Lambda 35 uv vis spectrometer

Manufactured by PerkinElmer

Sourced in United States, United Kingdom

The Lambda 35 UV/VIS Spectrometer is a laboratory instrument designed to measure the absorption or transmission of light in the ultraviolet and visible light spectrum. It is capable of performing qualitative and quantitative analysis of samples by detecting the interaction of light with materials.

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Close spelling variants of this product

Lambda 35 UV/VIS Lambda 35 UV Lambda 35 spectrometer UV-vis spectrometer Lambda 35 Spectrometers

119 protocols using lambda 35 uv vis spectrometer

Dye Solubilization and CPC/CR Stoichiometry

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The solubilization experiments were performed by adding an excess of the crystalline dye methyl yellow (MY) to aqueous solutions of CR (2.86 mM), CPC (1 mM), CR-CPC 4/1 (C(CPC) = 0.976 mM, C(CR) = 0.244 mM), and CPC/CR 1/4 (C(CPC) = 0.714 mM, C(CR) = 2.86 mM) mixtures. The solutions were mixed at 20 1C for 4 h at a rate of 360 rpm. They were then centrifuged (rate 6 krpm, 10 min, centrifuge Eva-20 (Hettich Zentrifugen, Germany)) to separate non-solubilized MY. The UV Vis spectra of the obtained solutions were registered on a Lambda 35 UV-Vis Spectrometer (Perkin-Elmer Instruments, USA) using quartz cells with an optical path of 0.1 cm.
The study of stoichiometry of CPC/CR by UV/Vis method A number of CPC/CR solutions with constant concentrations of CPC (0.1 and 0.5 mM) and different CR/CPC molar ratios (from 10/1 to 1/8) were prepared. The absorbance at 500 nm was measured with a Lambda 35 UV-Vis Spectrometer (Perkin-Elmer Instruments, USA) using quartz cells with an optical path of 0.2 cm.

Morozova J.E., Syakaev V.V., Shalaeva Y.V., Ermakova A.M., Nizameev I.R., Kadirov M.K., Voloshina A.D., Zobov V.V., Antipin I.S, & Konovalov A.I. (2017). Unusual nanosized associates of carboxy-calix[4]resorcinarene and cetylpyridinium chloride: the macrocycle as a glue for surfactant micelles. Soft matter, 13(10).

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Characterization and Uptake of Folate-Functionalized Nanoparticles

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The morphology, size, zeta potential, and stability of the FA-ALNBs were detected by optical microscopy (Olympus Corporation, Tokyo, Japan), transmission electron microscopy (TEM, SU8010; Hitachi Ltd., Tokyo, Japan), and DLS analysis using a Zetasizer Nano ZS system (Malvern Instruments, Malvern, UK). The cellular uptake of the FA-ALNBs was evaluated by confocal laser scanning microscopy (LEXT OLS4100; Olympus) and flow cytometry (FACSCanto II; BD, Bedford, MA, USA). The absorption spectra of the nanoparticles were detected on a Lambda 35 UV–vis spectrometer (PerkinElmer Inc., Waltham, MA, USA).

Gao S., Cheng X, & Li J. (2019). Lipid nanobubbles as an ultrasound-triggered artesunate delivery system for imaging-guided, tumor-targeted chemotherapy. OncoTargets and therapy, 12, 1841-1850.

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Comprehensive Characterization of Organic Compounds

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¹H NMR and ¹³C NMR spectra were recorded with
a Bruker AVANCE NMR spectrometer. Elemental analysis was performed
using a Bio-Rad elemental analysis system. MALDI-TOF mass spectra
were performed on an AXIMA CFR MS apparatus (COMPACT). Thermal gravimetric
analysis (TGA) and differential scanning calorimetry (DSC) were performed
with a PerkinElmer-TGA 7 and PerkinElmer-DSC 7 instrument, respectively,
under a nitrogen atmosphere at a heating rate of 10 °C/min. UV–vis
absorption and PL spectra were recorded on a PerkinElmer LAMBDA 35
UV–vis spectrometer and PerkinElmer LS 50B spectrofluorometer,
respectively. The PLQYs were measured using a quantum yield measurement
system (C10027, Hamamatsu Photonics) excited at 360 nm. The transient
PL spectra were measured by a HORIBA Jobin Yvon Fluorolog-3 spectrofluorometer.
Also, the prompt and delay lifetimes were estimated according to a
monoexponential and tri-exponential fittings, respectively. CV curves
were recorded on an EG&G 283 Princeton Applied Research potentiostat/galvanostat
system. Ferrocene was used as the reference and n-Bu₄NClO₄ was used as the supporting electrolyte.
The HOMO and LUMO energy levels were calculated according to the equation
HOMO = −e[E_onset,ox + 4.8] V, LUMO = HOMO + E_g, where E_onset,ox was the onset value of the first oxidation
potential, and E_g was the optical band
gap estimated from the absorption onset.

