Antibodies against mmp 3

Tricetin (purity ≥ 99%) was obtained from Extrasynthese, Genay, France. Recombinant rat IL-1β, MTT, and type II collagenase were purchased from Sigma-Aldrich, Merck KGaA, Darmstadt, Germany. Dulbecco's Modified Eagle's Medium (DMEM), penicillin, streptomycin, fetal calf serum (FBS), 0.25% pancreatic enzyme, TRIzol reagent, and cDNA synthesis kit were all purchased from Thermo Fisher Scientific Inc. PGE2 ELISA kit was purchased from R&D Systems, Minneapolis, MN, USA. Caspase-3 cellular activity assay kit, cell lysis buffer, and antibodies against ERK1/2, p-ERK1/2, p38, p-p38, JNK, and p-JNK were purchased from Cell Signaling Technology, Danvers, MA, USA. Antibodies against MMP-3, MMP-13, Bcl-2, and Bax were purchased from Abcam, Cambridge, USA. Antibody against MMP-1 was purchased from Proteintech Group Inc., Rosemont, USA. Griess reagent was purchased from Beyotime, Shanghai, China. SYBR Premix Ex Taq II was purchased from Takara Biotechnology, Dalian, China.

Western blotting was carried out as described [40 ]. Antibodies against Mmp3 and Mmp13 were purchased from Abcam (Cambridge, MA, USA). Antibodies against Mmp10, Dicer1, and Gapdh were purchased from Millipore (Billerica, MA, USA). Antibody against Erk1 was from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Resveratrol (R5010, >99% purity) was purchased from Sigma-Aldrich (St. Louis, MO, USA). CLI-095 (synonymous with TAK-242), R788, and MCC950 were obtained from MedChemExpress (Monmouth Junction, NJ, USA). Antibodies against MMP3 (catalog no. ab52915), MMP9 (catalog no. ab38898), caspase-1 (catalog no. ab1872), IL-1β (catalog no. ab226918), and TLR4 (catalog no. ab8378) were purchased from Abcam (Boston, MA, USA). The antibody against NLRP3 (catalog no. 13158) was obtained from Cell Signaling Technology (Beverly, MA, USA). Antibodies against β-actin (catalog no. sc-47778), TLR4 (catalog no. sc-293072), p21 (catalog no. sc-56335), phosphorylated p53 (p-p53; catalog no. sc-18079), p53 (catalog no. sc-263), and Syk (catalog no. sc-73086) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The antibody against tyrosine phosphorylated 4G10 (catalog no. 05-321) was obtained from Millipore (Billerica, MA, USA). Human MMP3 and MMP9 and mouse MMP9 enzyme-linked immunosorbent assay (ELISA) kits were obtained from R&D Systems (Minneapolis, MN, USA). The mouse MMP3 and mouse IL-1β ELISA kits were obtained from Abcam (Boston, MA, USA).