Black phosphorus (BP) crystals were purchased from XFNANO (Nanjing, China). C60 (99.6%) was acquired from FUNANO (Xiamen, China). Isopropanol (IPA) and absolute ethanol were obtained from Sinopharm Chemical Reagent Co. Ltd (Shanghai, China). All the chemicals were used directly without further purification. p-Phthalic acid (PTA) was purchased from ShangHai YuanYe Biotechnology (Shanghai, China). Rhodamine B (technical grade) was obtained from INNOCHEM (Beijing, China). PBS and fetal bovine serum (FBS) were acquired from Thermo-Fisher (USA). Fluorescein diacetate (FDA), propidium iodide (PI) and cell counting kit-8 (CCK-8) assays were purchased from Sigma-Aldrich (USA). DCFH-DA and JC-1 were obtained from Beyotime Company (China).
Isopropanol ipa
Manufactured by Sinopharm
Sourced in China
Isopropanol (IPA) is a clear, colorless, and flammable chemical compound commonly used in laboratory settings. It serves as a solvent, disinfectant, and cleaning agent. Isopropanol has a distinctive odor and is miscible with water, making it a versatile tool for various applications in scientific research and experimentation.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Lead iodide pbi2, by Xi'an Yuri Solar Co. (3 mentions)
Litfsi, by Xi'an Yuri Solar Co. (3 mentions)
Methylammonium chloride macl, by Xi'an Yuri Solar Co. (3 mentions)
Spiro ometad, by Xi'an Yuri Solar Co. (3 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (2 mentions)
Dcfh da, by Beyotime (1 mentions)
Absolute ethanol, by Sinopharm (1 mentions)
Dimethyl sulfoxide (dmso), by J&K Scientific (1 mentions)
N n dimethylformamide dmf, by J&K Scientific (1 mentions)
Tetrabutylammonium hydroxide, by Merck Group (1 mentions)
Propidium iodide, by Thermo Fisher Scientific (1 mentions)
Dcfh da, by Yuanye Bio-Technology (1 mentions)
Bp crystals, by XFNANO (1 mentions)
N n dimethylformamide (dmf), by Sinopharm (1 mentions)
Calcein am, by Thermo Fisher Scientific (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Cck 8, by Beyotime (1 mentions)
4t1 cells, by National Collection of Authenticated Cell Cultures (1 mentions)
Fetal bovine serum (fbs), by National Collection of Authenticated Cell Cultures (1 mentions)
7 protocols using isopropanol ipa
1
Synthesis and Characterization of BP-C60 Nanocomposites
2
Synthesis of Perovskite Solar Cell Materials
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The SnO2 colloidal dispersion in H2O (tin (IV) oxide, 15 wt%) was purchased from Alfa Aesar. The lead iodide (PbI2) (> 99.99%), FAI (≥ 99.5%), MAI (≥ 99.5%), methylammonium chloride (MACl) (> 99.5%), spiro-OMeTAD (≥ 99.8%), LiTFSI (> 99%), TBP (> 96%) and PEAI (≥ 99.5%) were purchased from Xi’an Polymer Light Technology Corp. (China). The 1,4-DITFB (97%) was obtained from Macklin. The N,N-dimethylformamide (DMF, 99.9%) and dimethyl sulfoxide (DMSO, 99%) were obtained from J&K Scientific. The isopropanol (IPA, ≥ 99.7%) was purchased from Sinopharm Chemical Reagent Co., Ltd.
