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C1 crl 2097 fibroblasts

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C1 (CRL-2097) fibroblasts are a cell line derived from normal human skin. Fibroblasts are a type of cell that play a crucial role in the production and maintenance of the extracellular matrix in connective tissue.

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Lab products found in correlation

Endothelial growth factor, by Thermo Fisher Scientific (2 mentions) Basic fibroblast growth factor (bfgf), by Thermo Fisher Scientific (2 mentions) Stempro, by Thermo Fisher Scientific (2 mentions) Pen strep, by Thermo Fisher Scientific (2 mentions) Knockout dmem f 12, by Thermo Fisher Scientific (2 mentions) Glutamax, by Thermo Fisher Scientific (2 mentions)

3 protocols using c1 crl 2097 fibroblasts

1

Derivation and Culture of NPCs from iPSCs

Cited 2 times
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IPSC-derived neural progenitor cells (NPC) were kindly provided by Professor Hongjun Song’s lab within our joint NIH NCATS project (Pamies et al., 2016 (link)). NPCs were derived from C1 (CRL-2097) fibroblasts purchased from ATCC. Differentiation from iPSC to NPC has been previously described (Wen et al., 2014 (link)). NPCs were cultured in poly-lornithine and laminin-coated 175 cm2 flasks in NPC medium (KnockOut DMEM/F12 [Gibco] supplemented with 2% Stempro® [Gibco], 1% Glutamax [Gibco], 1% Pen-strep [Gibco], 20 ng/ml endothelial growth factor [Gibco], and 20 ng/ml basic fibroblast growth factor [Gibco]). Half of the media was exchanged every day. Cultures were maintained at 37 °C in a humidified atmosphere of 5.0% CO2. Passages 17–24 were used for neural differentiation.
Pamies D., Wiersma D., Katt M.E., Zhao L., Burtscher J., Harris G., Smirnova L., Searson P.C., Hartung T, & Hogberg H.T. (2022). Human IPSC 3D brain model as a tool to study chemical-induced dopaminergic neuronal toxicity. Neurobiology of disease, 169, 105719.
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2

Expansion and Differentiation of Neural Progenitor Cells

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
NPC were kindly provided by Professor Hongjun Song’s lab within our joint NIH NCATS project (Pamies et al., 2017 (link)). NPCs were derived from C1 (CRL-2097) fibroblasts purchased from ATCC. Differentiation from iPSC to NPC has been previously described (Wen et al., 2014 (link)). NPCs were expanded in poly-l-ornithine and laminin-coated 175 mm2 flasks in NPC expanding medium (KnockOut DMEM/F12, Glutamax, EGF and bFGF). Half of the media was changed every day. Cultures were maintained at 37 °C in an atmosphere of 5% CO2. NPC from passage 15 to 25 were used for this study.
Pamies D., Block K., Lau P., Gribaldo L., Pardo C.A., Barreras P., Smirnova L., Wiersma D., Zhao L., Harris G., Hartung T, & Hogberg H.T. (2018). Rotenone exerts developmental neurotoxicity in a human brain spheroid model. Toxicology and applied pharmacology, 354, 101-114.
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3

Derivation and Culture of NPCs from iPSCs

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
IPSC-derived neural progenitor cells (NPC) were kindly provided by Professor Hongjun Song’s lab within our joint NIH NCATS project (Pamies et al., 2016 (link)). NPCs were derived from C1 (CRL-2097) fibroblasts purchased from ATCC. Differentiation from iPSC to NPC has been previously described (Wen et al., 2014 (link)). NPCs were cultured in poly-lornithine and laminin-coated 175 cm2 flasks in NPC medium (KnockOut DMEM/F12 [Gibco] supplemented with 2% Stempro® [Gibco], 1% Glutamax [Gibco], 1% Pen-strep [Gibco], 20 ng/ml endothelial growth factor [Gibco], and 20 ng/ml basic fibroblast growth factor [Gibco]). Half of the media was exchanged every day. Cultures were maintained at 37 °C in a humidified atmosphere of 5.0% CO2. Passages 17–24 were used for neural differentiation.
Pamies D., Wiersma D., Katt M.E., Zhao L., Burtscher J., Harris G., Smirnova L., Searson P.C., Hartung T, & Hogberg H.T. (2022). Human IPSC 3D brain model as a tool to study chemical-induced dopaminergic neuronal toxicity. Neurobiology of disease, 169, 105719.
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