Harzianic acid was obtained from T. harzianum M10 grown in liquid medium (PDB, HiMedia, Mumbai, India) for 21 days and purified following a previously described protocol with some modifications [25 (link)]. Briefly, culture filtrate was exhaustively extracted with ethyl acetate (EtOAc, Carlo Erba, Cornaredo, Milan, Italy), and the dry residue was resuspended in dichloromethane (DCM, Carlo Erba) and extracted with a 2M solution of sodium hydroxide (NaOH, Carlo Erba). The aqueous phase was acidified at pH = 2 with hydrochloric acid (HCl, Carlo Erba), and harzianic acid was obtained after vacuum filtration and precipitate wash with EtOAc. Harzianic acid identification was achieved by NMR and LC-MS analyses [30 (link),31 (link)].
Sodium hydroxide (naoh)
Manufactured by Carlo Erba
Sourced in Italy, France, Germany, Spain, United States
Sodium hydroxide is a commonly used chemical compound in laboratory settings. It is a white, crystalline solid that is highly soluble in water. Sodium hydroxide's core function is as a strong base, which is used to neutralize acids, adjust pH levels, and perform various chemical reactions.
Automatically generated - may contain errors
Lab products found in correlation
Hydrochloric acid (hcl), by Carlo Erba (15 mentions)
Gallic acid, by Merck Group (5 mentions)
Hydrochloric acid (hcl), by Merck Group (4 mentions)
Acetonitrile, by Carlo Erba (4 mentions)
Ethanol, by Carlo Erba (4 mentions)
Methanol, by Carlo Erba (4 mentions)
Sodium chloride (nacl), by Merck Group (3 mentions)
Quercetin, by Merck Group (3 mentions)
Formic acid, by Carlo Erba (3 mentions)
Sodium carbonate, by Merck Group (3 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (3 mentions)
Sulfuric acid, by Merck Group (3 mentions)
Potassium permanganate, by Merck Group (3 mentions)
Sodium chloride (nacl), by Carlo Erba (3 mentions)
Hydrochloric acid (hcl), by Avantor (3 mentions)
Hydrogen peroxide h2o2, by Merck Group (3 mentions)
Boric acid, by Carlo Erba (2 mentions)
Trolox, by Merck Group (2 mentions)
Acetonitrile, by Merck Group (2 mentions)
Milli q system, by Merck Group (2 mentions)
59 protocols using sodium hydroxide (naoh)
1
Harzianic Acid Purification from Trichoderma
2
Magnetically-Driven Enzymatic Synthesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
L-Phenylalanyl-L-phenylalanine (H-Phe-Phe-OH, 98%, 312.36 g/mol) and N-(9-Fluorenylmethoxycarbonyl)-L-phenylalanine (Fmoc-L-phenylalanine: Fmoc-Phe-OH, 99%, 387.44 g/mol) were purchased from Bachem GmbH (Weil am Rhein, Germany) and used as received. FeCl2·4H2O (198.75 g/mol) and FeCl3 (162.20 g/mol) were purchased from Fluka. NaOH (39.99 g/mol) and CrCl3·6H2O (158.36 g/mol) were purchased from Carlo Erba Reagents (Cornaredo (MI), Italy). NiCl2·6H2O (129.59 g/mol) and CoCl2·6H2O (129.83 g/mol) were obtained from Alfa Aesar. Lipase from Pseudomonas fluorescens (PFL ≥ 20,000 U/mg) was purchased from Sigma-Aldrich (Milan, Italy) and used as received. Ultra-pure water (H2Oup) was obtained using a Zeneer Power I Scholar-UV (Full Tech Instruments, Rome, Italy) apparatus. In this study, the external magnetic field was provided by a commercial neodymium-based magnet possessing 39.270 cm3 volume, magnetization quality of N5, and 1.42–1.47 T of magnetic strength.
