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The HeLa Kyoto is a cell line derived from human cervical cancer cells. It is a widely used laboratory tool for various research purposes.

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Lab products found in correlation

Hek293, by American Type Culture Collection (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Hct116 cell, by American Type Culture Collection (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by American Type Culture Collection (2 mentions) Mccoy s 5a, by American Type Culture Collection (2 mentions) Silentfect, by Bio-Rad (2 mentions) Htert rpe1 cells, by American Type Culture Collection (2 mentions) Mda mb 231, by American Type Culture Collection (1 mentions) Dmem glutamax supplement, by Thermo Fisher Scientific (1 mentions) Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions) U2932, by American Type Culture Collection (1 mentions) Nunclon delta airvent, by Thermo Fisher Scientific (1 mentions)

3 protocols using hela kyoto

1

Cell Culture Conditions and Preparation

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Cells were grown at 37 °C and 5% ambient CO2. HeLa Kyoto (ATCC), MDA-MB-231 (ATCC), and A549 cells (ATCC) were grown to ~80% confluence in 140 × 20 mm dishes (NunclonTM Delta Airvent, Thermo Fisher) with Dulbecco’s Modified Eagle’s Medium, high glucose, GlutaMAXTM supplement (DMEM, Thermo Scientific), 10% fetal bovine serum (FBS), and 1% penicillin-streptomycin. H-358 (ATCC CRL-5807) and U-2932 (ATCC) cell lines were cultured in RPMI 1640 with GlutaMAXTM, 1% penicillin-streptomycin, and 10% FBS. Prior to HATRIC-LRC experiments with folate, HeLa cells were starved for 48 h in folate-free RPMI (Gibco).
Sobotzki N., Schafroth M.A., Rudnicka A., Koetemann A., Marty F., Goetze S., Yamauchi Y., Carreira E.M, & Wollscheid B. (2018). HATRIC-based identification of receptors for orphan ligands. Nature Communications, 9, 1519.
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2

Cell Culture Conditions and Transfection Protocols

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Cells were grown at 37 C and 5% CO2. HeLa Kyoto (ATCC), SW480 (a gift from Ryoma Ohi, Vanderbilt), and HEK293 (ATCC) cells were maintained in DMEM (Invitrogen) supplemented with 10% Fetal Bovine Serum (Life technologies); hTERT-RPE1 cells (ATCC) in Ham’s F12/DMEM (Mediatech) supplemented with 10% Fetal Bovine Serum and HCT116 cells (ATCC) were cultured in McCoy’s 5A (ATCC) supplemented with 10% Fetal Bovine Serum (Life technologies). For expression of recombinant proteins, HeLa cells were transfected with Lipofectamine 2000 (Life Technologies) 24 h prior to assay. For RNAi, two 48 h transfections were performed with 20nM siRNA for Stealth siRNAs or 5nM for Silencer Select siRNAs using Lipofectamine 2000 according to the manufacturer’s protocol. For RPE1 RNAi, 5nM of siRNA was transfected with Silentfect (BioRad) following manufacturer protocols. IFNγ was from R&D Serotec, JAKi from Calbiochem and Flag-TRAIL from Axxora. His-TRAIL was purified according to standard procedures42 (link).
Todorova T., Bock F.J, & Chang P. (2014). PARP13 regulates cellular mRNA post-transcriptionally and functions as a pro-apoptotic factor by destabilizing TRAILR4 transcript. Nature communications, 5, 5362.
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3

Cell Culture Conditions and Transfection Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were grown at 37 C and 5% CO2. HeLa Kyoto (ATCC), SW480 (a gift from Ryoma Ohi, Vanderbilt), and HEK293 (ATCC) cells were maintained in DMEM (Invitrogen) supplemented with 10% Fetal Bovine Serum (Life technologies); hTERT-RPE1 cells (ATCC) in Ham’s F12/DMEM (Mediatech) supplemented with 10% Fetal Bovine Serum and HCT116 cells (ATCC) were cultured in McCoy’s 5A (ATCC) supplemented with 10% Fetal Bovine Serum (Life technologies). For expression of recombinant proteins, HeLa cells were transfected with Lipofectamine 2000 (Life Technologies) 24 h prior to assay. For RNAi, two 48 h transfections were performed with 20nM siRNA for Stealth siRNAs or 5nM for Silencer Select siRNAs using Lipofectamine 2000 according to the manufacturer’s protocol. For RPE1 RNAi, 5nM of siRNA was transfected with Silentfect (BioRad) following manufacturer protocols. IFNγ was from R&D Serotec, JAKi from Calbiochem and Flag-TRAIL from Axxora. His-TRAIL was purified according to standard procedures42 (link).
Todorova T., Bock F.J, & Chang P. (2014). PARP13 regulates cellular mRNA post-transcriptionally and functions as a pro-apoptotic factor by destabilizing TRAILR4 transcript. Nature communications, 5, 5362.
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