For SEM sample preparation, drops of the EOP suspensions were adsorbed onto a glass coverslip and air-dried at 25 °C. The cover slips were then attached to aluminum stubs using carbon tape and sputter-coated with gold in a Balzers MED 010 unit (Oerlikon Balzers, Balzers, Liechtenstein). Spray-coated wood samples and a native wood sample were cut to a size of ca. 5 × 5 mm, attached to aluminum stubs, and sputter-coated before SEM analysis. SEM analysis was conducted using a JSM 6010LA electron microscope (JEOL Ltd., Tokyo, Japan).
Jsm 6010la electron microscope
Manufactured by JEOL
Sourced in Japan
The JSM 6010LA is a scanning electron microscope (SEM) manufactured by JEOL. It is designed to provide high-resolution imaging and analysis of a wide range of samples. The JSM 6010LA utilizes a thermionic electron gun to generate the electron beam and features a large specimen chamber to accommodate various sample types. The instrument is capable of producing detailed, high-quality images with its advanced imaging capabilities.
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9 protocols using jsm 6010la electron microscope
1
SEM Sample Preparation for Wood and EOP Analysis
2
Specimen Preparation for Electron Microscopy
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Specimens were processed for electron microscopy according to standardized procedures. Briefly, the samples were fixed in glutaraldehyde, rinsed in sodium cacodylate buffer, and secondarily fixed in osmium tetroxide before dehydrating in a graduated ethanol series. Following dehydration, the samples were mounted on a SEM stub and sputter coated for 30 s using a gold/palladium target in a Lecia (Buffalo Grove, IL) EM ACE 200 Vacuum Coater. Scanning electron micrographs were acquired using a JEOL (Peabody, MA) JSM-6010LA electron microscope operated in high-vacuum mode at 20kV. A minimum of three embryos were analyzed per genotype.
3
Scanning Electron Microscopy of Shoot Organogenesis
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To better understand when the shoot organogenesis occurs, early observations have been done by SEM analyses. Samples were fixed overnight at 4°C with 2.5% (v/v) glutaraldehyde + 2% (v/v) paraformaldehyde in 0.1 M cacodylate buffer, pH 7.2. After 3x20 min washings at 4°C in the same buffer, samples were post-fixed with 2% (v/v) osmium tetroxide in 0.1M cacodylate buffer, pH 7.2 for 2 h at 4°C. Specimens were washed in the same buffer (3 changes for 15 min each at 4°C), and then dehydrated in a graded ethanol series. Samples were dried by the critical point method using CO2 in a Balzers Union CPD 020. Then samples were attached to aluminum stubs using carbon tape and sputter-coated with gold in a Balzers MED 010 unit. The observations were made by a JEOL JSM 6010LA electron microscope.
4
Microscopic Analysis of Lipid Nanoparticles
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For preparation
of the samples for SEM, drops of the EO-LNP suspensions as well as
drops of redispersed freeze-dried EO-LNPs were adsorbed onto a glass
coverslip and air dried at 25 °C. The cover slips were in turn
attached to aluminum stubs using carbon tape and sputter-coated with
gold in a Balzers MED 010 unit (Oerlikon Balzers, Balzers, Liechtenstein).
SEM analysis was conducted with a JSM 6010LA electron microscope (JEOL
Ltd., Tokyo, Japan).
of the samples for SEM, drops of the EO-LNP suspensions as well as
drops of redispersed freeze-dried EO-LNPs were adsorbed onto a glass
coverslip and air dried at 25 °C. The cover slips were in turn
attached to aluminum stubs using carbon tape and sputter-coated with
gold in a Balzers MED 010 unit (Oerlikon Balzers, Balzers, Liechtenstein).
SEM analysis was conducted with a JSM 6010LA electron microscope (JEOL
Ltd., Tokyo, Japan).
5
Scanning Electron Microscopy of LNPs
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For scanning electron microscopy (SEM), the LNPs-coated samples were attached to aluminum stubs using carbon tape and sputter-coated with gold in a Balzers MED 010 unit. SEM analysis was conducted with a JSM 6010LA electron microscope (JEOL Limited, Tokyo, Japan). Size measurements of LNPs deposited on the wood surfaces were conducted on the SEM micrographs using the Adobe Photoshop CS4 Extended software package (Adobe Systems, San Jose, CA, USA). SEM experiments were carried out on samples before and after artificial weathering, according to the different steps.
6
Collagen Scaffold Preparation and SEM Imaging
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Collagen scaffolds inclusive of their cellular loads were cut into a number of small blocks with a sterile razor blade and fixed overnight at 4°C with 3% glutaraldehyde in 0.05 M phosphate buffer (PB) at pH 7.2. Following extensive rinsing with the same buffer at 4°C, blocks were soaked for 1 h in 0.5% tannic acid in PB at 4°C. Then they were rinsed four times in the same buffer for 15 min at 4°C, and postfixed with 1% osmium tetroxide in PB for 1 h at 4°C. Samples were washed in distilled water, dehydrated in a graded series of ethanol, and freeze-dried in a Balzers Union CPD 020 (Balzers, Liechtenstein) using the procedure of critical point drying. Samples were eventually sputter coated with gold in a Balzers MED 010 unit and observed in a JEOL JSM 6010LA electron microscope (Japan).
7
Drosophila Microscopic Preparation
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D. melanogaster flies were fixed and dehydrated as previously described [26 (link)]. Samples were dried by the critical point method using CO2 in a Balzers Union CPD 020 apparatus (Balzers Union Limited, Balzers, Liechtenstein). Then, the samples were attached to aluminum stubs using carbon tape and sputter-coated with gold in a Balzers Union MED 010 unit. The observations of eyes and wings were made by a JSM 6010LA electron microscope (Jeol, Tokyo, Japan).
8
Drosophila Head Preparation for SEM
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D. melanogaster heads were fixed and dehydrated as described for TEM. Samples were dried by the critical point method using CO2 in a Balzers Union CPD 020 apparatus (Balzers, Liechtenstein). Then samples were attached to aluminum stubs using a carbon tape and sputter-coated with gold in a Balzers Union MED 010 unit. The observations were made by a JSM 6010LA electron microscope (Jeol, Tokyo, Japan).
9
SEM Analysis of Catalysts
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For Scanning Electron Microscopy (SEM), catalysts (I –IV ) were fixed with 3% GA in 0.1 M cacodylate buffer (pH 7.2), washed, and post-fixed in OsO4 (1.0% in weight) in the same buffer at room temperature. The samples were dehydrated in ethanol series before the analysis, air dried, and sputter-coated with gold in a Balzers MED 010 unit (Balzers, Liechtenstein). The observation was made by a JEOL JSM 6010LA electron microscope (Tokyo, Japan).
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