Blood was collected from mice (50 μL in each staining tube). Each tube received 1 × Lysis Buffer (Biolegend, San Diego, CA, USA) followed by incubation at 37 °C for 10 to 15 min. The cells were centrifuged at 300 × g for 5 min. The supernatant was removed and the cells were stained with peridinin chlorophyll protein complex (PerCP) anti-mouse CD45 antibody, APC anti-mouse CD3 antibody, PE anti-mouse CD8b antibody, FITC anti-mouse CD4 Antibody, True-Nuclear™ One Step Staining Mouse Treg Flow™ Kit (FOXP3 Alexa Fluor® 488/CD25 PE/CD4 PerCP), and FITC anti-mouse IgG1 antibody, PE anti-mouse IgG1 antibody, and APC anti-mouse IgG1 antibody (all from Biolegend, San Diego, CA, USA) as an isotype control. Samples were run on a BD Canto II flow cytometer. The percentage of proliferating T cells was analyzed using FlowJo version 10.
True nuclear one step staining mouse treg flow kit foxp3 alexa fluor 488 cd25 pe cd4 percp
Manufactured by BioLegend
Sourced in United States
The True-Nuclear™ One Step Staining Mouse Treg Flow™ Kit (FOXP3 Alexa Fluor® 488/CD25 PE/CD4 PerCP) is a laboratory tool for the identification and analysis of mouse regulatory T cells (Tregs) using flow cytometry. The kit provides reagents for the simultaneous detection of FOXP3, CD25, and CD4 in a single-step staining procedure.
Automatically generated - may contain errors
Lab products found in correlation
Canto 2 flow cytometer, by BD (1 mentions)
Fitc anti mouse cd4 antibody, by BioLegend (1 mentions)
Apc anti mouse cd3 antibody, by BioLegend (1 mentions)
Lysis buffer, by BioLegend (1 mentions)
Pe anti mouse igg1 antibody, by BioLegend (1 mentions)
Mouse mdsc flow kit, by BioLegend (1 mentions)
Cd11b pe, by BioLegend (1 mentions)
Accuri c6, by BD (1 mentions)
2 protocols using true nuclear one step staining mouse treg flow kit foxp3 alexa fluor 488 cd25 pe cd4 percp
1
Comprehensive T Cell Analysis in Murine Blood
2
Quantifying Myeloid-Derived Suppressor Cells and Regulatory T Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For MDSCs assessment, PBMCs were obtained from the blood of mice as described above. Cells were labeled with a cocktail of CD11b‐PE (clone M1/70), Gr‐1‐APC (clone RB6/8C5), and Ly‐6 G‐FITC (clone 1A8) using the Mouse MDSC Flow kit (from Biolegend) following the manufacturer’s instructions.
For Tregs evaluation, PBMCs were obtained from the blood of mice as described above. Cells were labeled using a True Nuclear One Step Staining Mouse Treg Flow kit (FOXP3-AlexaFluor488, CD25-PE, CD4-PerCP; Biolegend) following the manufacturer’s instructions.
Cells were assessed in a BD Accuri C6 flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA), and data was analyzed using FlowJo software (LLC, Ashland, OR, USA).
For Tregs evaluation, PBMCs were obtained from the blood of mice as described above. Cells were labeled using a True Nuclear One Step Staining Mouse Treg Flow kit (FOXP3-AlexaFluor488, CD25-PE, CD4-PerCP; Biolegend) following the manufacturer’s instructions.
Cells were assessed in a BD Accuri C6 flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA), and data was analyzed using FlowJo software (LLC, Ashland, OR, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!