3
Nanomaterials Synthesis and Biological Evaluation
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BP crystals were purchased from XFNANO (Nanjing, China). 4‐Azidobenzoin acid (97%), DCC, DMAP, sodium hydroxide (NaOH), and tetrabutylammonium hydroxide (TBAH, 40 wt% in water) were obtained from Sigma Aldrich. C60 (99.6%) was purchased from FUNANO (Xiamen, China). DMF and isopropanol (IPA) were obtained from Sinopharm Chemical Reagent Co. Ltd (Shanghai, China). All chemicals were used directly without further purification. DCFH‐DA and PTA were purchased from ShangHai YuanYe Biotechnology (Shanghai, China). CCK‐8 was obtained from Beyotime Institute of Biotechnology (Jiangsu, China). PBS (10×) saline was purchased from Beyotime Institute of Biotechnology (Jiangsu, China) and diluted by tenfold prior to use. Calcein AM, and propidium iodide (PI) were obtained from Life‐Technologies (ThermoFisher Scientific, USA). FBS was obtained from Shanghai ExCell Biology, Inc. (Shanghai, China). Trypsin (with EDTA, 0.25%) and modified DMEM were purchased from Hyclone. The 4T1 cells were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and cultured in DMEM including 10% FBS at 37 °C in an incubator under a humidified atmosphere condition containing 5% CO2.
4
Biomaterial Synthesis and Cellular Uptake
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GA (purity: 99%) was obtained from Feiyu Biological Co., Ltd (Jiangsu, China). Boron powder, purchased from Maclin (Shanghai, China), was stored in an argon glove box. Isopropanol (IPA) and ethanol (anhydrous grade) were obtained from Sinopharm Chemical Reagents Co., Ltd. (Shanghai, China). PEG-NH2 (MW = 2000) was provided by Xi'an Regal Biotech Co., Ltd. Ultrapure water (18.25 MΩ.cm, 25 °C) was used to prepare all solutions. Phosphate-buffered saline (PBS, pH 7.4), fetal bovine serum (FBS), Dulbecco's modified eagle medium (DMEM), and trypsin-ethylenediaminetetraacetic acid (Trypsin-EDTA) were obtained from Thermo Fisher Scientific Inc. (USA). Cell counting kit-8 (CCK8) was purchased from Dojindo Laboratories (Japan). Cy5.5-PEG-NH2 and FITC-PEG-NH2 were obtained from Ruixi Biological Co., Ltd. (Shanxi, China). Propidium iodide (PI) and calcein acetoxymethyl ester (calcein-AM) were obtained from Solarbio Science & Technology Co., Ltd. (China). Methyl-β-cyclodextrin (MβCD), chlorpromazine (CPZ), hypertonic sucrose, and 5-(N-ethyl- N-isopropyl)-amiloride (EIPA) were purchased from Sigma Aldrich (St. Louis, MO, USA). 4′,6-diamidino-2-phenylindole (DAPI), Lyso Tracker, ER Tracker, and Mito Tracker were obtained from Molecular Probes Inc. (OR, USA).
5
Synthesis and Characterization of Iodinated Pyridinecarboximidamide
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Iodized 3-pyridinecarboximidamide (3API) was synthesized by dissolving 1 g of pyridecarboximidamide (98%, Damas-beta) and 2.25 g hydroiodic acid (HI, 57 wt.% in water, Aldrich) in 10 mL ethanol followed by stirring at 0 °C for 6 h for crystallization. The obtained 3API crystals was dissolved in ethanol for recrystallization. The resulting product was dried at 50 °C in a vacuum oven for 24 h. 3APBr and 3APCl were obtained via a similar fabrication procedure.
Formamidinium iodide (FAI), Pb (II) iodide (PbI2), and spiro-OMeTAD were procured from Advanced Election Technology Co., Ltd. Methylammonium chloride (MACl), Poly[bis(4-phenyl)(2,4,6-trimethylphenyl)amine]) (PTAA) and phenylethylammonium iodide (PEAI) were obtained from Xi’an Polymer Light Technology Co. Chlorobenzene (CB) (anhydrous, 99.8%), Toluene (anhydrous, 99.8%) and isopropanol (IPA) (anhydrous, 99.5%) were purchased from Sinopharm Chemical Reagent Co., Ltd. N,N-dimethylformamide (DMF) (anhydrous, 99.8%) and dimethyl sulfoxide (DMSO) (anhydrous, ≥99.9%) were obtained from Alfa Aesar Inc. 4-tert-butylpyridine (t-BP, 99%), bis(trifluoromethane)sulfonamide lithium salt (Li-TFSI, 99.95% trace metals basis), HI solution (55–58 wt.% in water), HBr solution (48 wt.% in water), and HCl (37 wt.% in water), were purchased from Aladdin.
