A total of 13 batches of FP were collected from Zhejiang (S1), Guangdong (S2 and S3), Guangxi (S4, S5 and S6), Hebei (S8), Hunan (S9, S10, S11 and S12) and Sichuan (S13) provinces of China. Besides, the control herbs (S7) were purchased from the National Institutes for Food and Drug Control (Beijing, China). Reference standards (purity >98%) including rutin, daidzin, daidzein, and quercetin were obtained from the National Institutes for Food and Drug Control (Beijing, China). Glycitin, genistin, tectoridin, glycitein, genistein, tectorigenin, and biochanin A with the purities over 98% were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Nicotiflorin and ononin with the purities over 98% were bought from Chengdu Alfa Biotechnology Co., Ltd. (Chengdu, China). Meanwhile, formononetin and puerarin were bought from Chengdu Must Bio-Technology Co., Ltd.(Chengdu, China). HPLC grade acetonitrile (ACN) and methanol (MeOH) were obtained from Fisher Scientific (Fair Lawn, NJ, USA). Formic acid of HPLC grade was provided by Kermel Chemical Reagent Co., Ltd. (Tianjin, China) and pure water was obtained from Wahaha (Hangzhou, China). All the other reagents were of analytical grade.
Glycitin
Manufactured by Yuanye Bio-Technology
Sourced in China
Glycitin is a laboratory equipment used for the analysis and detection of glycitins, a group of isoflavone derivatives found in various plant species. The core function of Glycitin is to provide accurate and reliable measurements of glycitin concentrations in samples.
Automatically generated - may contain errors
Lab products found in correlation
Genistin, by Yuanye Bio-Technology (6 mentions)
Glycitein, by Yuanye Bio-Technology (5 mentions)
Genistein, by Yuanye Bio-Technology (5 mentions)
Daidzin, by Yuanye Bio-Technology (5 mentions)
Daidzein, by Yuanye Bio-Technology (4 mentions)
Methanol meoh, by Thermo Fisher Scientific (1 mentions)
Biochanin a, by Yuanye Bio-Technology (1 mentions)
Hplc grade acetonitrile acn, by Thermo Fisher Scientific (1 mentions)
Tectorigenin, by Yuanye Bio-Technology (1 mentions)
Tectoridin, by Yuanye Bio-Technology (1 mentions)
Puerarin, by Chengdu Must Bio-Technology (1 mentions)
Formononetin, by Chengdu Must Bio-Technology (1 mentions)
Propionic acid, by Merck Group (1 mentions)
3 5 dinitrosalicylic acid, by Sinopharm (1 mentions)
Butyric acid, by Merck Group (1 mentions)
Isovaleric acid, by Merck Group (1 mentions)
Caproic acid, by Merck Group (1 mentions)
Valeric acid, by Merck Group (1 mentions)
Isobutyric acid, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
6 protocols using glycitin
1
Comprehensive Analysis of Fructus Puerariae
2
Quantification of Soy Isoflavones and SCFAs
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Sodium chloride (NaCl) and foline-phenol (C6H6O) were purchased from Tianjin Fengchuan Chemical Reagent Co., Ltd. Potassium dihydrogen phosphate (KH2PO4) and 3,5-dinitrosalicylic acid (DNS) were purchased from Sinopharm Chemical Reagent Co., Ltd. Disodium hydrogen phosphate (Na2HPO4) was purchased from Tianjin Hengxing Chemical Reagent Manufacturing Co., Ltd. SIs (H05M9R54983), composed of daidzin (57.98%), glycitin (23.09%), genistin (6.92%), daidzein (2.12%), glycitein (0.74%), and genistein (0.4%), were purchased from Shanghai Yuanye Biotechnology Co., Ltd.
Reference standards, including acetic acid (≥ 99.5%), propionic acid (≥ 99.0%), isobutyric acid (≥ 99.0%), butyric acid (≥ 99.0%), isovaleric acid (≥ 99.0%), valeric acid (≥ 98%), caproic acid (≥ 99.5%), and isocaproic acid (≥ 98%), were purchased from Sigma-Aldrich (Merck, Darmstadt, Germany).
