Alexa fluor 488 anti il 10

Manufactured by Thermo Fisher Scientific

Sourced in United States

Alexa Fluor 488-anti-IL-10 is a fluorescently labeled antibody that specifically binds to the cytokine interleukin-10 (IL-10). It is commonly used in flow cytometry and other immunoassays to detect and quantify IL-10 expression in cell samples.

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Alexa Fluor 488 (6806 citations) Alexa 488 (1863 citations) IL-10 (509 citations) Anti-IL-10 (24 citations) Alexa Fluors (21 citations) Alexa Fluor 488 anti (6 citations) IL-10-Alexa Fluor 488 (2 citations)

2 protocols using alexa fluor 488 anti il 10

Evaluating Th17, Th1, and Th2 Cells in Murine Schistosomiasis

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DCs collected from mice killed at 0 (uninfected control groups were not infected with cercariae), 4 or 7 weeks post‐infection with 12 S. japonicum cercariae and vaccination with NP30 or SEA were cultured with CD4⁺T cells for 24 hours. Lymphocytes were stimulated with PMA and ionomycin. Cells were surface stained with anti‐CD3‐APC and anti‐CD4‐FITC and then intracellularly stained with PE‐conjugated antibodies against IL‐17A, IFN‐γ, IL‐4 or isotype IgG2a control antibody for FACS analysis of Th17, Th1 or Th2 cells. Tregs were detected using the Mouse Regulatory T Cell Staining Kit according to the protocols. To identify and evaluate nonpathogenic Th17 cells, the CD4⁺T cells were intracellularly stained with PE‐anti‐IL‐17A Ab and Alexa Fluor 488‐anti‐IL‐10 according to the manufacturer's instructions (eBioscience, San Diego, CA, USA).

Xu L., Xue B., Zhou L., Qiu Z., Zhang X., Xu N., Tang Q., Zhu J., Guan X, & Feng Z. (2018). NP30 stimulates Th17 differentiation through DC in Schistosomiasis Japonicum. Parasite Immunology, 40(5), e12528.

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Multiparametric Profiling of T Cell Subsets

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eFluor660-anti–IL-21, Alexa Fluor 488–anti–IL-10, PerCP-Cy5.5-anti–IFN-γ, FITC-anti-CD45RA, PE-anti-ICOS, and anti-Tbet, and biotin–PD-1 were from eBioscience. Alexa Fluor 488–anti-GATA3, Alexa Fluor 647–anti-CXCR5, anti-pSTAT4, anti-pSTAT5, anti-pSTAT6, APC-anti-CD10, APC-Cy7-anti-CD4, BV605-anti-IgG, BV421-CD40L, BV711-anti–IL-2, PE-anti-pSTAT1, anti-RORγt, and Bcl-6, PE-Cy7-anti-CD25, and anti-CD27, PerCP-Cy5.5-anti-CD127, anti-pSTAT3, and anti-Tbet, SA-PerCpCy5.5, and IFN-γ were obtained from BD. Pacific Blue–anti-CD20 and SA-BV605 were purchased from BioLegend. Recombinant human IL-12 was purchased from R&D Systems. TGF-β, IL-1β, IL-6, IL-21, and IL-23 were obtained from PeproTech. PGE2 was purchased from Sigma-Aldrich. Human IL-4 and IL-10 were provided by R. de Waal Malefyt (DNAX Research Institute, Palo Alto, CA).

Ma C.S., Wong N., Rao G., Nguyen A., Avery D.T., Payne K., Torpy J., O’Young P., Deenick E., Bustamante J., Puel A., Okada S., Kobayashi M., Martinez-Barricarte R., Elliott M., Sebnem Kilic S., El Baghdadi J., Minegishi Y., Bousfiha A., Robertson N., Hambleton S., Arkwright P.D., French M., Blincoe A.K., Hsu P., Campbell D.E., Stormon M.O., Wong M., Adelstein S., Fulcher D.A., Cook M.C., Stepensky P., Boztug K., Beier R., Ikincioğullari A., Ziegler J.B., Gray P., Picard C., Boisson-Dupuis S., Phan T.G., Grimbacher B., Warnatz K., Holland S.M., Uzel G., Casanova J.L, & Tangye S.G. (2016). Unique and shared signaling pathways cooperate to regulate the differentiation of human CD4+ T cells into distinct effector subsets. The Journal of Experimental Medicine, 213(8), 1589-1608.

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