Rao J., Zhao C., Wang Y., Bai K., Wang S., Ding J, & Wang L. (2019). Achieving Deep-Blue Thermally Activated Delayed Fluorescence in Nondoped Organic Light-Emitting Diodes through a Spiro-Blocking Strategy. ACS Omega, 4(1), 1861-1867.

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Thermoresponsive Polymer Optical Transmittance

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Temperature-dependent optical transmittance was examined on a Perkin-Elmer Lambda 35 UV–vis spectrometer (Perkin-Elmer, Waltham, MA, USA). A thermostatically controlled cuvette was employed, and the heating rate was 0.2 °C/min. The concentration of the polymer solution was 1 mg/mL using phosphate buffer saline (PBS, pH 7.4, 10 mM) as the solvent.

Tu X., Meng C., Liu Z., Sun L., Zhang X., Zhang M., Sun M., Ma L., Liu M, & Wei H. (2017). Synthesis and Phase Transition of Poly(N-isopropylacrylamide)-Based Thermo-Sensitive Cyclic Brush Polymer. Polymers, 9(7), 301.

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Comprehensive Materials Characterization Protocol

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SEM results were collected with a JEOL microscope (model JSM-6700F). TEM images were collected from a JEOL microscope (model JEM-1400). EDS results were also obtained from the same model JEOL microscope with SEM tests. XRD patterns were collected with a D8 focus diffractometer (Bruker AXS, Germany). FTIR tests were carried out on a PerkinElmer 580B spectrophotometer made in the United States. XPS data were obtained from an X-ray photoelectron spectroscope (ESCALAB 250, ThermoFisher Scientific). The electrochemical tests (EIS and i–t) were tested on a Zahner electrochemical workstation (model Zennium PP211) with a three-electrode system. ZSimpWin software was used to fit the parameters for EIS data. PL spectra were obtained from a LS-45/55 spectrometer (PerkinElmer). UV–vis experiments were performed on a Lambda 35 UV–vis spectrometer (PerkinElmer).

Pang X., Zhang X., Gao K., Wan S., Cui C., Li L., Si H., Tang B, & Tan W. (2019). Visible Light-Driven Self-Powered Device Based on a Straddling Nano-Heterojunction and Bio-Application for the Quantitation of Exosomal RNA. ACS nano, 13(2), 1817-1827.

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Tablet Dissolution and UV Analysis

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From each formulation, three tablets were randomly selected and a mortar and pestle were used to convert tablets into powder form. One hundred milligrams of the crushed tablets of each batch was weighed, added to a volumetric flask, and the volume brought up to 100 mL using distilled water. The solution was passed through filter paper and absorbance of the solution was determined at 233 nm using ultraviolet spectrophotometer (Lambda 35 UV Vis Spectrometer; PerkinElmer Inc., Waltham, MA, USA). The percentage of the drug content was calculated.21 (link)

Razavi M., Karimian H., Yeong C.H., Chung L.Y., Nyamathulla S, & Noordin M.I. (2015). Gamma scintigraphic evaluation of floating gastroretentive tablets of metformin HCl using a combination of three natural polymers in rabbits. Drug Design, Development and Therapy, 9, 4373-4386.