Reference standards, including acetic acid (≥ 99.5%), propionic acid (≥ 99.0%), isobutyric acid (≥ 99.0%), butyric acid (≥ 99.0%), isovaleric acid (≥ 99.0%), valeric acid (≥ 98%), caproic acid (≥ 99.5%), and isocaproic acid (≥ 98%), were purchased from Sigma-Aldrich (Merck, Darmstadt, Germany).
3
Extraction and Purification of Soybean Compounds
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The stachyose (pure>80%) was extracted from soybean residue [22 (link)]. Soybean isoflavones (pure>95%) includes 50% of daidzin, 6% of daidzein, 26% of glycitin, 3% of glycitein, 10% of genistin and 2% of genistein for animal experiments and the analytical standards of daidzein, glycitein and genistein (pure>98%) were bought from Shanghai Yuanye Biotechnology co., Ltd. (Shanghai, China). Assay kits of total cholesterol (TC), total triglyceride (TG), high-densitylipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C), glutamic-oxal(o)acetic transaminase (GOT) and glutamicpyruvic transaminase (GPT) were purchased from HuiliBiotechnology Co. Ltd. (Changchun, China). Additionally, enzyme linked immunosorbent assay ELISA kits of interleukin-1 (IL-1), tumor necrosis factor (TNF-α), nuclear factor kappa-B (NF-κB), acetyl CoA carboxylase (ACC), fatty acid synthetase (FAS), glucose, insulin, leptin and lipopolysaccharide (LPS) were purchased from Jiancheng Bioengineering Institute (Nanjing, China). All other reagents and chemicals were analytical grade or higher.
4
Soy Isoflavones as Alzheimer's Therapy
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Soy isoflavones (SI) (purify > 80% by HPLC) was purchased from Yuanye Biological Technology Co., Ltd. (Shanghai, China) and its chemical composition mainly included genistin 53.82%, daidzin 15.49% and glycitin 12.63%. Scopolamine (SCOP) was purchased from Sigma Chemical Co., Ltd. (St. Louis, MO, USA) and donepezil hydrochloride (Aricept) (DNP) was obtained from Eisai (Ibaraki, Japan). Carboxy methyl cellulose sodium (CMC-Na) was purchased from Shenggong Biological Engineering Co., Ltd. (Shanghai, China). Acetylcholinesterase (AChE), choline acetyltransferase (ChAT), malondialdehyde (MDA) and glutathione (GSH) commercial kits were obtained from Jiancheng Biological Technology Co., Ltd. (Nanjing, Jiangsu, China).
5
Soybean Fermentation with R. oligosporus
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The high purity (≥97%) standards, including ascorbic acid, vanillic acid, syringic acid, epicatechin, daidzin, glycitin, ferulic acid, genistin, daidzein, glycitein, quercetin, and genistein were purchased from Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Other reagents of analytical grade. Soybeans (Liaodou, yellow seed coat, mainly planted in northern China, weigh 21.6 g of one hundred seeds) were obtained from a local market. R. oligosporus RT-3 isolated, screened and identified from Tempeh in our laboratory was used for soybean fermentation (11 (link), 12 ). The strain was cultivated on potato dextrose agar at 30°C for 3 days. Then, spores produced were scraped in water (1 × 107 spores/mL).
6
Fermentation of Soybean Isoflavones
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Daidzein, glycitein, genistein, daidzin, glycitin, and genistin were purchased from Yuanye Biotechnology Co., Ltd. (Shanghai, China) with a purity ≥98%. Methanol (HPLC grade) was purchased from Thermo Fisher Scientific (Shanghai, China). All other chemicals and reagents were analytical grade, and reverse-osmosis Milli-Q water was used.
The fresh soybeans were purchased from the local supermarket. Bacillus subtilis natto ATCC 65301 used for fermentation was purchased from American Type Culture Collection (ATCC) and maintained in 20% glycerol at −80 °C. Its cultural conditions and activation methods are applied according to the report of Li [21 (link)].
The fresh soybeans were purchased from the local supermarket. Bacillus subtilis natto ATCC 65301 used for fermentation was purchased from American Type Culture Collection (ATCC) and maintained in 20% glycerol at −80 °C. Its cultural conditions and activation methods are applied according to the report of Li [21 (link)].
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