1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), cholesterol and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt) (Rh-DPPE) were obtained from Avanti Polar Lipids. All lipid bilayers used in fluorescence imaging were doped with 0.1 mol% Rh-DPPE. Giant unilamellar vesicles were formed via electro-formation in 300 mM sucrose solution, using standard protocols. [25] [26] [27] Around 0.5-2 µL of GUV solution was pipetted onto the PDMS substrate, previously wetted with 500 µL trizma buffer (10 mM trizma base, 150 mM NaCl, 2 mM CaCl 2 ). Within two minutes, the vesicles fused to the PDMS substrates (both partially and fully oxidized) and formed membrane patches. The remaining unfused vesicles were gently washed away with 500 µL trizma buffer.
1 2 dipalmitoyl sn glycero 3 phosphocholine dppc cholesterol
Manufactured by Avanti Polar Lipids
Sourced in United States
1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and cholesterol are chemical compounds commonly used in laboratory research. DPPC is a type of phospholipid, while cholesterol is a sterol molecule. These compounds are often utilized in the study of lipid bilayer membranes and their properties.
Automatically generated - may contain errors
Lab products found in correlation
1 2 dioleoyl sn glycero 3 phosphocholine dopc, by Avanti Polar Lipids (1 mentions)
1 2 dipalmitoyl sn glycero 3 phosphoethanolamine n lissamine rhodamine b sulfonyl ammonium salt rh dppe, by Avanti Polar Lipids (1 mentions)
Oasis hlb 1 cc vac cartridge, by Waters Corporation (1 mentions)
D luciferin, by PerkinElmer (1 mentions)
Olaparib, by Selleck Chemicals (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
α cyano 4 hydroxycinnamic acid, by Merck Group (1 mentions)
Trypsin edta solution, by Thermo Fisher Scientific (1 mentions)
1 2 dioleoyl 3 trimethylammonium propane chloride salt dotap, by Avanti Polar Lipids (1 mentions)
1 2 distearoyl sn glycero 3 phosphoethanolamine n amino polyethyleneglycol 2000 dspe peg 2000 amine, by Avanti Polar Lipids (1 mentions)
Sodium cholate hydrate, by Merck Group (1 mentions)
Dspe peg2k, by Lipoid (1 mentions)
Cpg odn 2395, by InvivoGen (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Sodium citrate, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Tert butanol, by Merck Group (1 mentions)
1 2 dioleoyl 3 trimethylammonium propane chloride salt, by Avanti Polar Lipids (1 mentions)
4 protocols using 1 2 dipalmitoyl sn glycero 3 phosphocholine dppc cholesterol
1
Supported Bilayer Formation from GUVs
2
Nanoparticle-Mediated Olaparib Delivery
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1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), cholesterol, 1,2-distearoyl-sn-glycero-3 phosphoethanolamine-N-[amino(polyethyleneglycol)-2000] (DSPE-PEG-2000 Amine), and 1,2-dioleoyl-3-tri methyl-ammonium-propane (chloride salt) (DOTAP) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Olaparib was purchased from Selleck Chemicals (Houston, TX, USA). 2-Hydroxyl-propyl-β-cyclodextrine and α-cyano-4-hydroxycinnamic acid were purchased from Sigma -Aldrich Co. (St Louis, MO, USA). All solvents used were HPLC grade from Sigma -Aldrich Co. DMEM, PBS, trypsin–EDTA solution, and FBS were purchased from Gibco (Gaithersburg, MD, USA). d -Luciferin was purchased from PerkinElmer Inc. (Waltham, MA, USA). Cisplatin was purchased from Patterson Veterinary Supply Inc. (Devens, MA, USA) as a 1 mg/mL saline solution (volume 50 mL). Oasis HLB 1 cc Vac Cartridges were purchased from Waters (Milford, MA, USA).
3
Formulation and Characterization of Lipid Nanoparticles
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The
lipids 1,2-dipalmitoyl-sn-glycero-3-phosphocholine
(DPPC), cholesterol, 1,2-dioleoyl-3-trimethylammonium-propane
(chloride salt) (DOTAP), and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (ammonium salt) (DOPE-PEG2k)
were supplied from Avanti Polar Lipids. The lipophilic carbocyanine
dye 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine
perchlorate (DiI) was supplied from Thermo Fisher Scientific. The
CpG ODN 2395 was supplied from Invivogen. The stealth liposome mix
(consisting of hydrogenated soyl -α-phosphatidylcholine
(HSPC), cholesterol, and DSPE-PEG2k in a weight ratio of 3:1:1) was
acquired from Lipoid GmbH (Germany). Reagents for the buffers used
for preparation of the NPs (phosphate-buffered saline (PBS), sodium
chloride, sodium citrate, and Hepes, glucose, and sodium cholate hydrate),
99.9% absolute ethanol, and tert-Butanol were all
supplied from Sigma-Aldrich (Denmark).
lipids 1,2-dipalmitoyl-sn-glycero-3-phosphocholine
(DPPC), cholesterol, 1,2-dioleoyl-3-trimethylammonium-propane
(chloride salt) (DOTAP), and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (ammonium salt) (DOPE-PEG2k)
were supplied from Avanti Polar Lipids. The lipophilic carbocyanine
dye 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine
perchlorate (DiI) was supplied from Thermo Fisher Scientific. The
CpG ODN 2395 was supplied from Invivogen. The stealth liposome mix
(consisting of hydrogenated soy
(HSPC), cholesterol, and DSPE-PEG2k in a weight ratio of 3:1:1) was
acquired from Lipoid GmbH (Germany). Reagents for the buffers used
for preparation of the NPs (phosphate-buffered saline (PBS), sodium
chloride, sodium citrate, and Hepes, glucose, and sodium cholate hydrate),
99.9% absolute ethanol, and tert-Butanol were all
supplied from Sigma-Aldrich (Denmark).
4
Lipid-based Antioxidant and Cytokine Assay Protocol
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Highly purified lipids such as 1,2-dipalmitoyl-Sn-glycero-3-phosphocholine (DPPC), cholesterol were bought from the Avanti Polar Lipids (Alabaster, AL, USA). Cytokines and antioxidants estimation kits were obtained from the Abcam (Cambridge, UK). Whereas Ellagic acid, Cyclophosphamide and RPMI were from MedChem Express (Monmouth Junction, NJ, USA). Nutrient Agar and Tryptic soya broth (TSB) were procured from the HiMedia Company (Mumbai, India).
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