Formamidinium iodide (FAI), Pb (II) iodide (PbI2), and spiro-OMeTAD were procured from Advanced Election Technology Co., Ltd. Methylammonium chloride (MACl), Poly[bis(4-phenyl)(2,4,6-trimethylphenyl)amine]) (PTAA) and phenylethylammonium iodide (PEAI) were obtained from Xi’an Polymer Light Technology Co. Chlorobenzene (CB) (anhydrous, 99.8%), Toluene (anhydrous, 99.8%) and isopropanol (IPA) (anhydrous, 99.5%) were purchased from Sinopharm Chemical Reagent Co., Ltd. N,N-dimethylformamide (DMF) (anhydrous, 99.8%) and dimethyl sulfoxide (DMSO) (anhydrous, ≥99.9%) were obtained from Alfa Aesar Inc. 4-tert-butylpyridine (t-BP, 99%), bis(trifluoromethane)sulfonamide lithium salt (Li-TFSI, 99.95% trace metals basis), HI solution (55–58 wt.% in water), HBr solution (48 wt.% in water), and HCl (37 wt.% in water), were purchased from Aladdin.
6
Quantitative Analysis of Sulforaphane in Cruciferous Vegetables
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Ferric chloride hexahydrate (FeCl3⋅6H2O), ferrous sulfate heptahydrate (FeSO4·7H2O), polyethylene glycol 6000 (PEG), acetic acid (HAc), acetonitrile (ACN, HPLC grade), methanol (MeOH, HPLC grade), ammonium acetate (HPLC grade), dichloromethane (DCM), acetone (AC), and isopropanol (IPA) were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Dimethyl sulfoxide (DMSO), ammonia (28%), and ethanol (EtOH) were purchased from Tianjin Huihang Chemical Technology Co., Ltd. (Tianjin, China). DL-sulforaphane standard (SFN, purity of 97%), 1,3,5-benzenetricarboxaldehyde (Tb), benzidine (Bd), 1,3,5-tris (4-aminophenyl) benzene (TAPB), and m-phthalaldehyde (MPA) were provided by Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Water was purified by a Milli-Q Advantage A10 system (USA). All the reagents were analytical grade at least.
Considering the wide variation in SFN content in different cruciferous vegetables, 10 cruciferous vegetable samples including broccoli, cauliflower, cabbage, Brassica campestris, Chinese cabbage, carrot, red radish, white radish, green turnip, and pak choi (Brassica chinensis L.) were purchased from a local supermarket in Beijing, China. All vegetable samples were homogenized by a blender before sample pretreatment.
Considering the wide variation in SFN content in different cruciferous vegetables, 10 cruciferous vegetable samples including broccoli, cauliflower, cabbage, Brassica campestris, Chinese cabbage, carrot, red radish, white radish, green turnip, and pak choi (Brassica chinensis L.) were purchased from a local supermarket in Beijing, China. All vegetable samples were homogenized by a blender before sample pretreatment.
7
Synthesis of Bi-based Electrocatalyst
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Following reagents were used: BiCl3, ethanolamine and acetaminophen (Aladdin Biochemical Technology Co., Ltd., Shanghai, China), Graphite powder and NH4VO3 (Bodi Chemical Co., Ltd., Tianjin, China), Bi(NO3)3·5H2O and ethylene diamine tetraacetic acid disodium salt (EDTA-2Na) (Tianjin Damao Chemical Reagent Co. Inc., Tianjin, China), formic acid, isopropanol (IPA) and p-Benzoquinone (Sinopharm Chemical Reagents Co., Ltd., Shanghai, China). All chemicals were used as received without further purification. Deionized water was used throughout the experiment.
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