Polystyrene flow cytometry tubes

Manufactured by BD

Sourced in United States

Polystyrene flow cytometry tubes are designed for use in flow cytometry applications. They are made of polystyrene, a common plastic material, and are intended to hold samples during the flow cytometry process.

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Lab products found in correlation

Facscalibur flow cytometer, by BD (2 mentions) Propidium iodide, by Merck Group (2 mentions) Fcs express software, by De Novo Software (1 mentions) Lsrfortessa, by BD (1 mentions) Rpmi 1640 medium, by Lonza (1 mentions) Facscanto, by BD (1 mentions) Lsrfortessa hts flow cytometer, by BD (1 mentions)

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Flow cytometry (2933 citations) Polystyrene tubes (26 citations) Flow cytometry tubes (20 citations) Flow tube (9 citations) Cytometry tubes (2 citations)

5 protocols using polystyrene flow cytometry tubes

Flow Cytometry Analysis of Erythrocytes and GMVs

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For erythrocytes, 4–8 million cells were added per 200 µL per round-bottom sample tube and either treated first with membrane-perturbing enzymes (as described above) or with BODIPY-labeled MUS:OT 2:1 amph-AuNPs at 0.07, 0.14, or 0.28 mg/mL for 1 hr. Tubes were rotated continuously at a desired incubation temperature. Samples from tubes were transferred to 96-well round-bottom plates (BD) or polystyrene flow cytometry tubes (BD Falcon) for analysis. Samples were analyzed with a LSR Fortessa HTS flow cytometer (BD) and data were analyzed using FlowJo software.
For GMVs, flow cytometry samples were prepared in 96-well round-bottom plates (BD) or polystyrene flow cytometry tubes (BD Falcon). We first prepared solutions of 50 mM sucrose in water with BODIPY-MUS:OT 2:1 amph-AuNPs at 0.07 mg/mL in sample wells. GMV harvests in sucrose were mixed 1–2x with a pipette and added at a 1:1.5 ratio per sample well. Samples were rocked gently back and forth after preparation – but there was no additional mixing of GMVs with AuNPs in the wells. Samples were incubated at 1–3 hr and 22°C, unless otherwise noted. Samples were then analyzed with a LSR Fortessa HTS flow cytometer (BD) and data were analyzed using FlowJo software.

Atukorale P.U., Yang Y.S., Bekdemir A., Carney R.P., Silva P.J., Watson N., Stellacci F, & Irvine D.J. (2015). Influence of the Glycocalyx and Plasma Membrane Composition on Amphiphilic Gold Nanoparticle Association with Erythrocytes. Nanoscale, 7(26), 11420-11432.

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Assessing ASC Oligomerization in PBMCs

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Intracellular ASC oligomer formation was evaluated by seeding an individual's PBMC samples from responders, nonresponders, and healthy donors in polystyrene flow cytometry tubes (Falcon) with Opti-MEM Reduced-Serum Media. PBMCs from responders and nonresponders were analyzed at baseline before treatment and after 6 months of fingolimod treatment. After PBMC stimulation, cells were stained for the detection of an ASC oligomer by time-of-flight inflammasome evaluation^{14 (link),15 (link)} using the phycoerythrin-conjugated mouse monoclonal anti-ASC antibody (clone HASC-71, catalog 653903, BioLegend, 1:500). Monocytes were gated using the fluorescein isothiocyanate-conjugated mouse monoclonal anti-CD14 antibody (clone M5E2, catalog 557153, BD Biosciences, 1:10) and using the phycoerythrin-Cy7–conjugated mouse monoclonal anti-CD16 antibody (clone 3G8, catalog 557744, BD Biosciences, 1:10). In all cases, samples were analyzed by flow cytometry using fluorescence-activated cell sorting Canto (BD Biosciences) and the flow cytometry standard express software (De Novo Software).

Malhotra S., Hurtado-Navarro L., Pappolla A., Villar L.M., Río J., Montalban X., Pelegrin P, & Comabella M. (2023). Increased NLRP3 Inflammasome Activation and Pyroptosis in Patients With Multiple Sclerosis With Fingolimod Treatment Failure. Neurology® Neuroimmunology & Neuroinflammation, 10(3), e200100.