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Evaluation of Metformin HCl Floating Matrix Tablets

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The release of the metformin HCl from the floating matrix tablets was evaluated using a United States Pharmacopeia dissolution apparatus II, paddle type (Copley Scientific Limited, Nottingham, UK). A tablet was placed inside the dissolution vessel containing 900 mL of 0.1 N HCl (pH 1.2) at 37°C±0.5°C and stirred at a speed of 50 rpm. Five milliliters of sample was withdrawn at 0, 0.5, 1, 1.5, 2, 3, 4, 5, 6, 7, 8, 9, 10 and 12 hour time intervals and each time the same amount of fresh media was used to maintain a constant volume. The amount of drug release was determined using a spectrophotometer (Lambda 35 UV–Vis Spectrometer; PerkinElmer Inc.) at a wavelength of 233 nm against 0.1 N HCl as a blank. The amount of drug in each sample was calculated using a standard calibration curve. The in vitro drug release was carried out in triplicate for each batch of tablets. Release profiles of the designed formulations were compared with the commercial formulation (C-ER). Similarity and difference factors were calculated using appropriate formulas. The in vitro release data were analyzed using mathematical models representing: 1) first order; 2) zero order; 3) Hixson− Crowell; and 4) Higuchi’s equations.15

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UV-Vis Spectroscopy Characterization Protocol

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UV-vis spectra were recorded by Lambda 35 UV-vis spectrometer (Perkin-Elmer, USA) at 25°C with a 1.0 cm path length quartz cell at wavelength from 250 to 375 nm with resolution of 1 nm. All UV-vis experiments were repeated three times and averaged with standard deviation (mean ± SD).

Xu Z.X., Zhang Q., Ma G.L., Chen C.H., He Y.M., Xu L.H., Zhang Y., Zhou G.R., Li Z.H., Yang H.J, & Zhou P. (2016). Influence of Aluminium and EGCG on Fibrillation and Aggregation of Human Islet Amyloid Polypeptide. Journal of Diabetes Research, 2016, 1867059.

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NMR and Mass Spectrometry Characterization Protocol

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¹H NMR and ¹³C NMR spectra were collected via a 400
MHz Varian Unity Inova NMR spectrophotometer. ¹H NMR and
¹³C NMR spectra were recorded in CDCl₃ solutions.
Chemical shifts (δ) were given in ppm relative to
solvent residual peaks (¹H, δ 7.26 for
CDCl₃; ¹³C, δ 77.3 for
CDCl₃) as internal standards. High-resolution mass spectrometry
data (HRMS) of the intermediates and fluorescent probes were measured with fast
atom bombardment (FAB) ionization mass spectrometer, double focusing magnetic
mass spectrometer or matrix assisted laser desorption/ionization time-of-flight
mass spectrometer. Absorption spectra were taken on a Perkin Elmer Lambda 35
UV/vis spectrometer. Fluorescence spectra were recorded on a Jobin Yvon
Fluoromax-4 spectrofluorometer.

Zhang S., Adhikari R., Fang M., Dorh N., Li C., Jaishi M., Zhang J., Tiwari A., Pati R., Luo F.T, & Liu H. (2016). Near-Infrared Fluorescent Probes with Large Stokes Shifts for Sensing Zn(II) Ions in Living Cells. ACS sensors, 1(12), 1408-1415.

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Schlenk Techniques for Inorganic Synthesis

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When required, manipulations were performed using
standard Schlenk techniques under dry nitrogen or in an MBraun glovebox.
Nitrogen was purified by passing it through columns of supported P₂O₅ with moisture indicator and activated 4 Å
molecular sieves. Anhydrous solvents were freshly distilled from appropriate
drying agents. ¹H and ¹³C{¹H} spectra
were recorded using a Bruker Avance DPX-300 spectrometer. ¹H NMR spectra (300.13 MHz) were referenced to the residual protons
of the deuterated solvent used. ¹³C{¹H} NMR
spectra (75.47 MHz) were referenced internally to the D-coupled ¹³C resonances of the NMR solvent. Elemental analyses were
carried out at London Metropolitan University. UV–vis absorption
spectra were recorded using a PerkinElmer Lambda 35 UV–vis
spectrometer. Excitation and emission spectra were measured in a (TCSPC)
Horiba Jobin Yvon FluoroLog spectrofluorometer. Compounds 1, 5, 6, and 7 were synthesized
following reported procedures.^{19 (link),20 (link)}

Bertrand B., Fernandez-Cestau J., Angulo J., Cominetti M.M., Waller Z.A., Searcey M., O’Connell M.A, & Bochmann M. (2017). Cytotoxicity of Pyrazine-Based Cyclometalated (C^Npz^C)Au(III) Carbene Complexes: Impact of the Nature of the Ancillary Ligand on the Biological Properties. Inorganic Chemistry, 56(10), 5728-5740.

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