Cells were washed with phosphate-buffered saline (PBS) and lysed with sodium dodecyl sulfate (SDS) lysis buffer [60 mM Tris-HCl, pH 6.8, 1 % SDS in distilled water (DW)] containing protease inhibitor cocktail (Roche, Mannheim, Germany). Equal amounts of protein from each sample were separated by SDS-polyacrylamide gel electrophoresis on 8%–12% gels and transferred onto polyvinylidene difluoride membranes (Millipore, Billerica, MA, USA). Membranes were then incubated with primary antibodies against monocarboyxlate transporters: MCT1 (AB3538P; Millipore), MCT2 and MCT4 (SC50322 and SC50329; Santa Cruz Biotechnology, Santa Cruz, CA, USA), acetyl Co-A synthase 2 (SC85258; Santa Cruz Biotechnology), fatty acid synthase (3189; Cell Signaling Technology, Danvers, MA, USA), and actin (A1978; Sigma-Aldrich, St. Louis, MO, USA). Membranes were washed in PBS and incubated with goat anti-rabbit (sc2004) or anti-mouse (sc2005) IgG horseradish peroxidase (Santa Cruz Biotechnology) as the secondary antibody. Labeled, specific protein bands were visualized using the ECL Kit (Thermo Scientific, Waltham, MA, USA).
Anti mouse sc2005 igg horseradish peroxidase
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-mouse (sc2005) IgG horseradish peroxidase is a secondary antibody conjugated with horseradish peroxidase. It is designed for the detection of mouse primary antibodies in various immunodetection techniques.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti mouse sc2005 igg horseradish peroxidase
1
Western Blot Analysis of Metabolic Proteins
2
Western Blot Analysis of Signaling Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were washed with PBS and lysed with 1 % sodium dodecyl sulfate (SDS) lysis buffer (60 mM Tris–HCl, pH 6.8, 1 % SDS) with protease inhibitor cocktail (Roche, Mannheim, Germany). Equal amounts of total protein from each sample were separated by SDS-polyacrylamide gel electrophoresis on 8 % or 12 % gels, and transferred onto polyvinylidene difluoride membranes (Millipore, Billerica MA, USA). Following incubation with primary antibodies against mammalian target of rapamycin (mTOR; 2972), phospho-mTOR (2448), protein kinase B (AKT; 9272), phospho-AKT (9271), AMP-activated protein kinase (AMPK; 2603), phospho-AMPK (2535), cell cycle-regulation antibody (9932, 9870; Cell Signaling Technology, Danvers, MA), p62 (SC28359; Santa Cruz Biotechnology, Dallas, Texas, USA), LC3B (L7543), and actin (A1978; Sigma), membranes were incubated with goat anti-rabbit (sc2004) or anti-mouse (sc2005) IgG horseradish peroxidase (Santa Cruz Biotechnology) as the secondary antibody. Labeled, specific protein bands were visualized using the ECL Kit (Thermo Scientific, Rockford, IL, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!