The following antibodies were purchased from BD Biosciences (San Jose, CA, USA): fluorescein isothiocyanate (FITC)-conjugated anti-CD14 (MφP9), anti-CD4 (SK3), and anti-CD19 (SJ25C1); phycoerythrin (PE)-conjugated anti-CD3 (SP34-2); peridinin chlorophyll protein complex (PerCP)-conjugated anti-CD3 (SK7); Alexa Fluor 647-conjugated anti-CD8 (RPA-T8) mouse anti-human monoclonal antibodies; and PE-conjugated anti-rabbit secondary antibody. Anti-phosphorylated (anti-p)-JAK1 (PhosphoDetect Tyr1022/1023) rabbit anti-human antibody was purchased from EMD Millipore (Darmstadt, Germany). The following antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA): anti-pJAK2 (Tyr1007/Tyr1008), anti-pJAK3 (Tyr980), anti-pTyk2 (Tyr1054) rabbit anti-human antibodies, and PerCP-conjugated anti-rabbit secondary antibody. The amounts of the antibodies were optimized, and their compatibility was assured in preliminary examinations.
Anti cd19 sj25c1
Manufactured by BD
Sourced in United States
Anti-CD19 (SJ25C1) is a mouse monoclonal antibody that recognizes the CD19 antigen. CD19 is a cell surface protein expressed on B cells and B-cell lineage malignancies. This antibody can be used for the identification and enumeration of B cells in flow cytometric analysis.
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Lab products found in correlation
Anti cd3 ucht1, by BioLegend (1 mentions)
Anti cd45 hi30, by BioLegend (1 mentions)
Anti cd47 cc2c6, by BioLegend (1 mentions)
Cc2c6, by BioLegend (1 mentions)
Anti cd14 mφ p9, by BD (1 mentions)
Anti cd56 ncam16.2, by BD (1 mentions)
Qifikit, by Agilent Technologies (1 mentions)
Facscanto 2 flow cytometer, by BD (1 mentions)
2 protocols using anti cd19 sj25c1
1
Flow Cytometry Antibody Panel
2
Quantifying Immune Cell Surface Markers
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For the FACS staining, 1,00,000 cells were seeded and stained with the indicated antibodies in FACS buffer (PBS, 0.01% bovine serum albumin, 0.01% sodium-azide) for 45 min on ice. Expression level of SIRPα (15–414, Biolegend) on various immune cell subsets within whole peripheral blood was determined using the following antibodies to determine different immune cell subsets: Anti-CD45 (HI30, Biolegend), anti-CD19 (SJ25C1, BD Biosciences), anti-CD3 (UCHT1, Biolegend), anti-CD14 (MΦP9, BD Biosciences), anti-CD56 (NCAM16.2, BD Biosciences) and anti-CD66b (G10F5, BD Biosciences).
Surface expression of GD2 on neuroblastoma cell lines was determined using an anti-GD2 antibody (14G2a, Biolegend) while CD47 expression was measured using anti-CD47 (CC2C6, Biolegend) antibody.
To quantify the number of CD47 molecules per cell, we performed a QIFIKIT (Agilent/Dako) analysis according to the manufacturer’s instructions. Cells were stained with a saturating concentration of 10 µg/mL unconjugated mouse IgG monoclonal antibody directed against CD47 (CC2C6, Biolegend). Measurements were performed using a BD FACS Canto II flow cytometer.
Surface expression of GD2 on neuroblastoma cell lines was determined using an anti-GD2 antibody (14G2a, Biolegend) while CD47 expression was measured using anti-CD47 (CC2C6, Biolegend) antibody.
To quantify the number of CD47 molecules per cell, we performed a QIFIKIT (Agilent/Dako) analysis according to the manufacturer’s instructions. Cells were stained with a saturating concentration of 10 µg/mL unconjugated mouse IgG monoclonal antibody directed against CD47 (CC2C6, Biolegend). Measurements were performed using a BD FACS Canto II flow cytometer.
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