Mice were fasted for 16 h prior to the collection of blood samples. Blood was collected from the abdominal aorta. Serum TG levels were determined by using Triglyceride Test Wako (Wako Pure Chemical, Osaka Japan), and insulin levels were measured by using ELISA kits (SHIBAYAGI, Gunma, Japan), according to the manufacturer’s instructions. Serum TG, NEFA, total cholesterol, low-density lipoprotein (LDL), and high-density lipoprotein (HDL) levels were determined by using L-Type TG M test, NEFA-C, Cholesterol M, L-Type LDL-C, and L-Type HDL-C Kits (Wako Pure Chemical) according to the manufacturer’s instructions.
L type tg m test
Manufactured by Fujifilm
Sourced in Japan
The L-Type TG M test is a laboratory equipment product from Fujifilm. It is designed to perform specific testing and analysis functions. The core function of this product is to provide accurate and reliable test results for users in a laboratory setting. No further details or interpretation on the intended use of this product can be provided in an unbiased and factual manner.
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3 protocols using l type tg m test
1
Serum Lipid Profile Analysis in Mice
2
Comprehensive Metabolic Profiling of Mice
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Blood samples were collected from mice by cardiocentesis. HbA1c levels were measured in whole blood using CinQ HbA1c (ARKRAY). After centrifugation of the blood at 2000 rpm for 20 min at 4°C, the plasma fraction was collected. Plasma samples were used to determine the levels of blood urea nitrogen (UN-S SEIKEN kit; Denka Co., Ltd.), triglycerides (L-type TG M test; Wako), alanine aminotransferase (L-type ALT J2 test; Wako), aspartate aminotransferase (L-type AST J2 test; Wako), total cholesterol (L-type CHO M test; Wako), high-density lipoprotein cholesterol (Cholestest N HDL; SEKISUI Medical Co., Ltd.), low-density lipoprotein cholesterol (Cholestest LDL; SEKISUI Medical Co., Ltd.), creatinine (L-type CRE M test; Wako), non-esterified fatty acid (NEFA-HR; Wako), total ketone bodies (Auto Wako T-KB; Wako) using the Hitachi 7180 automatic analyzer (Hitachi, Tokyo, Japan). Blood insulin levels were analyzed using a mouse insulin ELISA kit (Morinaga Institute of Biological Science).
3
Quantitative Liver Lipid Extraction
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Liver lipid extraction was conducted as previously described with minor modifications (25 (link)). Briefly, ∼100 mg of liver was thawed, minced, and weighed in a glass tube, and 3 mL CHCl3:MeOH (2:1) was added to the samples to extract lipid at room temperature overnight. The samples were further washed with CHCl3:MeOH (2:1) twice, and the combined solvent extracts were dried at 60°C. Three milliliters of CHCl3:MeOH (2:1) and 0.6 mL 0.05% H2SO4 were added into tubes, and the phases were separated by 2,000 revolutions per minute centrifugation for 15 min. An aliquot of the bottom phase was dried down. The lipid extraction was dissolved in 2% Triton X-100 and used for TG quantification using a L-Type TG M test (Wako Chemicals). The data were normalized to liver weight.
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