Total protein were extracted and subjected to Western blotting as previous described [23 ]. Monoclonal rabbit primary antibodies against E-cadherin, β-catenin, vimentin, Src and p-Src (Tyr416) (Cell Signal Technology, Boston, MA, USA), polyclonal rabbit primary antibody against Flot2 (Abcam, San Francisco, CA, USA), TGF-β RI (Santa Cruz, Dallas, TX, USA) and the secondary fluorescence goat anti-rabbit antibody (LI-COR, Lincoln, NE, USA) were used in this experiment. The blots were scanned using Odyssey imaging system (LI-COR).
Secondary fluorescence goat anti rabbit antibody
Manufactured by LI COR
Sourced in United States
The Secondary fluorescence goat anti-rabbit antibody is a laboratory reagent used in various immunoassay techniques. It functions as a detection antibody, binding to primary antibodies raised against rabbit antigens, and emitting a fluorescent signal that can be measured and quantified.
Automatically generated - may contain errors
2 protocols using secondary fluorescence goat anti rabbit antibody
1
Probing Epithelial-Mesenchymal Transition Markers
2
Western Blotting for Protein Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total proteins were extracted and subjected to Western blot as described previously[8 (link)]. The polyclonal rabbit primary antibodies against MACC1 (Abcam, Cambridge, Massachusetts, USA), TWIST1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), VEGF-A antibodies (Proteintech, Chicago, IL, USA), and the secondary fluorescence goat anti-rabbit antibody (LI-COR, Lincoln, NE, USA) were used in this experiment. The blots were scanned using Odyssey imaging system (LI-COR).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!