Antibody binding competition assay was performed using Bio-Layer Interferometry (BLI) on Octet QK instrument (ForteBio). The first mAb (30 μg/mL) was immobilized onto the anti-human IgG Fc capture (AHC) (Fortebio, China). After washing with PBS, the biosensor tips were immersed into a well containing the wildtype SARS-CoV-2 RBD Protein (SinoBiological, Beijing, China) at a concentration of 51.5 μg/mL and loaded into a well containing the secondary mAb at a concentration of 30 μg/mL.
Anti human igg fc capture ahc
Manufactured by Molecular Devices
Sourced in China, United States
The Anti-human IgG Fc Capture (AHC) is a lab equipment product designed for the capture and purification of human immunoglobulin G (IgG) antibodies. It functions by selectively binding to the Fc region of human IgG. The AHC can be used in various immunological and biochemical applications that require the isolation and concentration of human IgG.
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Lab products found in correlation
3 protocols using anti human igg fc capture ahc
1
Antibody Binding Affinity by BLI
2
Kinetic Analysis of Influenza Antibodies
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CR9114 and S9-3-37 IgG at 5 μg/mL or MEDI8852, C05, and HIV PG9 Fab fragments at 20 μg/mL were loaded onto anti-human IgG Fc Capture (AHC) or anti-human Fab CH1 (FAB2G) biosensors (ForteBio, Fremont, CA, USA) in kinetics buffer (0.01% BSA + 0.002% Tween20 in 1× phosphate-buffered saline) and dipped into wells containing HAstem (135 nM or 270 nM), CLP-HAstem (250 nM or 500 nM total protein), or kinetics buffer alone using an Octet Red96 instrument (ForteBio). After loading, association was measured for 700 s, followed by dissociation for 750 s in kinetics buffer. A baseline containing kinetics buffer was subtracted from each data set, and curves were aligned on the y axis using the baseline step.
3
Stable mAb Expression in LP Cell Lines
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mAb payload vectors expressing the JUG444 human monoclonal antibody (Pfizer) were integrated into LP cell lines, selected for two weeks with puromycin (8 μg/ml or 20 μg/ml for mAb payload integrated into a single or multiple LPs, respectively) and maintained either as cell pools or as clonal populations following FACS. For mAb expression stability analysis, a 6-well master plate of cells was continuously propagated in cHAMS-F12 and used weekly for seeding cells for a mAb expression assay. 1.5 × 105 cells were seeded in 2–3 replicates in a 24-well plate with 0.5 ml cHAMS-F12 media and grown at 37°C. On day 4, conditioned media was collected and the amount of secreted JUG444 was measured in duplicates with an Octet RED96 system using anti-human IgG Fc Capture (AHC) or Protein A biosensors (ForteBio), with no difference in the measurements observed between the sensors. Purified JUG444 was used to generate a standard curve, from which mAb titers were derived.
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