Log In Sign up
The largest database of trusted experimental protocols

Hami3379

Manufactured by Cayman Chemical
Visit Supplier
Sourced in United States

HAMI3379 is a laboratory equipment product offered by Cayman Chemical. It is a device designed for a specific function within the scientific research and analysis workflow. The core purpose of this product is to facilitate a particular step or procedure commonly performed in laboratory settings. No further details or interpretations about the intended use or application of this product are provided.

Automatically generated - may contain errors

Lab products found in correlation

Mk571, by Cayman Chemical (3 mentions) Montelukast, by Merck Group (2 mentions) Montelukast, by Cayman Chemical (1 mentions) Gw9662, by Cayman Chemical (1 mentions) Calcium ionophore a23187, by Merck Group (1 mentions) Pertussis toxin, by Merck Group (1 mentions) T0070907, by Cayman Chemical (1 mentions) U0126, by Cayman Chemical (1 mentions) Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (1 mentions) Erastin, by Merck Group (1 mentions) Cresyl violet, by Merck Group (1 mentions) Pranlukast, by Cayman Chemical (1 mentions) Zafirlukast, by Merck Group (1 mentions) Clopidogrel, by Merck Group (1 mentions) Mk886, by Cayman Chemical (1 mentions) Everolimus, by Merck Group (1 mentions) Mda mb 231, by American Type Culture Collection (1 mentions) Mda 231, by American Type Culture Collection (1 mentions) D2390, by Merck Group (1 mentions) Bltr1 antibody, by Biorbyt (1 mentions)

9 protocols using hami3379

1

Leukotrienes Signaling Pathway Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Leukotrienes (LTC4, LTD4 and LTE4), Montelukast, MK-571, HAMI3379, U-0126, GW9662, T0070907 (all Cayman Chemical), EDTA (Ambion), BAPTA-AM, Pertussis Toxin, Calcium ionophore (A23187), (all Sigma-Aldrich) were obtained from the manufacturers.
Foster H.R., Fuerst E., Branchett W., Lee T.H., Cousins D.J, & Woszczek G. (2016). Leukotriene E4 is a full functional agonist for human cysteinyl leukotriene type 1 receptor-dependent gene expression. Scientific Reports, 6, 20461.
+ Open protocol
+ Expand
2

Preparation of Drug Stock Solutions

Check if the same lab product or an alternative is used in the 5 most similar protocols
Quininib, 1,4-dihydroxy quininib, montelukast (Sigma-Aldrich, St. Louis, MO, USA #SML0101), HAMI 3379 (Cayman Chemical, Ann Arbor, MI, USA #10580) and erastin (Sigma-Aldrich; St. Louis, MO, USA #E7781) were dissolved in 100% DMSO and stored as (10–50 mM) stock solutions. Working solutions (100 μM) were prepared fresh prior to each experiment in complete cell culture medium as described above. Drugs were made to final test concentrations by adding the required volume of the working solution to cells in complete media. 0.5% DMSO was used as a control for all other drug treatment experiments.
Tonelotto V., Costa-Garcia M., O’Reilly E., Smith K.F., Slater K., Dillon E.T., Pendino M., Higgins C., Sist P., Bosch R., Passamonti S., Piulats J.M., Villanueva A., Tramer F., Vanella L., Carey M, & Kennedy B.N. (2024). 1,4-dihydroxy quininib activates ferroptosis pathways in metastatic uveal melanoma and reveals a novel prognostic biomarker signature. Cell Death Discovery, 10, 70.
+ Open protocol
+ Expand
3

Serotype III Pneumococcus Infection Model

Check if the same lab product or an alternative is used in the 5 most similar protocols
Pranlukast (purity 99.89%) and HAMI 3379 (purity 98%) were purchased from Cayman Chemical Company. Cresyl violet was purchased from Sigma-Aldrich; Merck KGaA. All other reagents used in the experiment were of analytical grade.
The Chinese Institute for the Control of Pharmaceutical and Biological Products (Beijing, China) provided the SP serotype III standard strain. The bacteria were cultured overnight at 37˚C on sheep blood agar plates under anaerobic conditions (H2 and CO2; 95:5, v/v). Then, selected single colonies were cultured overnight to logarithmic phase in broth at 37˚C and harvested by centrifugation at 2,500 x g for 10 min at 4˚C. Bacteria suspension was adjusted to 107 colony forming units (CFU)/ml with sterile saline for intracisternal injection.
Yu S., Chen X., Li X., Yan J, & Jiang Y. (2022). Neuroprotective effects of CysLTR antagonist on Streptococcus pneumoniae-induced meningitis in rats. Experimental and Therapeutic Medicine, 24(1), 443.
+ Open protocol
+ Expand
4

