LC-MS/MS was carried out on nanoAdvance HPLCs (Bruker Daltonik GmbH, Bremen, Germany) attached to either an amaZon speed ETD or a maXis impact mass spectrometer (Bruker). A 5 μL sample was loaded on a C18 trap column (5 μm particles, 200 Å pore size; Bruker) at a flow rate of 5 μL/min. The trap column was then switched in-line with the analytical column (C18, 15 cm, 100 μm ID, 3 μm particles, 200 Å pore size; Bruker), which was held in a column oven at 50 °C, and eluted at a flow rate of 800 nL/min with a gradient from 2 to 45% B in either 43 min (for amaZon speed ETD) or 90 min (for maXis impact). Solvent A was 0.1% formic acid, and solvent B was acetonitrile with 0.1% formic acid. On the amaZon speed ETD, four separate runs were performed on pooled extracts of each sample. In three of the runs, collision-induced dissociation (CID) data were acquired for three MS/MS precursor ions per MS survey scan in one of the mass ranges m/z 350–500, 350–650 or 650–1200. In the fourth run, electron-transfer dissociation (ETD) data were acquired. On the maXis impact, each ultrafiltrate, retentate or PBS tryptic digest was separately analysed, with CID data acquired for five MS/MS precursor ions per MS survey scan in the mass range m/z 350–1200 at a sampling rate of 2–5 Hz.
Maxis impact mass spectrometer
Manufactured by Bruker
Sourced in Germany, United States
The MaXis impact mass spectrometer is a high-resolution time-of-flight mass spectrometer designed for accurate mass measurements and high-throughput analysis. It features a high-performance ion optics system and a fast data acquisition system to provide precise mass determination and enhanced sensitivity.
Automatically generated - may contain errors
Lab products found in correlation
Dpx 500, by Bruker (6 mentions)
Ods am, by YMC (6 mentions)
Rid 20a refractometer, by Shimadzu (5 mentions)
Drx 700, by Bruker (5 mentions)
Lc 20 chromatograph, by Shimadzu (4 mentions)
Perkin elmer 343 polarimeter, by PerkinElmer (4 mentions)
Uv 1601pc spectrometer, by Shimadzu (3 mentions)
Specord uv vis spectrometer, by Zeiss (2 mentions)
Amazon speed etd, by Bruker (1 mentions)
Acclaim pepmap c18 column, by Thermo Fisher Scientific (1 mentions)
Drx 700 spectrometer, by Bruker (1 mentions)
Smartlab x ray diffractometer, by Rigaku (1 mentions)
Advance spectrometer, by Bruker (1 mentions)
Ods a, by YMC (1 mentions)
Si gel60 rp 18 f254s, by Merck Group (1 mentions)
Agilent 1290 hplc system, by Agilent Technologies (1 mentions)
Ultimate 3000 uplc system, by Thermo Fisher Scientific (1 mentions)
Luna hilic 200a, by Phenomenex (1 mentions)
Cary 100 uv vis spectrophotometer, by Agilent Technologies (1 mentions)
Spectrum 65 ft ir spectrometer, by PerkinElmer (1 mentions)
23 protocols using maxis impact mass spectrometer
1
Nanoflow LC-MS/MS Protocol for Protein Analysis
2
Analytical Characterization of Melatonin
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NMR spectra were recorded in DMSO-d6 and acetone-d6 on a Bruker DPX-500 and DRX-700 (Bruker BioSpin GmbH, Rheinstetten, Germany) spectrometers, using TMS as an internal standard. HRESIMS spectra were measured on a Maxis impact mass spectrometer (Bruker Daltonics GmbH, Rheinstetten, Germany).
Low-pressure liquid column chromatography was performed using silica gel (50/100 μm, Imid Ltd., Krasnodar, Russia). Plates (4.5 cm × 6.0 cm) precoated with silica gel (5–17 μm, Imid Ltd., Krasnodar, Russia) were used for thin-layer chromatography. Preparative HPLC was carried out on a Shimadzu LC-20 chromatograph (Shimadzu USA Manufacturing, Canby, OR, USA) with a Shimadzu RID-20A refractometer (Shimadzu Corporation, Kyoto, Japan) using a YMC ODS-AM (YMC Co., Ishikawa, Japan) (5 µm, 10 mm × 250 mm) and YMC SIL (YMC Co., Ishikawa, Japan) (5 µm, 10 mm × 250 mm) columns.
Melatonin (1a ) was purchased from «JSC «PE «Obolenskoe» (Obolensk, Russia).
