Protein oxidation detection kit

Manufactured by Merck Group

Sourced in United States

The Protein Oxidation Detection Kit is a laboratory tool designed to detect and quantify the level of oxidative modifications in proteins. It provides a reliable and efficient method for researchers to assess the extent of protein oxidation within a sample.

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Lab products found in correlation

Abioruptor, by Diagenode (1 mentions)

Spelling variants of this product

OxyBlot Protein Oxidation Detection Kit (278 citations) Oxyblot Protein Oxidation Detection Analysis Kit (2 citations)

3 protocols using protein oxidation detection kit

Protein Carbonyl Detection in Muscle

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Protein Oxidation Detection kit was used as recommended by the manufacturer (Millipore, Billerica, MA, USA). Briefly, muscle extracts were derivitized with 2,4‐dinitrophenylhydrazine and probed for protein carbonyls using an antibody for DNP.

Kruse S.E., Karunadharma P.P., Basisty N., Johnson R., Beyer R.P., MacCoss M.J., Rabinovitch P.S, & Marcinek D.J. (2015). Age modifies respiratory complex I and protein homeostasis in a muscle type‐specific manner. Aging Cell, 15(1), 89-99.

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Quantitative Carbonyl Protein Oxidation Assay

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Carbonyl groups were detected using the Protein Oxidation Detection Kit
(Millipore, S7150). Briefly, pellets of day 1 adult worms (1-2 60 mm plates per
sample) were collected and flash frozen in lysis Buffer (150 mM KCl, 1mM EDTA,
0.25% SDS, 1% NP-40, 50 mM Tris/HCL pH 7.4), with DTT added to a
final concentration of 50mM to prevent oxidation occurring after cell lysis.
Samples were freeze-thawed on ice, and sonication was performed using a
Bioruptor (Diagenode). Protein concentration of diluted samples (5mM DTT) was
determined using the reducing agent compatible BCA kit (Pierce/Thermoscientfic
23250). 5 μg total protein was put into each DNPH and control reaction,
and separated on a 10% gel, transferred, and developed according to
standard methods. Quantification was performed using ImageJ by collecting total
pixel intensity of equally thresholded ROI, with the negative control background
subtracted from each sample. Intensity was normalized to WT, and 3 biological
replicates were averaged.

Dues D.J., Schaar C.E., Johnson B.K., Bowman M.J., Winn M.E., Senchuk M.M, & Van Raamsdonk J.M. (2017). Uncoupling of Oxidative Stress Resistance and Lifespan in Long-lived isp-1 Mitochondrial Mutants in Caenorhabditis elegans. Free radical biology & medicine, 108, 362-373.

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Protein Carbonylation Detection

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The detection of protein carbonylation was performed using a Protein Oxidation Detection Kit (Millipore, Billerica, MA), according to the manufacturer’s instructions.

Namba F., Go H., Murphy J.A., La P., Yang G., Sengupta S., Fernando A.P., Yohannes M., Biswas C., Wehrli S.L, & Dennery P.A. (2014). Expression Level and Subcellular Localization of Heme Oxygenase-1 Modulates Its Cytoprotective Properties in Response to Lung Injury: A Mouse Model. PLoS ONE, 9(3), e90936.

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