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Aav9 cag flex tdtomato

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AAV9-CAG-FLEX-tdTomato is a recombinant adeno-associated virus (AAV) vector. The vector contains the CAG promoter and a FLEX switch controlling the expression of the tdTomato fluorescent protein. This product is suitable for Cre-dependent labeling of cells.

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Lab products found in correlation

Aav5 hsyn flex chrimsonr tdtomato, by Addgene (2 mentions) Aav9 ef1a flex tva mcherry, by Addgene (2 mentions) Aav9 cag flex rg, by Addgene (2 mentions) Enva sad δg egfp, by Addgene (2 mentions) Aav2 retro cag icre, by Addgene (2 mentions) Aav9 syn flex jgcamp7s wpre, by Addgene (2 mentions)

3 protocols using aav9 cag flex tdtomato

1

Viral Vector Production and Characterization

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AAV9-hSyn-Flex-GCaMP6s-WPRE-SV40 was produced by the Vector Core of the University of Pennsylvania. AAV9-EF1α-DIO-eNpHR3.0-mCherry, AAV2-CaMKII2α-eNpHR3.0-mCherry, AAV2-CaMKII2α-mCherry, AAV2-EF1α-DIO-hChR2(H134R)-YFP-WPRE and AAV2-EF1α-DIO-mCherry were produced by the Vector Core of the University of North Carolina. AAV5-hSyn-FLEX-ChrimsonR-tdTomato (Addgene plasmid 62723), AAV9-CAG-FLEX-tdTomato (Addgene plasmid # 28306) and AAV9-Syn-Flex-jGCaMP7s-WPRE (Addgene plasmid # 104491) were purchased from Addgene. AAV2(retro)-CAG-iCre (Addgene plasmid # 81070) was produced by Vector Biolabs. AAV9-EF1a-FLEX-TVA-mCherry (Addgene plasmid # 38044) and AAV9-CAG-FLEX-RG (Addgene plasmid # 38043) were produced by Vigene Biosciences, Inc. EnvA-SAD-ΔG-eGFP (Addgene plasmid # 32635) was produced by the Viral Vector Core of the Salk Institute for Biological Studies. All viral vectors were stored in aliquots at −80°C until use.
Ma J., du Hoffmann J., Kindel M., Sofia Beas B., Chudasama Y, & Penzo M.A. (2021). Divergent projections of the paraventricular nucleus of the thalamus mediate the selection of passive and active defensive behaviors. Nature neuroscience, 24(10), 1429-1440.
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2

Viral Vector Production and Characterization

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AAV9-hSyn-Flex-GCaMP6s-WPRE-SV40 was produced by the Vector Core of the University of Pennsylvania. AAV9-EF1α-DIO-eNpHR3.0-mCherry, AAV2-CaMKII2α-eNpHR3.0-mCherry, AAV2-CaMKII2α-mCherry, AAV2-EF1α-DIO-hChR2(H134R)-YFP-WPRE and AAV2-EF1α-DIO-mCherry were produced by the Vector Core of the University of North Carolina. AAV5-hSyn-FLEX-ChrimsonR-tdTomato (Addgene plasmid 62723), AAV9-CAG-FLEX-tdTomato (Addgene plasmid # 28306) and AAV9-Syn-Flex-jGCaMP7s-WPRE (Addgene plasmid # 104491) were purchased from Addgene. AAV2(retro)-CAG-iCre (Addgene plasmid # 81070) was produced by Vector Biolabs. AAV9-EF1a-FLEX-TVA-mCherry (Addgene plasmid # 38044) and AAV9-CAG-FLEX-RG (Addgene plasmid # 38043) were produced by Vigene Biosciences, Inc. EnvA-SAD-ΔG-eGFP (Addgene plasmid # 32635) was produced by the Viral Vector Core of the Salk Institute for Biological Studies. All viral vectors were stored in aliquots at −80°C until use.
Ma J., du Hoffmann J., Kindel M., Sofia Beas B., Chudasama Y, & Penzo M.A. (2021). Divergent projections of the paraventricular nucleus of the thalamus mediate the selection of passive and active defensive behaviors. Nature neuroscience, 24(10), 1429-1440.
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3

Dual AAV Labeling of Dopaminergic Axons

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Two viral vectors, AAV9–CAG–FLEX–tdTomato (Plasmid #28306, Addgene) and AAV9–phSyn1(S)–FLEX–tdTomato–T2A–SypEGFP–WPRE (Plasmid #51509, Addgene), were generated by Boston Children’s Hospital Viral Core at titers of 1.0 × 1013 gc/mL and 4.3 × 1013 gc/mL, respectively. These vectors were injected into the VTA of TH-Cre mice to label dopaminergic axons (tdTomato) and highlight axonal synaptic boutons (SypEGFP). Because the fluorescent intensity of tdTomato expressed by AAV9–phSyn1(S)–FLEX–tdTomato–T2A–SypEGFP–WPRE was relatively weak for visualizing thin dopaminergic axons, AAV9–CAG–FLEX–tdTomato was co-injected to enhance tdTomato expression and facilitate axonal visualization.
Manion M.T., Glasper E.R, & Wang K.H. (2022). A sex difference in mouse dopaminergic projections from the midbrain to basolateral amygdala. Biology of Sex Differences, 13, 75.
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