Human mmp 9 platinum elisa kit

Circulating levels of Annexin-A3 (ANX3), Defensin (DEFA1), proteinase-3 (PR3), and Matrix Metalloproteinase-9 (MMP9) proteins were assessed in plasma samples from subjects using the ELISA method using the following kits: Human Annexin A3 ELISA kit (DL-ANX3-Hu, Donglin, Wuxi, China), Human Defensin HNP1-3 and Proteinase 3 (HK317 and HK384, respectively, Hycult Biotech, Uden, The Netherlands), and the Human MMP-9 Platinum ELISA kit (BM2016/2, eBioscience, San Diego, CA, USA). The assays were performed according to the manufacturer’s instructions.

Levels of SDF-1α, VEGF, MMP-9, IL-8 and IL-17 in serum samples were measured by commercial quantitative colorimetric sandwich enzyme-linked immunosorbent assay (Human CXCL12/SDF-1α Quantikine ELISA Kit, Human VEGF Quantikine ELISA Kit and Human IL-17 DuoSet ELISA Kit, all from R&D Systems, Minneapolis, MN, USA; Human IL-8/NAP-1 INSTANT ELISA Kit and Human MMP-9 Platinum ELISA Kit, both from eBioscience, San Diego, CA, USA) according to the manufacturer’s instructions. The detection range was 0.156–10.0 ng/ml for SDF-1α, 31.2–2,000 pg/ml for VEGF, 0.23–15.0 ng/ml for MMP-9, 15.6–1,000 pg/ml for IL-8 and 15.6–1,000 pg/ml for IL-17. Serum samples were diluted 1:250 for the MMP-9 assay. Concentrations were calculated using a standard curve generated with specific standards provided by the manufacturer. Each sample was measured in duplicate.