P nf κb p65 rabbit monoclonal

The KD-1 (lot: 20,190,201) used in this study was produced and provided by Dalian Fusheng Natural Medicine Development Co. Ltd. (Dalian, China). KD-1 was triturated, and 100 mg was prepared in 1 ml of dimethyl sulfoxide (DMSO). The mixture was ultrasonicated and then centrifuged. The supernatant was passed through a 0.22 μm syringe filter before use. IκBα rabbit monoclonal (lot: 4812), p-IκBα rabbit monoclonal (lot: 2859), NF-κB p65 rabbit monoclonal (lot: 8242), and p-NF-κB p65 rabbit monoclonal (lot: 3033) antibodies were provided by Cell Signaling Technology, Inc. (Danvers, MA, USA).

LS (lot: SA01004C) used in this study was produced and provided by Leiyunshang Pharmaceutical Group Co., Ltd. (Suzhou, China). The LS was triturated, 50 mg was prepared in 10 mL dimethyl sulfoxide (DMSO). The mixture was ultrasonicated for 4 h and then centrifuged at 4000 g for 15 min. The supernatant was filtered through a 0.22 μm syringe filter before use. In the previous study, the index components in LS were detected by high-performance liquid chromatography (HPLC). HPLC revealed that LS contained 0.12 % gamabufotalin, 0.10 % arenobufagin, 0.26 % telocinobufagin, 0.21 % desacetylcinobufotalin, 0.25 % bufotalin, 0.41 % cinobufotalin, 0.27 % bufalin, 0.70 % resibufogenin, 0.68 % cinobufagin, 1.81 % cholic acid, 0.27 % anserine deoxycholic acid, and 0.23 % deoxycholic acid [18 ]. Remdesivir was kindly provided by Prof. Jiancun Zhang from Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences and was dissolved in DMSO to 100 μg/mL and stored at −20 °C before using. IκBα rabbit monoclonal (lot: 4812), p-IκBα rabbit monoclonal (lot: 2859), NF-κB p65 rabbit monoclonal (lot: 8242), p-NF-κB p65 rabbit monoclonal (lot:3033), p38 MAPK rabbit monoclonal (lot: 8690) and p-p38 MAPK rabbit monoclonal (lot: 4631) antibodies were provided by Cell Signaling Technology, Inc. (Danvers, MA, USA).