3
Quantification of Flavonoids in Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Flavonoids were measured according to the method outlined by Heimler et al. [67 (link)]. An amount of 500 mg of ground samples was homogenized in 2 mL of 80% ethanol and then centrifuged at 15,000 rpm (Z 233 MK-2, Hermle, LaborTechnik GmbH, Wehingen, Germany) for 5 min. The supernatant (300 µL) was added to 45 µL of a 10% AlCl3 (Carlo Erba, Cornaredo, Milan, Italy) solution, 300 µL of a 1M NaOH (Carlo Erba, Cornaredo, Milan, Italy) solution, and 300 µL of deionized water. Samples were read at 510 nm with a UV-Vis spectrophotometer (8453, Agilent, Santa Clara, CA, USA). Quantification was performed with a calibration curve (5–200 µg mL−1) of quercetin (Sigma-Aldrich, Burlington, MA, USA), and the results were expressed as mg of quercetin equivalent on a dry weight basis (mg QE g−1 dw).
4
Antioxidant Activity Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
xanthine oxidase from bovine milk, luminol sodium salt, xanthine, oxypurinol, PBS tabs, Na-EDTA salt, gelatin from bovine skin, penicillin/streptomycin, trypsin-EDTA, Trolox, and 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sodium perborate, boric acid, NaOH, and FeCl2 were from Carlo Erba (Milan, Italy). M200 medium, low serum growth supplements, and fetal bovine serum, RNaseOUT, were purchased from Thermo Fisher Scientific (Waltham, MA, USA). RNeasy Mini Kit was from QIAGEN (Hilden, Germany). Primers for RT-PCR were purchased from IDT (Coralville, IA, USA). Cell counting kit-8 (CCK8) and LDH assay kit were purchased from Dojindo Molecular Technologies (Rockville, MD, USA). SuperScript® III First-Strand Synthesis SuperMix and EXPRESS SYBR® GreenER™ qPCR SuperMix were purchased from Life Technologies (Carlsbad, CA, USA). All the other chemicals and solvents were of the highest analytical grade.
5
Semiconductor Oxides for Cyanide Removal
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Two sources of semiconductor oxides were used: TiO2 Degussa P-25 (80 anatase and 20% rutile) with a surface area of ≈54 m2/g, 0.2031 cm3/g and pore size 0.016 A μm, particle size 0.34 μm and Nb2O5 · 3H2O, donated by CBMM (Brazilian Metallurgy Company) 98% purity, whose characteristics include a surface area of 170 m2/g before some pre-treatment temperature, pore volume of 0.2 cm3/g, and pore size of 2–5 μm (D50). For kinetic experiments, KCN (Panreac) was used as a source of cyanide, HCl (Merck) and NaOH (Carlo Erba) reagent grade to adjust the pH between 9.5 and 12.
6
Functionalization of Ordered Mesoporous Silica
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All reagents used were of analytical grade. Ordered mesoporous silica of the SBA-15 type was purchased from ACS Material Advanced Chemical Supplier (Pasadena, CA, USA). (3-aminopropyl) triethoxysilane (APTES, 99%), N- propyl]aniline, toluene (99.8%), acetonitrile ( > 99.9%) were purchased from Sigma Aldrich (Steinheim, DE). Primary Secondary Amine (PSA, Fig. 1 A Hydrochloric acid (35% w/w, d = 1.187 g/mL) and NaOH ( > 98%) were from Carlo Erba (Milano, IT). NaCl, MgSO 4 •7H 2 O and H 2 SO (95-97%, d = 1.84 g/mL) were from Sigma-Aldrich. High-purity water (18.2 M •cm resistivity at 25 °C), produced by an Elix-Milli Q Academic system from Millipore (Vimodrone, MI, Italy), was used for standard and eluent preparation.