Low-pressure liquid column chromatography was performed using silica gel (50/100 μm, Imid Ltd., Krasnodar, Russia). Plates (4.5 cm × 6.0 cm) precoated with silica gel (5–17 μm, Imid Ltd., Krasnodar, Russia) were used for thin-layer chromatography. Preparative HPLC was carried out on a Shimadzu LC-20 chromatograph (Shimadzu USA Manufacturing, Canby, OR, USA) with a Shimadzu RID-20A refractometer (Shimadzu Corporation, Kyoto, Japan) using a YMC ODS-AM (YMC Co., Ishikawa, Japan) (5 µm, 10 mm × 250 mm) and YMC SIL (YMC Co., Ishikawa, Japan) (5 µm, 10 mm × 250 mm) columns.
Melatonin (
3
Characterization of Natural Compounds
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Optical rotations were measured on a Perkin-Elmer 343 polarimeter (Perkin Elmer, Waltham, MA, USA). UV spectra were recorded on a Specord UV−vis spectrometer (Carl Zeiss, Jena, Germany) in methanol. NMR spectra were recorded in CDCl3, acetone-d6 and DMSO-d6 with Bruker DPX-500 (Bruker BioSpin GmbH, Rheinstetten, Germany) and Bruker DRX-700 (Bruker BioSpin GmbH, Rheinstetten, Germany) spectrometers, using TMS as an internal standard. HRESIMS spectra were measured on a Maxis impact mass spectrometer (Bruker Daltonics GmbH, Rheinstetten, Germany).
Low-pressure liquid column chromatography was performed using silica gel (50/100 μm, Imid, Russia). Plates (4.5 cm × 6.0 cm) precoated with silica gel (5–17 μm, Imid) were used for thin-layer chromatography. Preparative HPLC was carried out with a Shimadzu LC-20 chromatograph (Shimadzu USA Manufacturing, Canby, OR, USA) using YMC ODS-AM (YMC Co., Ishikawa, Japan) (5 µm, 10 mm × 250 mm) and YMC SIL (YMC Co., Ishikawa, Japan) (5 µm, 10 mm × 250 mm) columns with a Shimadzu RID-20A refractometer (Shimadzu Corporation, Kyoto, Japan).
Low-pressure liquid column chromatography was performed using silica gel (50/100 μm, Imid, Russia). Plates (4.5 cm × 6.0 cm) precoated with silica gel (5–17 μm, Imid) were used for thin-layer chromatography. Preparative HPLC was carried out with a Shimadzu LC-20 chromatograph (Shimadzu USA Manufacturing, Canby, OR, USA) using YMC ODS-AM (YMC Co., Ishikawa, Japan) (5 µm, 10 mm × 250 mm) and YMC SIL (YMC Co., Ishikawa, Japan) (5 µm, 10 mm × 250 mm) columns with a Shimadzu RID-20A refractometer (Shimadzu Corporation, Kyoto, Japan).
4
High-Resolution Electrospray Ionization Mass Spectrometry
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LC-HR-ESI-MS were acquired using a maXis impact mass spectrometer (Bruker Daltonics, Billerica, MA, USA) coupled to a 1290 Agilent LC (Agilent) using a quadrupole time-of-flight mass detector equipped with an electrospray ionization interface controlled by Bruker software. Column fractions and purified compounds (2 µL) in MeOH were separated on Agilent Eclipse Plus RP-C18 (2.1 × 50 mm; 1.8µm) (Agilent) at 40 °C with a flow rate of 0.2 mL/min. The column was eluted with 0.1% formic acid in water (A) and 0.1% formic acid in acetonitrile (B) as follows, 2–95% B for 0–9 min, 95–2%B for 9–11 min, the column was re-equilibrated for 2 min before the next injection. All acquisitions were performed under positive ionization mode with a capillary voltage of 4200 V. Nitrogen was used as nebulizer gas (4.0 bar) and, as well as drying gas at 12 L/min, source temperature was maintained at 250 °C. Full scan mass spectra were acquired from m/z 50–2000. Data processing was done using Data Analysis Version 4.3.
5
Proteomic Analysis of Treated Cells
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The crude extracts of treated and non-treated cells were stained with Cy5 and Cy3 dyes, respectively. Next, the first dimension of two-dimensional gel electrophoresis was performed by using ready IPG gel strips with pH gradient of 4-7 (Bio-Rad); the second dimension was carried out in 15%-polyacrylamide gel by [42 (link)]. The protein spots were excised from the gel, subjected to in-gel trypsinolysis, and identified using LC-MS/MS on a maXis Impact™ Mass Spectrometer (Bruker). Polyacrylamide gel electrophoresis under native conditions was performed as described in [42 (link)] with IbpA from Acholeplasma laidlawii [43 (link)], glutamine synthetase (GS) from Bacillus subtilis [44 (link)], DNAase, bovine serum albumin (BSA) from Bos taurus, and bovine liver catalase as model proteins. The Gs activity was measured as described in [44 (link)].
