Anti cd4 antibodies clone gk1

Manufactured by BioXCell

Sourced in United States

Anti-CD4 antibodies (clone GK1.5) are a laboratory research tool used to recognize and bind to the CD4 cell surface receptor. They are commonly used in flow cytometry and other immunological assays to identify and study CD4+ T cells.

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Lab products found in correlation

Anti asialo gm1 antiserum, by Fujifilm (1 mentions) Anti cd8α clone 2, by BioXCell (1 mentions) Rat igg2b isotype control clone ltf 2, by BioXCell (1 mentions)

2 protocols using anti cd4 antibodies clone gk1

Immune Cell Depletion Protocol

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Mice were treated weekly with 200 µg anti-CD4 antibodies (clone GK1.5, Bio X Cell, NH, USA), 200 µg anti-CD8α (clone 2.43, Bio X Cell), 10 µL anti-asialo GM1 antiserum (Wako Pure Chemical Industries, Osaka, Japan), or rat IgG2b isotype control (clone LT F-2, Bio X Cell) by intraperitoneal injection 3 days prior to irradiation. CD8⁺, CD4⁺, T cells, and NK cell depletion were confirmed by flow cytometry.

Yoon Y.N., Lee E., Kwon Y.J., Gim J.A., Kim T.J, & Kim J.S. (2022). PI3Kδ/γ inhibitor BR101801 extrinsically potentiates effector CD8+ T cell-dependent antitumor immunity and abscopal effect after local irradiation. Journal for Immunotherapy of Cancer, 10(3), e003762.

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Immune Cell Depletion in Tumor Models

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In experiments involving the depletion of CD8⁺ or CD4⁺ cells, mice received intravenous injections of either 50μg of anti-CD8β antibodies (clone 53–5.8, BioXcell) or 50μg of anti-CD4 antibodies (clone GK1.5, BioXcell) on a weekly basis. The efficiency of depletion was evaluated by assessing peripheral blood samples 48 h after the initial depletion. Tumor growth was assessed using the method described above.

Josi R., Speiser D.E., de Brot S., Vogt A.C., Sevick-Muraca E.M., Tolstonog G.V., Bachmann M.F, & Mohsen M.O. (2024). A tetravalent nanovaccine that inhibits growth of HPV-associated head and neck carcinoma via dendritic and T cell activation. iScience, 27(4), 109439.

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