The MCF-7 human breast adenocarcinoma cell line was purchased from the American Type Culture Collection (Manassas, VA). The MDA-MB-453 human breast cancer cell line was purchased from Thermo Fisher Scientific (Shanghai, China). MCF-7 and MDA-MB-453 cells were cultured in DMEM medium supplemented with 10% FBS, 100 U/ml penicillin, and 0.1 g/ml streptomycin, in a cell incubator with humidified atmosphere (37%, 5% CO2). DMEM medium, FBS, and penicillin-streptomycin for cell culture were purchased from Sigma (Merck Life Science, Shanghai, China). LGR5 overexpression in MCF-7 cells as well as LGR5 knockdown in MDA-MB-453 cells were performed by Genecopoeia (Guangzhou, China). Briefly, for LGR5 overexpression, LGR5 cDNA-carrying pcDNA3.1 vector was transfected into MCF-7 cells, and cells transfected with empty vector were used as wild-type control (WT). For LGR5 knockdown, short hairpin RNA (shRNA) targeting LGR5 mRNA was transfected into MDA-MB-453 cells, and cells transfected with non-targeting shRNA was used as wild-type control (WT). Equal LGR5 mRNA and protein expression in non-transfected cells and transfected control cells were confirmed by RT-qPCR and Western blot (data not shown).
Mcf 7
Manufactured by GeneCopoeia
Sourced in China, United States
MCF-7 is a cell line derived from a human breast adenocarcinoma. It is commonly used in cell biology and cancer research studies.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Dmem medium, by Merck Group (1 mentions)
Mcf 7 human breast adenocarcinoma cell line, by American Type Culture Collection (1 mentions)
Mcf 7 atcc htb 22, by American Type Culture Collection (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Hs578t, by Thermo Fisher Scientific (1 mentions)
2 protocols using mcf 7
1
Modulating LGR5 in Breast Cancer Cells
2
BAALC Regulation in Breast Cancer
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MCF-7 (ATCC HTB-22) and Hs578T (ATCC HTB-126) cells were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA) immediately prior to commencing this study. Cells were maintained in DMEM, supplemented with 15 mM HEPES, 2 mM glutamine, and 10% fetal calf serum (FCS), with additional 0.01mg/ml bovine insulin for Hs578T. All cell culture reagents were purchased from Thermo Fischer Scientific (Mulgrave, VIC, Australia). MCF-7 cells were stably transduced with either empty vector (EV) or full length BAALC (Lv41-BAALC) pseudoviral particles (GeneCopoeia, Rockville, MD, USA), as per manufacturer’s instructions.
Hs578T cells were transiently transfected with siRNA directed against BAALC (or scrambled control sequences) by Lipofectamine 3000 (Thermo Fisher Scientific) as previously described (35 (link)). Pools of three to five target-specific 19-25 nucleotide siRNAs designed to knockdown BAALC expression (and control siRNA sequences) were purchased from Santa Cruz Technology (Dallas, Tx, USA). Cells were then lysed at various times post-transfection or used in functional assays to measure effects on proliferation, migration and invasion, as described below.
Hs578T cells were transiently transfected with siRNA directed against BAALC (or scrambled control sequences) by Lipofectamine 3000 (Thermo Fisher Scientific) as previously described (35 (link)). Pools of three to five target-specific 19-25 nucleotide siRNAs designed to knockdown BAALC expression (and control siRNA sequences) were purchased from Santa Cruz Technology (Dallas, Tx, USA). Cells were then lysed at various times post-transfection or used in functional assays to measure effects on proliferation, migration and invasion, as described below.
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