H3k27ac

Manufactured by Vector Laboratories

H3K27ac is a histone modification-specific antibody that recognizes acetylation of lysine 27 on histone H3. This antibody is a useful tool for studying epigenetic regulation and gene expression.

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Lab products found in correlation

2 protocols using h3k27ac

Characterization of Protein Expression

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The following antibodies were used in this study: R-IgG (SC-2027), m-IgG (SC-2025), actin (SC-47778), H3K4me3 (Cell Signaling, 9751; active motif 39159), H3K27me3 (Cell Signaling, 9733), H3K27ac (ab4729), H3K9ac (ab176916), rabbit polyclonal Ki67 (Vector Labs), hnRNPK (SC-28380), TDP43 (ab133547), SAFB1 (A300-811A), DDX3 (SC-365768), SC35 (ab11826), U2AF65 (SC-53942), DDX17 (SC-130650), SFRS10 (Bethyl, A305-011A), hnRNPA1 (SC-32301), hnRNPC (SC-32308), hnRNPG (Cell Signaling D7C2V), and DDX21 (SC-376953).
For the immunohistochemistry (IHC) studies, primary antibody was used at a 1:200 dilution and secondary at 1:1,000. For western blots, a 1:1,000 dilution was used for the primary and 1:10,000 for the secondary.

Puvvula P.K., Buczkowski S, & Moon A.M. (2021). hnRNPK-derived cell-penetratingpeptide inhibits cancer cell survival. Molecular Therapy Oncolytics, 23, 342-354.

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Comprehensive Antibody Panel for Epigenetics

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R-IgG (SC-2027), m-IgG (SC-2025), Actin (SC-47778), H3K9me3 (Cell Signaling, 9754), H3K4me3 (Cell Signaling, 9751; active motif 39159), H3K27me3 (Cell Signaling, 9733), H3K9ac (Cell Signaling, 9649), H3K36me (Cell Signaling, 4909), H3K27ac (ab4729), H3K9ac (ab176916), rabbit polyclonal Ki67 (Vectorlabs), MLL1 (Active motif, 61296), Lamin A/C (E-1), hnRNPC1/C2 (Santa Cruz, SC-32308), SAFA (Santa Cruz, SC-32315), U2AF65 (Santa Cruz, SC-53942), DDX3 (Santa Cruz, SC-365768), hnRNPA1 (Santa Cruz, SC-32301), hnRNPD (abcam, ab61193), DDX21 (Santa Cruz, SC-376953), DNA Damage antibody sample kit (Cell Signaling, 9947), Apoptosis Antibody sampler Kit (Cell signaling, 9915).

Puvvula P.K, & Moon A.M. (2021). Novel Cell-Penetrating Peptides Derived From Scaffold-Attachment- Factor A Inhibits Cancer Cell Proliferation and Survival. Frontiers in Oncology, 11, 621825.

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