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Intracellular ASC-speck Analysis in Monocytes

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Intracellular ASC-speck formation in human monocytes was evaluated by seeding 50 µl of individuals’ whole blood samples in polystyrene flow cytometry tubes (Falcon) with RPMI 1640 medium (Lonza) containing 10% FCS and 2 mM Glutamax. Following treatments with inhibitor or triggers, cells were stained for the detection of ASC specks by Time-of-Flight Inflammasome Evaluation (TOFIE)^{65 (link),66 (link)} using the PE conjugated mouse monoclonal anti-ASC antibody (clone HASC-71, catalog 653903, Biolegend, 1:500). Monocytes were gated using the FITC conjugated mouse monoclonal anti-CD14 antibody (clone M5E2, catalog 557153, BD Biosciences, 1:10) and using the PE-Cy7 conjugated mouse monoclonal anti-CD16 antibody (clone 3G8, catalog 557744, BD Biosciences 1:10). Intracellular ASC-RFP-speck formation in HEK293T cells was evaluated after 24 h post-transfection by TOFIE in different gates with increasing mean fluorescence intensity for NLRP3-YFP (Supplementary Fig. 2D). Human blood samples were analysed by flow cytometry using FACS Canto (BD Biosciences) and for HEK293T cells using LSRFortessa (BD Biosciences), for both cases the FCS express software (De Novo Software) was used.

Molina-López C., Hurtado-Navarro L., García C.J., Angosto-Bazarra D., Vallejo F., Tapia-Abellán A., Marques-Soares J.R., Vargas C., Bujan-Rivas S., Tomás-Barberán F.A., Arostegui J.I, & Pelegrin P. (2024). Pathogenic NLRP3 mutants form constitutively active inflammasomes resulting in immune-metabolic limitation of IL-1β production. Nature Communications, 15, 1096.

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In vitro and histomorphometric analysis of Tabashir on parasites

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The effect of Tabashir on the in vitro growth of parasite was estimated before the in vivo study. Tabashir extract was dissolved in dimethyl sulfoxide (DMSO) and phosphate-buffered saline (PBS), and different concentrations of the extract were prepared. Promastigotes of the parasite were incubated with different concentrations of the extract for 2 hours at the temperature of 4°C and then were collected in Eppendorf tubes and incubated for 30 minutes with propidium iodide (50 g/ml, Sigma™, USA) and then were maintained on ice till analysis. Ethanol solution (70% v/v) was used as control. FACSCalibur flow cytometer (Becton-Dickinson™, USA) and polystyrene flow cytometry tubes (BD Falcon™, USA) were used for flow cytometry.
For the histomorphometric study, nine 1 mm² pieces were cut from the skin samples, were embedded in paraffin blocks, and were cut in 15 μm thickness slices and stained with Heidenhain's azan-trichrome and haematoxylin and eosin (H&E) stains. The volume densities of the collagens, vessels, and follicles of hair were determined by using the stereological point counting method [12 ]. Vascular length density (Lv; mm/mm³), mean vessels' diameter (µm), and fibroblasts' population (Nv; ×10³ per mm³) were estimated as previously reported [13 (link)].

Ghanbarinasab Z., Hosseini-Bensenjan M., Ziabari E.Z., Aminnia S., Borazjani R., Rastegarian Jahromi M., Asgari Q., Sarkari B, & Ashkani-Esfahani S. (2021). Topical Bambusa vulgaris Extract Enhances Wound Healing in Cutaneous Leishmaniasis. Journal of Pathogens, 2021, 7860474.

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Evaluating Toxicity of 2-Indole Derivatives

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2-(naphthalen-e2-ylthio)-1H-indole (Fig. 1) was dissolved in DMSO to obtain a final concentration of 10 mM. The final concentration of DMSO did not exceed 0.2 % v/v. Various concentrations (25–800 μM) of 2-(naphthalene-2-ylthio)-1H-indole were then prepared as follows:
2.5–80 μl of the final concentration was added to 920–997.5μl of suspension that contained 2×10⁶ tachyzoites per ml of PBS. The tachyzoites were incubated with either DMSO (0.2% v/v) as control or the diluted compounds for 1.5 h at 4°C. The tachyzoites were collected in Eppendorf tubes and incubated for 30 min at 4°C with 50 μg/ml propidium iodide (PI) (Sigma Company, USA). After incubation, the parasites were kept on ice until analysis. Positive controls for PI staining were acquired by incubating parasites in the presence of 0.2% saponin (19 ).
The cell suspension was transferred into polystyrene flowcytometry tubes (BD Falcon, USA). Data analysis was performed using FACS Calibur flow cytometer (Becton-Dickinson, San Jose, USA) and Cell Quest Pro software. A total of 10000 or 30000 events were acquired in the region that had been previously established as corresponding to the parasites.

ASGARI Q., KESHAVARZ H., REZAEIAN M., SADEGHPOUR H., MIRI R, & MOTAZEDIAN M.H. (2015). Anti-Toxoplasma Activity of 2-(Naphthalene-2-γlthiol)-1H Indole. Iranian Journal of Parasitology, 10(2), 171-180.

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