7
Silver Nanoparticle Dispersion Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Nearly monodisperse sodium citrate stabilized silver AgNPs dispersions of 20, 30, 40, 60, 70 and 100 nm at a nominal concentration of 20 mgL -1 were kindly donated by the Joint Research Centre, Institute for Health and Consumer Protection, Ispra (Italy). To avoid silver particle degradation or precipitation, dispersions were stored at 4 °C and protected from prolonged exposure to light.
Information regarding the sizes and the actual total silver concentrations, determined with complementary techniques by the JRC are reported in Tables 1 and2 respectively.
NaOH and sodium citrate (Carlo Erba Reagents -Italy) were used to prepare the mobile phase (eluent) for the SdFFF instrument.
All solutions were prepared using ultrapure deionised water (18 MΩ cm -1 ) obtained from a MilliQ system (Merck Millipore Milan, Italy).
Information regarding the sizes and the actual total silver concentrations, determined with complementary techniques by the JRC are reported in Tables 1 and2 respectively.
NaOH and sodium citrate (Carlo Erba Reagents -Italy) were used to prepare the mobile phase (eluent) for the SdFFF instrument.
All solutions were prepared using ultrapure deionised water (18 MΩ cm -1 ) obtained from a MilliQ system (Merck Millipore Milan, Italy).
8
Fungal Respiration Measurement Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The biological activity of each fungus was measured indirectly by the manometric method. The fungi were inoculated in 1 L septum sealed nozzle bottles filled with 100 mL of MEA medium, with the exception for E. halophilicum and A. penicillioides which MEA15% medium was used. All the bottles were closed with a pressure sensor mounted on top (OxiTop®-C system, WTW, Weilheim, Germany) and incubated for 25-35 d at 25 °C. The carbon dioxide evolved during aerobic respiration was quantitative adsorbed using droplets of NaOH (Carlo Erba Reagents, Milan, Italy) inside the sealed nozzle septum of the bottles. The pressure drop detected (every 100 min) in the bottle was proportional to the amount of oxygen used by fungi (Pereira et al., 2014; Schuchardt and Kruse, 2009; Willcock and Magan, 2001) .
9
Synthesis and Characterization of Functionalized SBA-15
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All reagents were of analytical grade. SBA-15, iron (III) nitrate nonahydrate (98%), (3aminopropyl) triethoxysilane (APTES, 99%), toluene (99.8%), ethanol and acetone (>99%), were purchased from Sigma Aldrich (Chemie, Steinheim, Germany). HCl (35% w/w, d= 1,187 g/ml) and NaOH (>98%) were from Carlo Erba (Milano, Italy). Glyphosate, NaCl (for ionic strength control) and H2SO4 (95-97%, d=1.84 g/ml) were from Sigma-Aldrich. High-purity water (18.2 MΩcm resistivity at 25 °C), produced by an Elix-Milli Q Academic system (Millipore, Vimodrone, MI, Italy) was used for standard and eluent preparation.
10
Organic Trace Analysis Solvents
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Solvents for organic trace analysis (cyclohexane, dichloromethane, n-hexane and toluene) were from J.T. Baker (Deventer, The Netherlands), ethanol was from Merck (Darmstadt, Germany) and nonane was purchased from Fluka Chemie (St. Gallen, Switherland). Silica and basic alumina for clean-up and fractionation were obtained from J.T. Baker and MP Biomedicals (Eschwege, Germany), respectively. Sulfuric acid (Merck) and sodium hydroxide (Carlo Erba, Milano, Italy) were also used to prepare modified silica.
Standard solutions of 13 C-labelled DL-PCBs for quantification (WP-LCS) and analytical recovery of the samples (WP-ISS), as well as calibration standards (WP-CS1 to WP-CS7), were from Wellington Laboratories Inc. (Guelph, Ontario, Canada).
Standard solutions of 13 C-labelled DL-PCBs for quantification (WP-LCS) and analytical recovery of the samples (WP-ISS), as well as calibration standards (WP-CS1 to WP-CS7), were from Wellington Laboratories Inc. (Guelph, Ontario, Canada).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!