For immunofluorescence (IF), the following primary antibodies were used: rabbit anti-EGFP (Invitrogen/A-11122, 1:500), rat anti-BrdU (Serotec/OBT0030G, 1:400), rabbit anti-CST6 (Aviva Systems Biology/ARP53533_P050, 1:100), and chicken anti-β-galactosidase (Abcam/ab9361, 1:400). ISH was performed using an RNAscope Fluorescent Multiplex Kit (Advanced Cell Diagnostics) according to the manufacturer’s instructions. IF was performed on PFA-fixed horizontal whole-mount dorsal skin preparations and ISH on PFA-fixed paraffin-embedded dorsal skin sections (Supplemental Experimental Procedures ).
A 11122
Manufactured by Bio-Rad
The A-11122 is a laboratory instrument designed for centrifugation. It is capable of separating components of a solution based on their density and size.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using a 11122
1
Immunofluorescence and In Situ Hybridization
2
Multiplex Immunohistochemistry Antibody Panel
3
Immunocytochemistry of Neural Progenitors
Check if the same lab product or an alternative is used in the 5 most similar protocols
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NPCs plated on PDL/laminin-coated coverslips were fixed with ice-cold 4% paraformaldehyde (PFA) for 10 minutes. Cells were blocked for 1 hour in PBS containing 10% donkey serum and 0.2% Triton X-100 and then incubated overnight at 4°C with one or more of the following primary antibodies: mouse anti-βIII-tubulin (1:2000, Promega, #G7121, Clone Tuj1), mouse anti-MAP2 (1:2000, Sigma-Aldrich, #M1406), rabbit anti-GFP (1:1000, Invitrogen, # A-11122), and rat anti-BrdU (1:500, AbD Serotec, #OBT0030). On the next day, cells were incubated with Cy3- or DyLight488-conjugated secondary antibodies for 2 hours at room temperature. Nuclei were stained with Hoechst or DAPI, and the cells were mounted onto glass slides for microscopic evaluation.
For BrdU incorporation assay, NPCs were pretreated with 10 μM BrdU for 2 hours before fixation. Cells were sequentially washed with Tris-buffered saline (TBS) and 0.9% saline and then treated with 1M HCl for 30 minutes before going through the above staining procedures.
For BrdU incorporation assay, NPCs were pretreated with 10 μM BrdU for 2 hours before fixation. Cells were sequentially washed with Tris-buffered saline (TBS) and 0.9% saline and then treated with 1M HCl for 30 minutes before going through the above staining procedures.
4
Multiplex Immunohistochemistry Antibody Panel
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Rabbit anti-NPY62 (link) (1:2000, ImmunoStar 22940), Goat anti-AGRP (1:5000, Neuromics GT15023)63 (link), guinea pig anti-RFP24 (link) (1:25000, Covance), rabbit anti-POMC (27–52)55 (link) (1:2000 Phoenix Pharmaceuticals, H02930), rabbit anti-GFP (1:5000, Invitrogen A-11122), sheep anti-GFP (1:3000, AbD Serotec 47451051), rabbit anti-vGlut164 (link) (1:2000, SYSY, 135302), guinea pig anti-vGlut2 (1:2000, SYSY 135404), rabbit anti-vGlut265 (link) (1:1000, SYSY 135402), mouse anti-vGat (1:100, SYSY 131011), rabbit anti-vGat66 (link) (1:4000, Covance), goat anti-vAcht (1:2000, Chemicon ABN100). Fluorophore-conjugated, minimal cross reactivity secondary antibodies were from Jackson Immuno (1:500).
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