Mice were housed in AAALAC-accredited vivarium. Room temperature was maintained at 22 ± 3 °C and the humidity at 55 ± 15%. Animals were kept at a 12 h/12 h dark/light cycle. The C57BL/6 and SCID/Beige mice were purchased from Charles River (Wilmington, MA). Nude mice, Col1a2-CreERT2, C57BL/6-Tg(TRAMP)8247Ng/J, and B6.Cg-Gt(ROSA)26Sortm3(CAG-EYFP)Hze/J, Ptentm1Hwu, and R26-LSL-KrasG12D mice were purchased from the Jackson Laboratory (Bar Harbor, ME). The ARR2Pb-Cre mice were from Dr. Fen Wang at the Institute of Biosciences and Technology at Texas A&M. The R26-LSL-Foxf2 mice were generated at the Baylor College of Medicine. The Foxf2 null mouse embryos were generously provided by Dr. Rulang Jia at Cincinnati Children’s Hospital. All mice were on the C57BL/6 background. Mice were genotyped by polymerase chain reaction using mouse genomic DNA from tail biopsy specimens. The sequences of genotyping primers and the expected band sizes for PCR are listed in Supplementary Table 2 . PCR products were separated electophoretically on 1% agarose gels and visualized via ethidium bromide under UV light.
C57bl 6 tg tramp 8247ng j
Manufactured by Jackson ImmunoResearch
Sourced in United States, Montenegro
C57BL/6-Tg(TRAMP)8247Ng/J is a transgenic mouse strain that expresses the SV40 T antigen under the control of the probasin promoter. This strain is commonly used in prostate cancer research.
Automatically generated - may contain errors
Lab products found in correlation
Col1a2 creert2, by Jackson ImmunoResearch (2 mentions)
Jax nsg mice 572ncg, by Charles River Laboratories (2 mentions)
Ptentm1hwu, by Jackson ImmunoResearch (1 mentions)
Scid beige, by Charles River Laboratories (1 mentions)
B6 cg gt rosa 26sortm3 cag eyfp hze j, by Jackson ImmunoResearch (1 mentions)
C57bl 6, by Charles River Laboratories (1 mentions)
C57bl 6j mice, by Jackson ImmunoResearch (1 mentions)
Cb17 scid mice, by Inotiv (1 mentions)
Matrigel matrix, by Corning (1 mentions)
B6 sjl ptprca pepcb boyj, by Jackson ImmunoResearch (1 mentions)
C57bl 6j b, by Jackson ImmunoResearch (1 mentions)
Fvb n tg mmtv pyvt 634mul j, by Jackson ImmunoResearch (1 mentions)
B6 cg ndor1 tg, by Jackson ImmunoResearch (1 mentions)
Pd l1 antibody, by BioXCell (1 mentions)
Fvb nj mice, by Jackson ImmunoResearch (1 mentions)
Extract n amp tissue pcr kit, by Merck Group (1 mentions)
C56bl 6j, by Jackson ImmunoResearch (1 mentions)
C57bl 6 mice, by Charles River Laboratories (1 mentions)
B6 cg terctm1rdp j, by Jackson ImmunoResearch (1 mentions)
Tg krt14 cre 1amc j, by Jackson ImmunoResearch (1 mentions)
17 protocols using c57bl 6 tg tramp 8247ng j
1
Genotyping of Transgenic Mouse Lines
2
Preclinical Prostate Cancer Xenograft Study
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All mice were housed in accordance with the protocol approved by the Institutional Animal Care and Use Committee at Wake Forest University Health Sciences (Winston-Salem, NC, USA). TRAMP mice (C57BL/6-Tg(TRAMP)8247Ng/J) were purchased from Jackson Labs (Bar Harbor, ME, USA) at 12 weeks of age and were given ad libitum food and water on a 12 h light–dark cycle. Mice were sacrificed at 44 weeks of age. PC3-PTXR (paclitaxel-resistant) and 22Rv1 xenografts were reported previously by our lab [22 (link),23 (link)]. MDA-PCa-118-B 7c (PDX118) and MDA-PCa-174-6-5a (PDX174) were obtained from the Tissue Biospecimen and Pathology Resource at MD Anderson Cancer Center (Houston, TX, USA) and maintained in CB17-SCID mice (Envigo, Indianapolis, IN, USA).
C57BL/6J mice were purchased from Jackson Labs (Bar Harbor, Maine) at 5–6 weeks of age. For allograft generation, 3 × 106 TRAMP-C1 cells in 50 µL PBS were mixed with 50 µL Matrigel matrix (cat# 354248, Corning, NY, USA) and injected subcutaneously and bilaterally into flanks. After one week, mice received intraperitoneal injections of either MK-8931 (30 mg/kg) or PBS five days a week for five weeks. Tumor growth was assessed with calipers, and volume (mm3) was calculated as width (mm)2 × length (mm) × 0.52. Mice were sacrificed at the end and tissues were collected.
C57BL/6J mice were purchased from Jackson Labs (Bar Harbor, Maine) at 5–6 weeks of age. For allograft generation, 3 × 106 TRAMP-C1 cells in 50 µL PBS were mixed with 50 µL Matrigel matrix (cat# 354248, Corning, NY, USA) and injected subcutaneously and bilaterally into flanks. After one week, mice received intraperitoneal injections of either MK-8931 (30 mg/kg) or PBS five days a week for five weeks. Tumor growth was assessed with calipers, and volume (mm3) was calculated as width (mm)2 × length (mm) × 0.52. Mice were sacrificed at the end and tissues were collected.
