Bioplex htf

Manufactured by Bio-Rad

Sourced in United States

The Bioplex HTF is a high-throughput flow cytometry system designed for multiplexed analysis of biological samples. It enables the simultaneous measurement of multiple analytes in a single sample.

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Lab products found in correlation

Luminex 200 system, by Bio-Rad (4 mentions) Bio plex manager version 5, by Bio-Rad (3 mentions) Bio plex pro mouse cytokine 7 plex array kit, by Bio-Rad (2 mentions) Bradford assay kit, by Bio-Rad (1 mentions) Bio plex manager software, by Bio-Rad (1 mentions) Bio plex manager version 6, by Bio-Rad (1 mentions) Bio plex pro mouse cytokine 8 plex assay kit, by Bio-Rad (1 mentions)

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BioPlex HTF system (3 citations)

6 protocols using bioplex htf

Multiplex Cytokine Quantification

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Plasma and amniotic fluid cytokine levels (n = 5–6/group) were quantified using Bio-Plex Pro Mouse Cytokine 7-Plex Array kit (Bio-Rad). Multiplex assay was performed on Luminex 200 system and Bio-Plex HTF (Bio-Rad) in accordance with the manufacturer's instructions. Standards and each sample were analysed in duplicate. Data analysis was performed using Bio-Plex Manager, version 5.0 (Bio-Rad) and presented as concentrations (pg/ml).

Shynlova O., Dorogin A., Li Y, & Lye S. (2014). Inhibition of infection-mediated preterm birth by administration of broad spectrum chemokine inhibitor in mice. Journal of Cellular and Molecular Medicine, 18(9), 1816-1829.

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Multiplex Cytokine Assay Protocol

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The protein extraction procedure is the same as the one described above. Take 50 ul of each sample for multiplex assay with a Bio-Plex Pro Mouse Cytokine 7-Plex Array kit (Bio-Rad). Proteins were incubated with antibodies conjugated to Magplex microspheres under shaking for 2 h at room temperature. Then they were incubated with antibodies for 1 h and streptavidin phycoerythrin for 30 min at room temperature. Finally, the protein concentration was detected by Luminex 200 system and Bioplex HTF (Bio-Rad) and calculated according to standard sample protein concentration.

Chen L., Wang L., Luo Y., Huang Q., Ji K., Bao J, & Liu H. (2021). Integrated Proteotranscriptomics of Human Myometrium in Labor Landscape Reveals the Increased Molecular Associated With Inflammation Under Hypoxia Stress. Frontiers in Immunology, 12, 722816.

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Placental Cytokine and VEGF Profiling

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Placentas were collected as described above. Total lysates were prepared by homogenizing 60 mg of placental tissue in 800 μl of RIPA lysis buffer. Placental IL-1, IL-6, TNF-α and vascular endothelial growth factor (VEGF) levels in the different groups were detected using Luminex 200 system and Bioplex HTF (Bio-Rad, CA, U.S.A.) in accordance with the manufacturer’s instructions. Standards and each sample were analyzed in duplicate. Data analysis was performed using Bio-Plex Manager, version 5.0 (Bio-Rad) and presented as concentrations (pg/ml/mg).

Bao J., Zou Y., Liu Y., Yuan L., Garfield R.E, & Liu H. (2019). Nicotine protects fetus against LPS-induced fetal growth restriction through ameliorating placental inflammation and vascular development in late pregnancy in rats*. Bioscience Reports, 39(7), BSR20190386.

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Quantitative TGFβ Measurement in Deciduae

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Bio-Plex Pro™ TGFb assay was conducted according to manufacturer's instructions (Bio-Rad Laboratories Inc). Deciduae were homogenized in bicine buffer and total protein was measured by Bradford assay kit (Bio-Rad Laboratories Inc). HCl activated samples were loaded in a 96-well plate containing magnetic beads conjugated with antibodies to TGFb1/2/3. After incubation, the results were analyzed by a Luminex 200 cytometer and Bioplex HTF (Bio-Rad Laboratories Inc). Data analysis was performed with Bio-Plex Manager software (v5.0; Bio-Rad Laboratories Inc).

Zhang J., Dunk C.E., Shynlova O., Caniggia I, & Lye S.J. (2018). TGFb1 suppresses the activation of distinct dNK subpopulations in preeclampsia. EBioMedicine, 39, 531-539.

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Multiplex Cytokine Profiling in Inflammation

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To evaluate the effect of PMG on proinflammatory cytokines and chemokines, the levels of the proinflammatory cytokines IL1β, IL6, IL12, tumor necrosis factor (TNF)α, interferon (IFN)γ, and CSF2/GM-CSF, anti-inflammatory cytokine IL10, and 3 major chemokines CXCL1/KC, CCL2/MCP1, and CCL4/Mib1b were measured by the Luminex multiplex assay in the plasma and amniotic fluid using the Bio-Plex Pro Mouse Cytokine 10-Plex Array kit (Bio-Rad, Hercules, CA) (Supplemental Table 2). The magnetic bead kit was run on the Luminex 200 system and Bioplex HTF (Bio-Rad) in accordance with the manufacturer`s instructions. The standards and each of the samples were analyzed in duplicate. Data analysis was performed using the Bio-Plex Manager version 6.0 (Bio-Rad) and presented as concentrations (pg/mL). The Bio-Plex “Assay Working Range” and the sensitivity of each cytokine assay (www.biorad.com) is shown in Supplemental Table 2.

Shynlova O., Nadeem L., Dorogin A., Mesiano S, & Lye S.J. (2021). The selective progesterone receptor modulator-promegestone-delays term parturition and prevents systemic inflammation-mediated preterm birth in mice. American journal of obstetrics and gynecology, 226(2), 249.e1-249.e21.

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Cytokine Profiling of rL7/L12-Omp25 Vaccine

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The profile of cytokines induced by rL7/L12-Omp25 was determined using the culture supernatant of re-stimulated splenocytes of rL7/L12-Omp25, rL7/L12-Omp25 + rIFN-γ, B. abortus S19, and sham-immunized mice collected from the above experiment. Bio-Plex Pro Mouse Cytokine 8-plex assay kit (Catalog no. M60000007A, Bio-Rad, California) was used for quantification of cytokine level in the induced culture supernatant. The cytokine targets of detection were IFN-γ, TNF-α, IL-4, IL-5, IL-10, IL-2, IL-12(p70), and GM-CSF.
The assay was performed in multiplex format along with appropriate standards as per the instructions provided in the assay kit using Bio-Plex HTF (Bio-Rad, California). Analysis of the obtained data was performed using Bio-Plex Manager, version 5.0 (Bio-Rad, California). Sample showing out of range (OOR) concentration was considered as the highest extrapolated value for a conservative estimate and statistical analysis. Standard curve was generated for standards to use as reference for determination of concentration of each cytokine in analyzed samples. The experiment was performed thrice with induced culture supernatant from rL7/L12-Omp25, rL7/L12-Omp25 + rIFN-γ, B. abortus S19, and sham-immunized mice.

Paul S., Peddayelachagiri B.V., Nagaraj S., Konduru B, & Batra H.V. (2018). Protective and therapeutic efficacy study of divalent fusion protein rL7/L12-Omp25 against B. abortus 544 in presence of IFNγ. Applied microbiology and biotechnology, 102(20).

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