TIRF microscopy experiments were performed on a home-built system based on a Zeiss Axiovert 200 microscope equipped with a 100×NA=1.46 Plan-Apochromat objective (Zeiss). Samples were illuminated stroboscopically using a 488 nm OPSL laser (Coherent) for excitation of mGFP-GPI, or a 642 nm diode laser (TOPTICA) for excitation of the Atto647N Fab or Cholesterol-KK114. After appropriate filtering, the fluorescence signal was recorded on an Andor IXON Ultra EMCCD camera.
Na 1.46 plan apochromat objective
Manufactured by Zeiss
The 100×NA=1.46 Plan-Apochromat objective from ZEISS is a high-performance microscope objective designed for advanced imaging applications. It provides a numerical aperture of 1.46, enabling the capture of high-resolution images with excellent contrast and detail. The objective is optimized for plan-apochromatic correction, ensuring consistent image quality across the entire field of view.
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Lab products found in correlation
2 protocols using na 1.46 plan apochromat objective
1
TIRF Microscopy of Fluorescent Proteins
2
TIRF Microscopy of Fluorescent Proteins
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