Osteotropic Metastatic Breast Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
Two osteotropic metastatic human breast cancer cell lines were used in this study: MDA-MB-231/B02 (MDA-B02) and a subclone expressing the three markers GFP, luciferase, and a beta-galactosidase referred to as MDA-B02-Luc for simplification and which was described previously [46 (link)], as well as their parental breast cancer cell line MDA-MB-231 (MDA-231) and the murine 4T1 breast cancer cells obtained from the American Type Culture Collection (ATCC®). The 4T1 cells derive from a BALB/c spontaneous mammary carcinoma and are naturally resistant to 6-thioguanine [47 (link)]. They were maintained in Dulbecco’s Modified Eagle Medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. Zafirlukast and montelukast, the CysLT1R antagonists, everolimus, the mTORC1 inhibitor, and clopidogrel, the P2Y12 inhibitor, were purchased from Sigma. HAMI3379, the CysLT2R antagonist, and MK-886, the FLAP inhibitor, were from Cayman. LTD4 was from Bertin.
Saier L., Ribeiro J., Daunizeau T., Houssin A., Ichim G., Barette C., Bouazza L, & Peyruchaud O. (2022). Blockade of Platelet CysLT1R Receptor with Zafirlukast Counteracts Platelet Protumoral Action and Prevents Breast Cancer Metastasis to Bone and Lung. International Journal of Molecular Sciences, 23(20), 12221.
+ Open protocol
+ Expand
5

Pharmacological Compound Preparation and Handling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Quininib (Q1), 1,4-dihydroxy quininib (Q7) (30 (link), 34 (link)), montelukast (Sigma #SML0101), HAMI 3379 (Cayman Chemical #10580), and dacarbazine (Sigma #D2390) were dissolved in 100% DMSO and stored as (10–50 mM) stock solutions. Working solutions (100 μM) were prepared fresh prior to each experiment in complete cell culture medium as described above. Drugs were made to final test concentrations by adding the required volume of the working solution to cells in complete media. 0.5% DMSO was used as vehicle control.
Slater K., Bosch R., Smith K.F., Jahangir C.A., Garcia-Mulero S., Rahman A., O’Connell F., Piulats J.M., O’Neill V., Horgan N., Coupland S.E., O’Sullivan J., Gallagher W.M., Villanueva A, & Kennedy B.N. (2023). 1,4-dihydroxy quininib modulates the secretome of uveal melanoma tumour explants and a marker of oxidative phosphorylation in a metastatic xenograft model. Frontiers in Medicine, 9, 1036322.
+ Open protocol
+ Expand
6

IL-33 and LTC4 Modulate Mast Cell Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Laboratory of Allergic Disease-2 (LAD2) mast cells [19 (link)] were treated with human IL-33 and/or LTC4 for 4 h and in some conditions, a cysteinyl leukotriene (CysLT)1 receptor antagonist (LT1RA) MK571 (5 μM) and/or a CysLT2 receptor antagonist (LT2RA) HAMI3379 (5 μM) (Cayman Chemical, Ann Arbor, MI, USA) were added before treatment. TaqMan-based PCR analysis quantified the expression of IL13, CMA1, TPSAB1 and CPA3 genes relative to the endogenous control gene.
Al-Shaikhly T., Murphy R.C., Parker A., Lai Y., Altman M.C., Larmore M., Altemeier W.A., Frevert C.W., Debley J.S., Piliponsky A.M., Ziegler S.F., Peters M.C, & Hallstrand T.S. (2022). Location of eosinophils in the airway wall is critical for specific features of airway hyperresponsiveness and T2 inflammation in asthma. The European respiratory journal, 60(2), 2101865.
+ Open protocol
+ Expand
7

