The protein expression levels of p‐ATF2, p‐JNK and p‐p38 MAPK were detected on knee joint cartilage using immunohistochemical analyses. Immunohistochemical staining was carried out using the streptavidin‐peroxidase method. The tissue sections were incubated with primary antibodies at 4°C overnight, followed by the biotinylated secondary antibody. The positive expression of p‐ATF2, p‐JNK and p‐p38 MAPK appeared brown. The number of immunoreactive cells in sections was analysed using Image‐Pro Plus 6.0 analysis software (Media Cybernetics, Inc., Silver Spring, MD, USA).
Image pro plus 6.0 analysis
Manufactured by Media Cybernetics
Sourced in United States
Image‐Pro Plus 6.0 is a comprehensive software package designed for advanced image analysis and processing. It provides a suite of tools for image capture, enhancement, measurement, and analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using image pro plus 6.0 analysis
1
Protein Expression Profiling of Knee Cartilage
2
Quantification of Atrial Collagen Fibers
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Sections (5-µm thick) of atrial tissue were stained with H&E and Masson’s staining and then sealed (Con n=10, DM n=11). The areas of the blue-purple collagen fibers and the percentage of the area relative to the entire field of view were assessed using Image-Pro Plus 6.0 analysis software (Media Cybernetics, Inc., Rockville, MD, USA).
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