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3 protocols using candida albicans

1

Antimicrobial Activity of B. pilosa Leaves

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Antimicrobial activity of methanolic leaves extract of B. pilosa was checked by the agar well diffusion and broth micro dilution methods. Pathogens used for the study were gram positive bacteria Staphylococcus aureus (MTCC-96); Bacillus subtilis (MTCC-2097) and Micrococcus luteus (MTCC-2070); gram negative bacteria Escherichia coli (MTCC-739); Pseudomonas aeruginosa (MTCC-2453) and a yeast pathogen Candida albicans (MTCC-3017), obtained from microbial type culture collection (MTCC), Chandigarh, India.
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2

Antibacterial and Antifungal Efficacy Evaluation

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Analytical grade silver nitrate (AgNO3, 99% pure), tetracycline, kanamycin, nutrient agar, potato dextrose agar, Mueller–Hinton agar used in the present study are purchased from Himedia Lab, Ltd., Mumbai, India. The endophytic bacteria Pantoea anthophila (GenBank accession no. MN077163) identified earlier by 16S ribosomal RNA gene sequencing from W. indica were pure cultured and stored in the Department of Biotechnology, Vinayaka Mission's Kirupananda Variyar Engineering College, Salem. Bacterial pathogens such as Gram-positive Staphylococcus epidermidis (MTCC737), Bacillus subtilis (MTCC1133), Staphylococcus aureus (MTCC2940), Gram-negative Escherichia coli (MTCC40), Proteus mirabilis (MTCC425), Salmonella typhi (MTCC733), Klebsiella pneumoniae (MTCC2405) and fungal strains of Aspergillus niger (MTCC404), Candida albicans (MTCC183) and Penicillium chrysogenum (MTCC947) were obtained from Microbial Type Culture Collection (MTCC), Chandigarh.
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3

Microbial Pathogen Culture Collection and Characterization

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All the test microbial pathogens such as Staphylococcus aureus (MTCC 7443), Bacillus subtilis (MTCC 121), Bacillus cereus (MTCC 430), Staphylococcus epidermidis (MTCC 435), Escherichia coli (MTCC 7410), Klebsiella pneumonia (MTCC 7407), Salmonella typhi (MTCC 733), Shigella flexneri (MTCC 1457), Vibrio parahaemolyticus ((MTCC 451), Xanthomonas campestris (MTCC 7908), and Candida albicans (MTCC 183) were procured from Microbial Type Culture Collection and Gene Bank (MTCC; Chandigarh, India).
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