Anti il 6 antibody ab32530

ARPE-19 cells (CRL-2302), THP-1 cells (TIB-202), and RPMI-1640 medium (30–2001) were purchased from the American Type Culture Collection (ATCC) (Manassas, VA). Human primary retinal pigment epithelial cells (hRPE) (194987) were obtained from Lonza (Walkersville, MD). Dulbecco’s Modified Eagle Medium (DMEM/F-12) (11330-057), heat-inactivated fetal bovine serum (HI-FBS) (10438-026) and Penicillin-Streptomycin (15140122) were purchased from Life Technologies (Grand Island, NY). HP X-tremeGene transfection agent (06366244001) was obtained from Roche (Indianapolis, IN), recombinant human IL-1α (200-LA-010) from R&D Systems (Minneapolis, MN), and Bay 11–7082 inhibitor (196870) from EMD Millipore (Billerica, MA). Anti-IL-18 antibody (ab137664), anti-IL-6 antibody (ab32530), and anti-TATA binding protein (TBP) (51841) were obtained from Abcam (Cambridge, MA). Anti-IL-1β antibody (MAB201), anti-IL-8 antibody (MAB208), and anti-STING (MAB7169) were purchased from R&D Systems. Anti-caspase-1 (sc-515) was purchased from Santa Cruz Biotechnology (Dallas, TX). Anti-β-tubulin antibody (2128), anti-A20 (5630) and HRP-linked secondary antibodies (7074S, 7076S) were obtained from Cell Signaling Technology (Danvers, MA). ELISA kits for IL-1β (DLB50), IL-18 (7620) and IL-8 (D8000C) were purchased from R&D. Taq Polymerase (M0267S) was purchased from New England Biolabs (Ipswich, MA).

The human RPE cell line ARPE-19 was purchased from ATCC (Manassas, VA, US). DMEM/F-12, HEPES medium (#11330-057) was purchased from Life Technologies (Grand Island, NY, US). FBS (#SCRR-30-2020) was obtained from ATCC. Penicillin-Streptomycin (#15140122) was obtained from Life Technologies. Recombinant human IL-1α (200-LA-002) was obtained from R&D Systems (Minneapolis, MN, US). Cholesterol crystals (#C8667) were purchased from Sigma-Aldrich (St. Louis, MO, US). Bay 11-7082 inhibitor (#196870) was obtained from EMD Millipore. Anti-IL-18 antibody (#ab137664), anti-IL-6 antibody (#ab32530), and anti-β-actin antibody (ab8227) were obtained from Abcam (Cambridge, MA, US). Anti-IL-1β antibody (#MAB201) and anti-IL-8 antibody (#MAB208) were purchased from R&D Systems. HRP-linked secondary antibodies (7074S, 7076S) were obtained from Cell Signaling Technology (Danvers, MA, US).