3
Genetically Engineered Mouse Models
4
Evaluating DMAMCL and Radiation Therapy in TRAMP Mice
5
Prostate Cancer Treatment in TRAMP Mice
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TRAMP mice (C57BL/6-Tg(TRAMP)8247Ng/J) [42 (link)] were obtained from The Jackson Laboratory (Bar Harbor, ME, USA) and bred as described in [19 (link)]. Twelve-week-old mice were treated i.p. with iron dextran 40 mg/kg/week, RSL3 (dissolved in DMSO) 10 mg/kg/week or RSL3 + iron starting at 12 week of age until 25 week of age.
For combination studies with androgen deprivation therapy, TRAMP mice were treated with enzalutamide (3 mg/kg) in the drinking water from 12 week of age until 25 week of age in combination or not with sub-optimal doses of RSL3 + iron dextran (5 mg/kg/week RSL3, 10 mg/kg/week iron dextran). At week 25 mice were sacrificed and prostates processed as described in [20 (link), 43 (link)].
For combination studies with androgen deprivation therapy, TRAMP mice were treated with enzalutamide (3 mg/kg) in the drinking water from 12 week of age until 25 week of age in combination or not with sub-optimal doses of RSL3 + iron dextran (5 mg/kg/week RSL3, 10 mg/kg/week iron dextran). At week 25 mice were sacrificed and prostates processed as described in [20 (link), 43 (link)].
6
Prostate Cancer Mouse Models
7
Genetically Engineered Murine Cancer Model
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The University of Illinois Laboratory Institutional Animal Care and Use Committee reviewed and approved all animal procedures (protocol #19011), which were carried out in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals and reported according to the ARRIVE guidelines. Male C57Bl/6-Tg(TRAMP)8247Ng/J and female FVB/NJ mice were purchased from The Jackson Laboratory (Bar Harbor, ME, USA) and bred to produce offspring ([C57Bl/6 × FVB]F1). The hemizygous TRAMP genotype was confirmed by tail DNA analysis, which was isolated with Extract-N-Amp Tissue PCR kits (Sigma-Aldrich, St. Louis, MO, USA) and genotyped according to the primer sequences listed in Supplementary Table S2 . Mice were housed under controlled conditions (12-h light/dark cycle, 22ºC, 55% humidity), weighed weekly, and given fresh diet three times per week.
8
TRAMP Mice Prostate Analysis
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C57BL/6 TRAMP mice (C57BL/6-Tg(TRAMP)8247Ng/J) were purchased from the Jackson Laboratory and Ebag9KO mice (Ebag9−/−) were described previously13 (link). TRAMP mice were crossed with Ebag9KO mice to generate Ebag9KO;TRAMP(+) mice. At 1 year old, mice were killed and the genitourinary (GU) complex (consisting of prostate, seminal vesicles, urethra, and empty bladder) was weighed. Mice were kept under specific pathogen-free conditions and fed dry food and water ad libitum. The results are shown as mean values ± SD (Ebag9KO;TRAMP(+); n = 10, Ebag9WT;TRAMP(+); n = 17). Statistical analysis was carried out using the modified Thompson tau technique and two-sided Student’s t-test. *P < 0.05. All experiments were performed according to the guidelines for the care and use of laboratory animals of Saitama Medical University. Animals were randomly assigned to the groups and the experiments were performed without blinding. The sample size estimate was based on our pilot study.
9
TRAMP Mouse Model for Prostate Cancer
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B6 TRAMP
mice (C57BL/6-Tg(TRAMP)8247Ng/J)
breeding pairs from the Jackson Laboratory were housed and bred as
instructed. The TRAMP offspring strain mice were selected by genotyping.
Twenty-four week-old TRAMP mice were i.v. injected with PBS, OxPt
plus IRI plus αPD-L1, OxPt/SN38, or OxPt/SN38 plus αPD-L1
once every 3 days for 10 doses. A 75 μg portion of αPD-L1
was i.p. injected in the same schedule. Thirty days after the first
injection, the prostates and seminal vehicles (genitourinary tracts)
were dissected and normalized against the mouse body weight.
mice (C57BL/6-Tg(TRAMP)8247Ng/J)
breeding pairs from the Jackson Laboratory were housed and bred as
instructed. The TRAMP offspring strain mice were selected by genotyping.
Twenty-four week-old TRAMP mice were i.v. injected with PBS, OxPt
plus IRI plus αPD-L1, OxPt/SN38, or OxPt/SN38 plus αPD-L1
once every 3 days for 10 doses. A 75 μg portion of αPD-L1
was i.p. injected in the same schedule. Thirty days after the first
injection, the prostates and seminal vehicles (genitourinary tracts)
were dissected and normalized against the mouse body weight.
10
Prostate Cancer Mouse Models
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Animal handling and experimental procedures conformed to institutional guidelines and were approved by the Sanford-Burnham-Prebys Medical Discovery Institute Institutional Animal Care and Use Committee, and by the Weill Cornell Medicine Institutional Animal Care and Use Committee. For Olaparib treatment experiments, 13 week-old male TRAMP+ mice (C57BL/6-Tg(TRAMP) 8247 Ng/J, stock No: 003135) were purchased from The Jackson Laboratory, (Bar Harbor, ME, USA). TRAMP+ mice were generated in a C57BL/6 background and were born and maintained under pathogen-free conditions. All genotyping was done by PCR. Age-matched mice were used for all experiments. For xenograft experiments, 7-week-old male JAX NSG mice (572NCG) were purchased from Charles River Labs (Wilmington, MA, USA). NSG mice were purchased and maintained under pathogen-free conditions. All mice were maintained on food and water ad libitum and were age-matched and co-housed for all experiments. Mice were sacrificed and prostate, tumors or other organs were collected for analysis.
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