance in cytochrome c oxidase is defined by mutation of E101 and controlled by an adjacent ligand binding domain. Biochimica et biophysica acta, 1859(9), 725-733.

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Proton Pumping Ability of Cytochrome c Oxidase

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To investigate proton pumping ability purified CytcO was reconstituted into small unilamellar vesicles made of soybean polar lipid extract (asolectin) obtained from Avanti Polar Lipids (product number 541602) [38 (link)]. A cholate dialysis method [38 (link)] was used for the reconstitution. Before usage, the asolectin was precipitated with acetone and then extracted with diethyl ether. The cholate was removed by dialysis as previously described [39 (link)].

Berg J., Liu J., Svahn E., Ferguson-Miller S, & Brzezinski P. (2019). Structural changes at the surface of cytochrome c oxidase alter the proton-pumping stoichiometry. Biochimica et biophysica acta. Bioenergetics, 1861(2), 148116.

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