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Enzchek direct phospolipase c assay kit

Manufactured by Thermo Fisher Scientific
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The EnzChek® Direct Phospolipase C Assay Kit is a laboratory instrument used to measure phospholipase C activity. It provides a direct, continuous fluorometric method for quantifying phospholipase C activity.

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Lab products found in correlation

Amplex red phospholipase d assay kit, by Thermo Fisher Scientific (4 mentions) Bca reagent, by Bio-Rad (2 mentions) Nitrocellulose membrane, by Bio-Rad (2 mentions) Protease inhibitor, by Merck Group (1 mentions) Precision plus protein all blue standard, by Bio-Rad (1 mentions) Magellan software, by Tecan (1 mentions)

4 protocols using enzchek direct phospolipase c assay kit

1

Phospholipase Activity Assays

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BCA reagents, and nitrocellulose membranes were purchased from Bio-Rad (Hercules, CA, USA). All chemicals, protease, protease inhibitors and antibodies were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise noted. EnzChek® Direct Phospolipase C Assay Kit and Amplex® Red Phospholipase D Assay Kit were purchased from Invitrogen/Life Technologies (Carlsbad, CA, USA).
REN X., KEENEY J.T., MIRIYALA S., NOEL T., POWELL D.K., CHAISWING L., BONDADA S., ST. CLAIR D.K, & BUTTERFIELD D.A. (2018). The Triangle of Death of Neurons: Oxidative Damage, Mitochondrial Dysfunction, and Loss of Choline-Containing Biomolecules in Brains of Mice Treated with Doxorubicin. Advanced Insights into Mechanisms of Chemotherapy Induced Cognitive Impairment (“Chemobrain”) Involving TNF-α. Free radical biology & medicine, 134, 1-8.
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2

Phospholipase Activity Quantification

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Phosphatidylcholine-specific phospholipase C (PC-PLC) and phospholipase D (PLD) activity assays were performed using manufacturers’ instructions provided with the EnzChek® Direct Phospolipase C Assay Kit and Amplex® Red Phospholipase D Assay Kit by Invitrogen/Life Technologies (Carlsbad, CA). A SPECTRAFLUOR PLUS instrument was used to determine the fluorescence, quantified by Magellan™ software. The kinetic peaks of fluorescence of positive control were recorded over a period of 24h. The samples’ fluorescence were acquired at incubation time at the peak.
REN X., KEENEY J.T., MIRIYALA S., NOEL T., POWELL D.K., CHAISWING L., BONDADA S., ST. CLAIR D.K, & BUTTERFIELD D.A. (2018). The Triangle of Death of Neurons: Oxidative Damage, Mitochondrial Dysfunction, and Loss of Choline-Containing Biomolecules in Brains of Mice Treated with Doxorubicin. Advanced Insights into Mechanisms of Chemotherapy Induced Cognitive Impairment (“Chemobrain”) Involving TNF-α. Free radical biology & medicine, 134, 1-8.
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3

Quantifying Phospholipase Activity

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Precision Plus Protein™ All Blue Standards, BCA reagents, and nitrocellulose membranes were purchased from Bio-RAD (Hercules, CA, USA). EnzChek® Direct Phospolipase C Assay Kit and Amplex® Red Phospholipase D Assay Kit were purchased from Invitrogen/Life Technologies (Carlsbad, CA). Chemicals, proteases, protease inhibitors, and antibodies used in this study were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise noted.
Keeney J.T., Ren X., Warrier G., Noel T., Powell D.K., Brelsfoard J.M., Sultana R., Saatman K.E., Clair D.K, & Butterfield D.A. (2018). Doxorubicin-induced elevated oxidative stress and neurochemical alterations in brain and cognitive decline: protection by MESNA and insights into mechanisms of chemotherapy-induced cognitive impairment (“chemobrain”). Oncotarget, 9(54), 30324-30339.
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4

Phospholipase Activity Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Phosphatidylcholine-specific phospholipase C (PC-PLC) and phospholipase D (PLD) activity assays were performed using manufacturers’ instructions provided with the EnzChek® Direct Phospolipase C Assay Kit and Amplex® Red Phospholipase D Assay Kit by Invitrogen/Life Technologies (Carlsbad, CA), respectively. Fluorescence intensity for each assay was measured using a SPECTRAFluor Plus instrument and quantified using associated Magellan™ software by TECAN over a period of 24 h with the kinetic peaks of the positive controls used for comparison.
Keeney J.T., Ren X., Warrier G., Noel T., Powell D.K., Brelsfoard J.M., Sultana R., Saatman K.E., Clair D.K, & Butterfield D.A. (2018). Doxorubicin-induced elevated oxidative stress and neurochemical alterations in brain and cognitive decline: protection by MESNA and insights into mechanisms of chemotherapy-induced cognitive impairment (“chemobrain”). Oncotarget, 9(54), 30324-30339.
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