All bioluminescence imaging (BLI) was performed using an Ami HTX imager from Spectral Instruments Imaging (Tuscon, AZ). Animals were anesthetized in a chamber with 3.5% isoflurane, and then 150 mg/kg D-luciferin (VivoGlo, Promega, Madison, WI) was injected intraperitoneally. Mice were then placed into the imager while maintaining anesthesia and an image was obtained five minutes after D-luciferin injection. Exposure time was five minutes per image. AMIView Imaging Software, version 1.5.0 was used to analyze the region of interest and measure photon flux (photons/sec/cm2/sr). Images were obtained on ex vivo necropsy tissues shortly after necropsy with an additional five-minute exposure time.
Ami htx imager
Manufactured by Spectral Instruments Imaging
Sourced in Azerbaijan
The Ami HTX imager is a multi-modal imaging system designed for scientific applications. It provides high-resolution, quantitative imaging capabilities across a range of modalities, including fluorescence, luminescence, and absorbance. The core function of the Ami HTX is to capture and analyze images of samples, enabling researchers to study a variety of biological and material science phenomena.
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Lab products found in correlation
2 protocols using ami htx imager
1
Bioluminescence Imaging in Mice
2
Bioluminescent Tumor Imaging in Mice
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Metastatic tumor formation and colonization were monitored weekly using an AMI HTX imager (Spectral Instruments Imaging, Tuscon AZ). Mice were anesthetized with 2.5% isoflurane in a chamber, moved to nose cone and maintained anesthesia at 2.5% isoflurane during imaging. Mice were injected intraperitoneally with 200 µl of 15 mg/ml D-luciferin substrate, incubated 5 min then dorsal and ventral images were taken.
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