6
Peptide Identification by LC-MS/MS
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Liquid chromatography–tandem mass spectrometry (LC-MS/MS) was performed using a chromatography system Dionex Ultimate 3000 (Thermo Scientific Dionex, Waltham, MA, USA) coupled to a maXis Impact mass spectrometer (Bruker Daltonik GmbH, Bremen, Germany) with a Captive Spray electrospray ionization platform. Trypsin-digested peptides were separated using an AcclaimPepMap C18 column (2 µm, 100 Å, 75 µm × 15 cm; Thermo Scientific, Waltham, MA, USA). The mobile phases consisted of 0.1% formic acid and 5% acetonitrile in water (solvent A) and 0.1% formic acid in 94.9% acetonitrile (solvent B). Chromatographic separation was performed in several steps: 0–5 min, 2% solvent B; 5–160 min, gradient from 2 to 55% solvent B; 160–165 min, gradient from 55 to 90% solvent B; 165–180 min, 90% solvent B; 180–190 min, gradient from 90 to 2% solvent B; and 190–195 min, 2% solvent B. The flow rate was 300 nL/min. The separation temperature was 40 °C. A spectrum of positively charged ions was obtained using the following settings: 3 L/min gas flow rate, 150 °C temperature, 1600 V voltage, a detection range of 50–2200 m/z, 10 Hz sampling frequency, and automatic ion fragmentation in MS/MS mode.
7
Characterization of Organic Compounds
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Optical rotations were measured on a Perkin-Elmer 343 polarimeter (Perkin Elmer, Waltham, MA, USA). UV spectra were recorded on a Shimadzu UV-1601PC spectrometer (Shimadzu Corporation, Kyoto, Japan) in methanol. CD spectra were measured with a Chirascan-Plus CD spectrometer (Leatherhead, UK) in methanol. NMR spectra were recorded on a Bruker DRX-700 spectrometer (Bruker BioSpin GmbH, Rheinstetten, Germany), using TMS as an internal standard. HRESIMS spectra were measured on a Maxis Impact mass spectrometer (Bruker Daltonics GmbH, Rheinstetten, Germany).
Low-pressure liquid column chromatography was performed using silica gel (50/100 μm, Imid Ltd., Krasnodar, Russia). Plates (5 × 10.0 cm) precoated with silica gel (5–17 μm, Imid Ltd., Krasnodar, Russia) were used for thin-layer chromatography. Preparative HPLC was carried out on a Shimadzu LC-20 chromatograph (Shimadzu USA Manufacturing, Canby, OR, USA) using a YMC ODS-AM (YMC Co., Ishikawa, Japan) (5 µm, 10 mm × 250 mm) column with a Shimadzu RID-20A refractometer (Shimadzu Corporation, Kyoto, Japan).
Low-pressure liquid column chromatography was performed using silica gel (50/100 μm, Imid Ltd., Krasnodar, Russia). Plates (5 × 10.0 cm) precoated with silica gel (5–17 μm, Imid Ltd., Krasnodar, Russia) were used for thin-layer chromatography. Preparative HPLC was carried out on a Shimadzu LC-20 chromatograph (Shimadzu USA Manufacturing, Canby, OR, USA) using a YMC ODS-AM (YMC Co., Ishikawa, Japan) (5 µm, 10 mm × 250 mm) column with a Shimadzu RID-20A refractometer (Shimadzu Corporation, Kyoto, Japan).
8
Comprehensive Materials Characterization of Copper Nanoparticles
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Transmission electron microscopy (TEM) was performed by Tecnai G2 T20 S-Twin TEM under 200 keV. High-angle annular dark-field (HAADF) scanning TEM-energy-dispersive X-ray spectroscopy (HAADF-STEM-EDX) maps was collected by an FEI Titan Cubed Themis G2 60–300 microscope at an accelerating voltage of 200 keV. X-ray powder diffraction (XRD) spectrum was obtained by using Rigaku SmartLab X-ray diffractometer using Cu Kα radiation. Photoluminescence (PL) spectrum was measured by a Flourolog-3 Fluorometer at room temperature, under 455 nm excitation wavelength and UV-vis absorption spectroscopy was executed with an Agilent Cary 5000 UV-vis-NIR spectrophotometer. Nuclear Magnetic Resonance (NMR): 31P{1H} spectra were recorded on a 300 MHz Bruker Advance spectrometer with CDCl3 as the deuterated solvent. Different amount of Cu (0.02 mmol, 0.04 mmol, 0.4 mmol and 1 mmol) was dissolved into 2 mL of TOP at 150 °C to prepare TOP2–Cu stock solution. The TOP or TOP2–Cu stock solution (0.5 mL) was diluted with 0.5 mL of CDCl3. An external standard H3PO4 (85%) was used for calibrating the 31P NMR spectrum. Electrospray ionization-mass spectrometry (ESI-MS) measurements were performed on a maXis Impact mass spectrometer (Bruker). TOP or TOP2–Cu (0.04 mmol) stock solution (0.8 mL) was diluted with 1 mL of chloroform.
