Oligo dt 18 or random hexamer primers

Manufactured by Thermo Fisher Scientific

Oligo-dT(18) or random-hexamer primers are short synthetic oligonucleotides used in reverse transcription reactions to generate complementary DNA (cDNA) from RNA templates. Oligo-dT(18) primers bind to the poly(A) tail of mRNA, while random-hexamer primers can bind to any region of the RNA molecule, enabling the synthesis of cDNA from both messenger and non-polyadenylated RNAs.

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Lab products found in correlation

Superscript 3 reverse transcriptase, by Thermo Fisher Scientific (2 mentions) Turbo dnase 1, by Thermo Fisher Scientific (2 mentions) Direct zol rna miniprep kit, by Zymo Research (1 mentions) Random hexamer primer, by Thermo Fisher Scientific (1 mentions) Direct zoltm rna miniprep kit, by Zymo Research (1 mentions)

Spelling variants of this product

Random hexamers (967 citations) Oligo-dT primers (948 citations) Random primers (937 citations) Random hexamer primers (705 citations) Oligo dT (457 citations) Oligo(dT)18 Primer (174 citations) Oligo(dT)18 (51 citations) Oligo-dT random primers (3 citations) Hexamer random primers (2 citations) Oligo‐hexamers (2 citations)

2 protocols using oligo dt 18 or random hexamer primers

Total RNA Extraction and Analysis

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Total RNA for AtPRP40 mRNAs, pri-miRNA, and miRNA level analyses was prepared according to Dolata et al. (2021) . Briefly, RNA was isolated using a Direct-zol RNA Mini Prep Kit (Zymo Research) and treated with Turbo DNase I (Thermo Fisher Scientific).
For transcript expression levels, 3 µg of total DNase-treated RNA was reverse transcribed to cDNA with the use of SuperScript III Reverse Transcriptase (Thermo Fisher Scientific) and oligo-dT(18) or random-hexamer primers (Thermo Fisher Scientific).

Stepien A., Dolata J., Gulanicz T., Bielewicz D., Bajczyk M., Smolinski D.J., Szweykowska-Kulinska Z, & Jarmolowski A. (2022). Chromatin-associated microprocessor assembly is regulated by the U1 snRNP auxiliary protein PRP40. The Plant Cell, 34(12), 4920-4935.

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Transcriptomic Analysis of Arabidopsis

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Total RNA for AtPRP40 mRNAs, pri-miRNA and miRNA level analyses was prepared according to 56 . Briefly, RNA was isolated using a Direct-zol TM RNA Mini Prep Kit (Zymo Research) and treated with Turbo DNase I (Thermo Fisher Scientific).
For transcript expression levels, 3 µg of total DNase-treated RNA was reverse transcribed to cDNA with the use of SuperScript III Reverse Transcriptase (Thermo Fisher Scientific) and oligo-dT (18) or random-hexamer primers (Thermo Fisher Scientific).
Nascent transcripts and chromatin-associated RNAs were separated from the nucleoplasm using the protocol described by 57 . cDNA was prepared with the use of random-hexamer primers (Thermo Fisher Scientific).

Stępień A., Dolata J., Gulanicz T., Bielewicz D., Bajczyk M., Smoliński D.J., Szweykowska-Kulińska Z., & Jarmołowski A. (2022). Chromatin-associated microprocessor assembly is regulated by PRP40, the U1 snRNP auxiliary protein.

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