On the 54th day after disease induction, blood specimens were collected from orbital sinuses of the mice. All of the procedures were performed according to the approved protocols for animals. Red blood cell (RBC) count and hemoglobin (Hb) levels were measured for each sample with the Sysmex automated blood analyzer.
Automated blood analyzer
Manufactured by Sysmex
Sourced in Japan
The Automated Blood Analyzer is a laboratory instrument designed to automatically analyze and measure various components of a blood sample. It provides accurate and reliable data on the composition of the blood, including red blood cells, white blood cells, platelets, and other key parameters. The device utilizes advanced technologies to perform these analyses efficiently and with minimal human intervention.
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Lab products found in correlation
4 protocols using automated blood analyzer
1
Blood Analysis of Disease-Induced Mice
2
Poultry Serum Lipid and Antibody Analysis
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On d 17 and d 35, 2 chicks were randomly selected from each pen, and blood samples were collected from brachial vein using 5 mL syringe (BD, Franklin Lakes, NJ, USA), and then were immediately centrifuged at 1,500×g for 15 min at 4°C. Each serum after centrifugation was stored at −20°C until use. Lipid layers of blood were analyzed for content comparison of triglyceride, total-cholesterol, high-density lipoprotein cholesterol, glutamic oxaloacetic transaminase (GOT), and glutamic pyruvic transaminase (GPT) using automated blood analyzer (Sysmex, Seoul, Korea). Immunoglobulins (Ig)A, IgG, and IgM antibodies were detected using enzyme-linked immunosorbent assay kits (BETHYL, Montgomery, TX, USA). Absorbance for detection was determined at 470 nm using a microplate reader (Bio-Tek, Winooski, VT, USA).
3
Organ and Blood Biomarkers in Chickens
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Two birds per replicate (eight birds per treatment) were selected and weighed individually. The liver, spleen, cecum, and abdominal fat were evaluated using samples obtained from these chickens at the end of the experiment. Organ weight was expressed as g/100 g body weight. Blood samples were collected from the brachial vein in 5-mL vacutainer tubes. Serum was obtained through centrifugation at 2,000×g for 15 min and stored at −70°C until analysis. Serum was analyzed to compare the levels of albumin, globulin, total cholesterol, glutamic oxaloacetic transaminase (GOT), glutamic-pyruvic transaminase (GPT), and blood urea nitrogen (BUN) using an automated blood analyzer (Sysmex, Seoul, Korea).
4
Hematologic and Biochemical Profiling in Rats
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In week 10 and 18 of the study and after rats’ fasting for 12 hrs, blood samples were collected and the hematologic parameters were assayed using automated blood analyzer (Sysmex, Japan) to determine red blood cell (RBC) count, hemoglobin (Hb), hematocrit (Hct) and mean cell volume (MCV) levels. The plasma of blood samples was separated using refrigerated micro centrifuge (Cleaver Scientific, England). They were then used for biochemical assay of glucose, creatinine (Cr), blood urea nitrogen (BUN), uric acid, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) by commercial ELISA kits (Pars Azmoon, Tehran, Iran), and then microplate reader (Bioteck, USA). Insulin was assayed by ELISA kits (Zellbio, Ulm, Germany) and homeostasis model assessment of insulin resistance (HOMA-IR) index was computed based on fasting plasma insulin and glucose levels according to HOMA-IR=(fasting insulin (µg/L) × fasting blood glucose (mg/dL)/405)22 (link),23 (link) formula.
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