Urine samples were collected overnight (approximately 16 h) using a cage pan, once during the acclimation period, on days 31 and 59. Animals were not fasted prior to blood collection. The collected urine samples were centrifuged (1500 rpm, 5 min at room temperature) for urine chemistry and urine sediment analysis. Urinalysis parameters were measured by reading the scales on the urine collection tubes of Combur 10 TM urine sticks (Roche) and Cobas U411 urine analyzer (Roche) and then examined visually or under a microscope. These parameters included volume (VOL), color (COL)/clarity (CLA), pH, specific gravity (SG), bilirubin (BILU), protein (PRO), urobilinogen (UBG), nitrite (NIT), glucose (GLUU), erythrocytes (ERY), ketones (KETU), leukocytes (LEU), and urine sediments (urine cast, epithelial cells, white blood cells, and red blood cells). Urine potassium (UK), chloride (UCl), and sodium (Una) levels were measured using a Toshiba 2000FR NEO chemistry analyzer.
Combur 10 urine sticks
Manufactured by Roche
Sourced in Germany
Combur 10 TM urine sticks are a diagnostic laboratory test designed to analyze various parameters in urine samples. The device combines ten different reagent pads on a single test strip, allowing for the simultaneous detection and measurement of multiple urinary analytes.
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Lab products found in correlation
3 protocols using combur 10 urine sticks
1
Comprehensive Urine Analysis Protocol
2
Overnight Urine Analysis in Fasted Animals
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Urine was collected overnight (∼ 16 h period) the day before terminal sacrifice. Animals were housed in individual metabolic cages during this period. Water was available ad libitum but animals were fasted. Urine volume was measured and the following parameters were analyzed using a Cobas U411 uring analyzer (Roche, Germany) and Combur 10 T M urine sticks (Roche, Germany): pH, specific gravity, bilirubin, protein, urobilinogen, nitrite, glucose, erythrocytes and ketones. Urine cast, epithelial cells, white blood cells and red blood cells were examined by microscopy of urine micro sediments.
3
Comprehensive Urinalysis Protocol
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Urine samples were collected at approximately 3–4 h within 1 week prior to necropsy. The following parameters were measured during urinalysis: color, clarity, glucose, pH, erythrocyte, specific gravity, ketone, bilirubin, leukocytes, protein, nitrite, and urobilinogen. All parameters except for urine color were analyzed using Combur 10 TM urine sticks (Roche, Germany) and a Cobas U411 urine analyzer (Roche, Germany).
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