WI2 anti-idiotype antibody specific for hMN14, the antibody portion of the anti-CEA CAR, was a gift of Hans Hansen (Immunomedics, Morris Plain, NJ). Mouse anti-pc, anti-V5, and anti-myc epitope antibodies were purchased from Invitrogen (Grand Island, NY). Phycoerythrin (PE)-conjugated anti-human Vb5.1 antibody (clone (IMMUl57) was purchased from Immunotech (Beckman Coulter, Pasedena, CA). JCV-specific p36 peptide (VP1p36-44: SITEVECFL)22 (link) (Invitrogen) was prepared as PE-conjugated p36/HLA-A*0201 tetramer by the NIH Tetramer Core Facility (Emory University, Atlanta, GA).
Retroviral packaging cell lines 293-gag-pol (gp) and PG 13 were obtained from American Type Culture Collection (ATCC, Manassas, VA). 293T p36 positive control cells were generated by transduction with tctv {p36VaCaz}{p36VbCbz} constructs and sorted to >95% tetramer and Vb5.1 expression using FACSVantage SE (Becton Dickinson, Franklin Lakes, NJ).
Retroviral packaging cell lines 293-gag-pol (gp) and PG 13 were obtained from American Type Culture Collection (ATCC, Manassas, VA). 293T p36 positive control cells were generated by transduction with tctv {p36VaCaz}{p36VbCbz} constructs and sorted to >95% tetramer and Vb5.1 expression using FACSVantage SE (Becton Dickinson, Franklin Lakes, NJ).