HMGB1 and Leukotriene Pathway Modulators

Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant human HMGB1 antibody was purchased from R&D System Inc. (Minneapolis, MN, USA). Zileuton (( ±)-N-hydroxy-N-(1-benzo[b]thien-2-ylethyl)urea) was purchased from Sigma-Aldrich Inc. (St. Louis, MO, USA). The MCP-1 antibody was purchased from Invitrogen (Carlsbad, CA, USA) and BLTR1 antibody was purchased from Biorbyt (Cambridge, UK). 5-LO and β-actin antibodies were purchased from Santa Cruz Biotechnology Inc. (Beverly, MA, USA). LTB4, LTC4, BLTR1 inhibitor (U75302), BLTR2 inhibitor (LY255283), LTC4 receptor (CysLTR) 1 inhibitor (Montelukast) and CysLTR2 inhibitor (HAMI3379) were purchased from Cayman Chemical Inc. (Ann Arbor, MI, USA). Horseradish peroxidase (HRP)-conjugated IgG antibody (Santa Cruz Biotechnology Inc.) was used as secondary antibody.
Choi J.M., Baek S.E., Kim J.O., Jeon E.Y., Jang E.J, & Kim C.D. (2021). 5-LO-derived LTB4 plays a key role in MCP-1 expression in HMGB1-exposed VSMCs via a BLTR1 signaling axis. Scientific Reports, 11, 11100.
+ Open protocol
+ Expand
8

Evaluating Small Molecule Anticancer Drugs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Quininib (Q1), 1,4-dihydroxy quininib (Q7) [32 (link),33 (link)], montelukast (Mon) (Sigma-Aldrich, St. Louis, MO, USA #SML0101), HAMI 3379 (Cayman Chemical, Ann Arbor, MI, USA #10580) and dacarbazine (Sigma-Aldrich, St. Louis, MO, USA #D2390) were dissolved in 100% DMSO and stored as (10–50 mM) stock solutions. Working solutions (100 μM) were prepared fresh prior to each experiment in complete cell culture medium as described above. Drugs were made to final test concentrations by adding the required volume of the working solution to cells in complete media.0.1% and 0.2% DMSO were used as controls in Seahorse Assay experiments.0.5% DMSO was used as a control for all other drug treatment experiments.
Slater K., Heeran A.B., Garcia-Mulero S., Kalirai H., Sanz-Pamplona R., Rahman A., Al-Attar N., Helmi M., O’Connell F., Bosch R., Portela A., Villanueva A., Gallagher W.M., Jensen L.D., Piulats J.M., Coupland S.E., O’Sullivan J, & Kennedy B.N. (2020). High Cysteinyl Leukotriene Receptor 1 Expression Correlates with Poor Survival of Uveal Melanoma Patients and Cognate Antagonist Drugs Modulate the Growth, Cancer Secretome, and Metabolism of Uveal Melanoma Cells. Cancers, 12(10), 2950.
+ Open protocol
+ Expand
9

Murine Allergic Inflammation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Extract from Dermatophagoides farinae (Df) was obtained from Greer Laboratories (XPB81D3A25; Lenoir, NC). Ovalbumin (OVA) and PBS were obtained from Sigma-Aldrich (St Louis, Mo). The mMCP-1 EIA kit was purchased from eBiosciences (San Diego, Calif). LTA4, LTC4, LTD4, LTE4, MK571, and HAMI3379 were obtained from Cayman Chemical (Ann Arbor, Mich). Histamine, thromboxane receptor B2, PGD2, and cysLT EIA kits were obtained from Cayman. IL-4, IL-5, IL-13, ICAM-1, and VCAM-1 EIA kits were from R&D Systems (Minneapolis, Minn). The CXCL7 EIA kit was purchased from Abcam (Cambridge, Mass). The HMGB1 EIA kit was from LifeSpan (Providence, RI). The monoclonal goat anti-mouse IL-33 was purchased from R&D Systems (Minneapolis, Minn), and the rat anti-mouse IgG (H1L) secondary antibody, fluorescein isothiocyanate (FITC) anti-mouse CD11c, FITC anti-mouse/human CD11b, FITC anti-mouse IgE, FITC anti-mouse CD3ε, FITC anti-mouse CD19, FITC anti-mouse CD8a, FITC anti-mouse NK-1.1, FITC anti-mouse Ly-6G/Ly-6C (Gr-1), allophycocyanin (APC) anti-mouse CD45, APC/cyanine 7 (Cy7) anti-mouse/human CD44, PerCP/Cy5.5 anti-mouse CD90.2, phycoerythrin (PE) anti-mouse CD278 (inducible costimulatory molecule), APC anti-mouse CD41, PE/Cy7 anti-mouse CD62P, APC anti-human CD61, anti-mouse CD16/32, PE/Cy7 anti-mouse CD45, and PE anti-mouse Siglec F were all obtained from BioLegend (San Diego, Calif).
Liu T., Barrett N.A., Nagai J., Lai J., Feng C, & Boyce J.A. (2020). Leukotriene D4 paradoxically limits LTC4-driven platelet activation and lung immunopathology. The Journal of allergy and clinical immunology, 148(1), 195-208.e5.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!