Aiming to confirm the previous classification of the samples into the distinct HBV phases, serum samples were re-tested by commercial enzyme immunoassays (ELISA) for HBsAg (ETI-MAK-4, Diasorin, Italy), total anti-HBc (FTE AB COREKPLUS, Diasorin, Italy), anti-HBc IgM (TSI CORE IgMK Plus, Diasorin, Italy), anti-HBs (ETI-AB-AUK-3 Diasorin, Italy) and by electrochemiluminescence for HBeAg and anti-HBe serological markers (e411 Cobas, Roche Diagnostics, USA), according to the instructions of each manufacturer. In addition, antibodies to hepatitis C virus (Anti-HCV), hepatitis D virus (anti-HDV) and immunodeficiency virus (anti-HIV) were tested by commercially available immunoassays (Murex Anti-HCV, DiaSorin ETI-AB-DELTAK-2 and Murex HIV Ag/Ab Combination) to evaluate co-infections.
Anti hcv
Manufactured by DiaSorin
The Anti-HCV is a laboratory diagnostic product designed to detect the presence of antibodies to the hepatitis C virus (HCV) in human serum or plasma samples. It is used as a screening tool to identify individuals who have been exposed to the hepatitis C virus.
Automatically generated - may contain errors
2 protocols using anti hcv
1
Evaluating Hepatitis Virus Serological Markers
2
Serum Biomarkers for Liver Disease and Viral Hepatitis
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Serum levels of biochemical markers of liver function as alanine-aminotransferase (ALT), aspartate-aminotransferase (AST), gama-glutamyltransferase (GGT), direct and indirect bilirubin and alkaline phosphatase (AP) were determined using a photometric technique (ICMA) (Liason, Diasorin, Varceli, Italy).
Serum samples were used for detection of HBV, hepatitis C virus (HCV) and human immunodeficiency virus (HIV) serological markers with commercial tests. The detection of HBsAg (Biokit, Buenos Aires, Argentina), anti-HBc (Biokit, Buenos Aires, Argentina), anti-HBc IgM (Symbiosis Diagnóstica, Leme, Brazil), anti-HBs (Biokit, Buenos Aires, Argentina), anti-HIV (RPC Diagnostic Systems, Yablonevaya, Russia) and anti-HCV (Diasorin, São Paulo, Brazil) were performed using immunoenzymatic commercial methods, according to the instructions of each manufacturer. HBeAg (Roche Diagnostics, Rotkreuz ZG, Switzerland) and anti-HBe (Roche Diagnostics, Rotkreuz ZG, Switzerland) were performed in HBsAg positive samples.
Serum samples were used for detection of HBV, hepatitis C virus (HCV) and human immunodeficiency virus (HIV) serological markers with commercial tests. The detection of HBsAg (Biokit, Buenos Aires, Argentina), anti-HBc (Biokit, Buenos Aires, Argentina), anti-HBc IgM (Symbiosis Diagnóstica, Leme, Brazil), anti-HBs (Biokit, Buenos Aires, Argentina), anti-HIV (RPC Diagnostic Systems, Yablonevaya, Russia) and anti-HCV (Diasorin, São Paulo, Brazil) were performed using immunoenzymatic commercial methods, according to the instructions of each manufacturer. HBeAg (Roche Diagnostics, Rotkreuz ZG, Switzerland) and anti-HBe (Roche Diagnostics, Rotkreuz ZG, Switzerland) were performed in HBsAg positive samples.
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