Coulter hmx al hematology analyzer

253 individual study participants consisting of 172 smokers and 81 nonsmokers enrolled between 1993 and 1995 as healthy volunteers from the general public in Durham and Chapel Hill, North Carolina. These subjects were part of a community-based sample comprised of 294 healthy unrelated blacks and whites; collection and processing have been described in several studies [3 ,4 (link),52 (link)]. An independent group of black and white females (20 smokers and 14 nonsmokers) was recruited at the NIEHS Clinical Research Unit (protocol 10-E-0063) between March 2013 and January 2015 from the Raleigh, Durham and Chapel Hill, NC area. All nonsmokers were self-reported as not having smoked >100 cigarettes in their lifetime. Smokers reported their average daily cigarette consumption for the past 3 months. Age and smoking history of all subjects are given in Tables 1 and 2 as average and range. Peripheral blood monocytes, lymphocytes, eosinophils, basophils and neutrophils were counted by an automated cell counter, Coulter HmX AL Hematology Analyzer (Beckman Coulter, UK) by the Hematological Laboratory at NIEHS. Serum nicotine/cotinine levels were measured by HPLC-MS (Quest, Inc). More details for all methods are provided in the Supporting files.