9
Characterization of Natural Products
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Optical rotations were measured on a Perkin-Elmer 343 polarimeter (Perkin Elmer, Waltham, MA, USA). UV spectra were recorded on a Shimadzu UV-1601PC spectrometer (Shimadzu Corporation, Kyoto, Japan) in methanol. CD spectra were measured with a Chirascan-Plus CD Spectrometer (Leatherhead, UK) in methanol. NMR spectra were recorded in CD3OD and CDCl3, on a Bruker DPX-500 (Bruker BioSpin GmbH, Rheinstetten, Germany) and a Bruker DRX-700 (Bruker BioSpin GmbH, Rheinstetten, Germany) spectrometer, using TMS as an internal standard. HRESIMS spectra were measured on a Maxis impact mass spectrometer (Bruker Daltonics GmbH, Rheinstetten, Germany).
Low-pressure liquid column chromatography was performed using Si gel L (50/100 μm, Imid, Russia) and Gel ODS-A (12 nm, S – 75 um, YMC Co, Ishikawa, Japan). Plates precoated with Si gel (5–17 μm, 4.5 × 6.0 cm, Imid) and Si gel60 RP-18 F254S (20 × 20 cm, Merck KGaA, Germany) were used for thin-layer chromatography. Preparative HPLC was carried out on aAgilent 1100 chromatograph (Agilent Technologies, USA) using a YMC ODS-AM (YMC Co., Ishikawa, Japan) (5 µm, 10 × 250 mm), YMC ODS-A (YMC Co., Ishikawa, Japan) (5 µm, 4.6 × 250 mm) and Supelco Discovery C-18 (5 μm, 250 × 4.6 mm) columns with a Agilent 1100 refractometer (Agilent Technologies, Santa Clara, CA, USA).
Low-pressure liquid column chromatography was performed using Si gel L (50/100 μm, Imid, Russia) and Gel ODS-A (12 nm, S – 75 um, YMC Co, Ishikawa, Japan). Plates precoated with Si gel (5–17 μm, 4.5 × 6.0 cm, Imid) and Si gel60 RP-18 F254S (20 × 20 cm, Merck KGaA, Germany) were used for thin-layer chromatography. Preparative HPLC was carried out on aAgilent 1100 chromatograph (Agilent Technologies, USA) using a YMC ODS-AM (YMC Co., Ishikawa, Japan) (5 µm, 10 × 250 mm), YMC ODS-A (YMC Co., Ishikawa, Japan) (5 µm, 4.6 × 250 mm) and Supelco Discovery C-18 (5 μm, 250 × 4.6 mm) columns with a Agilent 1100 refractometer (Agilent Technologies, Santa Clara, CA, USA).
10
Anthocyanin Analysis by HPLC-MS
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Anthocyanins were analyzed by 1290 Agilent LC system (Agilent, Santa Clara, CA, USA). A C18 column, rapid resolution high definition (RRHD) Zorbax Eclipse plus (1.8 μm; 50 × 2.1 mm) (Agilent, Santa Clara, CA, USA), was used for the chromatographic separation. The gradient mobile phase consisted of A) 0.1% formic acid in water and (B) 0.1% formic acid in acetonitrile. Mass spectral analysis was performed according to the published papers [69 (link)]. Briefly, a maXis impact mass spectrometer (Bruker Daltonics, Billerica, MA) was used for obtaining the mass spectra in ESI in positive ionization mode. MS and bbCID data were obtained at m/z 50–2000 scan range. Nitrogen was used as a nebulizer (gas pressure of 2.1 bar) and drying gas (8.0 L/min). The capillary ion voltage was 4500 V, and the drying gas temperature was 250 °C. External mass spectrometer calibration was performed by sodium formate solution. The determination of accurate mass data for the molecular ions was performed using the software Data Analysis 4.3.
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