Fluostar galaxy 2300

In vivo permeability assay to assess epithelial barrier function was performed using FITC-D as described [76 (link)]. Briefly, food was withdrawn for 4 h from 5-to-6-weeks-old C3H/HeN mice in various groups and gavaged with an 80 mg/100 g body weight of FITC-D, (molecular weight 4000; Sigma–Aldrich). Serum was collected at the time of euthanasia, blood cells were removed by centrifugation, and the fluorescence intensity of each sample was measured with a fluorimeter (excitation, 492 nm; emission, 525 nm; FLUOstar Galaxy 2300; BMG Labtech, Durham, NC, USA). FITC-D concentrations were determined from standard curves generated by the serial dilution of FITC-D, and permeability was calculated by linear regression of sample fluorescence (Excel 5.0; Microsoft).

In-vivo permeability assay to assess epithelial barrier function was performed using FITC-D as described [34 (link)]. Briefly, food was withdrawn for 4 h from 5- to 6-weeks-old NIH: Swiss mice in various groups and gavaged with 80mg/100g body weight of FITC-D, (molecular weight 4,000; Sigma-Aldrich). Serum was collected at the time of euthanasia and the fluorescence intensity of each sample was measured with a fluorimeter (excitation, 492 nm; emission, 525 nm; FLUOstar Galaxy 2300; BMG Labtech, Durham, NC). FITC-D concentrations were determined from standard curves generated by serial dilution of FITC-D and permeability was calculated by linear regression of sample fluorescence (Excel 5.0; Microsoft).