Skov3 ddp

All staple strands, including those with linker extensions, were purchased from Sangon Biotech (Shanghai, China), purified via high affinity purification (HAP), and diluted to a final concentration of 100 μM in 1 × TAE (Tris, Acetic acid, EDTA) buffer with 12.5 mM Mg²⁺. The FAM (Fluorescein maleimide)-modified DNAs were purified by high performance liquid chromatography (HPLC). M13mp18 was obtained from New England Biolabs (NEB, Massachusetts, USA). Ultrafiltration centrifugal tubes were purchased from Millipore Sigma (Burlington, MA, USA). Human ovarian cancer cell lines (SKOV3/DDP, A2780, A2780/DDP, COC1) and a normal ovarian epithelial cell line (IOSE80) were purchased from the China Center for Type Culture Collection (CCTCC, Wuhan, China). Fetal bovine serum (FBS) and RPMI 1640 medium were bought from Gibco (Shanghai, China). Total RNA was extracted using TRIzol (Solarbio, Beijing, China), chloroform, and isopropanol. RNase-free water was used in all RNA-related experiments. A miRNA Reverse Transcription Kit and 2 × SYBR Green qPCR master mix were supplied by Sangon Biotech.

Cisplatin-resistant SKOV3/DDP and parental SKOV3 cells were purchased from China Center for Type Culture Collection and preserved at the Key Laboratory of Molecular Center of Jiangxi Province. The cells were routinely cultured in RPMI-1640 medium containing fetal bovine serum (FBS; 10%), penicillin/streptomycin (100 U/ml) in a 5% humidified CO₂ atmosphere at 37°C. Cisplatin (0.3 µg/ml) was added to the SKOV3/DDP culture media to maintain its acquired resistance.