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Axio imager 2 compound microscope

Manufactured by Zeiss

The Axio Imager 2 is a compound microscope designed for a variety of microscopy applications. It features high-quality optics, a sturdy and ergonomic design, and advanced illumination options to support observation and imaging of samples.

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2 protocols using axio imager 2 compound microscope

1

Amber Fossil Specimen Preparation

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All specimens (except FXBA10101) are housed at Nanjing Institute of Geology and Palaeontology, Chinese Academy of Sciences; FXBA10101 is housed in the Lingpoge Amber Museum in Shanghai. The amber has been polished with sand papers with different grain sizes and diatomite mud. Photomicrographs were taken using the Zeiss Discovery V20 microscope system, and those with green background (Figs 2d–f and 3a,b; Supplementary Figs 2d,e, 5a,c,d, 6a,c and 8) were using fluorescence as light source attached to a Zeiss Axio Imager 2 compound microscope. Finally, a compression fossil (NIGP153699) was examined with a LEO1530VP field emission scanning electron microscope.
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2

Multicolor in situ hybridization in zebrafish

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Single and two-color ISH were done as described previously (Lekven et al., 2001 (link); Oxtoby and Jowett, 1993 (link)). All probes were reported previously: tcf21 (ZDB-GENE-051113-88), myod (ZDB-GENE-980526-561), dlx2a (ZDB-GENE-980526-212), elnb (ZDB-GENE-061212-2), fgf3 (ZDB-GENE-980526-178), fgf8a (ZDB-GENE-990415-72), bmp2b (ZDB-GENE-980526-474), bmp4 (ZDB-GENE-980528-2059), and zsyellow (accession number: Q9U6Y4). INT-BCIP (Roche) and Fast red (Sigma) were used for two-color ISH. For images, embryo yolks were manually removed, the embryos were flat mounted and imaged using a Zeiss AxioImager2 compound microscope.
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