1 4 butanediol diacrylate b4

Manufactured by Thermo Fisher Scientific

1,4-butanediol diacrylate (B4) is a chemical compound used in various laboratory applications. It functions as a crosslinking agent, which helps to form stable, three-dimensional networks in materials.

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1,4-butanediol (15 citations) 1,4-Butanediol diacrylate (5 citations) 1,4-Butanediol diacrylate (B4), 5-amino-1-pentanol (S5), (2 citations)

5 protocols using 1 4 butanediol diacrylate b4

Polymer Synthesis and Transfection Reagents

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Lipofectamine 2000 (Invitrogen, Carlsbad, CA), Opti-MEM I (Invitrogen), pEGFP-N1 DNA (Elim Biopharmaceuticals, Hayward, CA), pDsRed-Max-N1^{39 (link)} (Addgene DNA plasmid 21718, Cambridge, MA), and cell culture media components were used as received. Monomers used for synthesizing polymers (Scheme 1) were purchased as follows: 1,3-butanediol diacrylate (B3b; Sigma-Aldrich, St. Louis, MO); 1,4-butanediol diacrylate (B4; Alfa Aesar, Ward Hill, MA); 1,5-pentanediol diacrylate (B5; Monomer-Polymer and Dajac Laboratories, Trevose, PA); 3-amino-1-propanol (S3; Alfa Aesar); 4-amino-1-butanol (S4; Alfa Aesar); 5-amino-1-pentanol (S5; Alfa Aesar); 1,3-diaminopropane (E1; Sigma-Aldrich); 1,3-diaminopentane (E3; TCI America, Portland, OR); 2-methyl-1,5-diaminopentane (E4; TCI America); 1,11-diamino-3,6,9-trioxaundecane (E5; TCI America); 2-(3-aminopropylamino)ethanol (E6; Sigma-Aldrich); 1-(3-aminopropyl)-4-methylpiperazine (E7; Alfa Aesar); 1-(3-aminopropyl)pyrrolidine (E8; TCI America); and cystamine dihydrochloride (E10; Alfa Aesar). 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) was purchased from Sigma and used as a 750 nM solution in PBS. Other materials were reagent grade.

Guerrero-Cázares H., Tzeng S.Y., Young N.P., Abutaleb A.O., Quiñones-Hinojosa A, & Green J.J. (2014). Biodegradable Polymeric Nanoparticles Show High Efficacy and Specificity at DNA Delivery to Human Glioblastoma in Vitro and in Vivo. ACS Nano, 8(5), 5141-5153.

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Differentiation of Neural Progenitor Cells

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Homo sapiens neurogenin-2 (Ngn2, pCMV6 vector, SC322602) and achaete-scute complex homolog 1 (Mash1, pCMV6 vector, SC117427) as transfection-ready DNAs were obtained from OriGene Technologies (Rockville, MD). pEGFP-N1 DNA was obtained from Elim Biopharmaceuticals (Hayward, CA). Recombinant fibroblast growth factor-2 (FGF-2) and epidermal growth factor (EGF) were purchased from Peprotech (Rocky Hill, NJ). Accutase was from Innovative Cell Technologies (San Diego, CA). Mouse laminin was obtained from Invitrogen (Carlsbad, CA), and 4′, 6-diamidino-2-phenylindole, dihydrochloride (DAPI) was from Molecular Probes (Eugene, OR). Primary antibodies used in this study are shown in Table S1. Fluorophore-conjugated secondary antibodies were purchased from Jackson ImmunoResearch (West Grove, PA).
Monomers used for synthesizing polymers (Scheme 1) were obtained as follows: 1,4-butanediol diacrylate (B4; Alfa Aesar, Ward Hill, MA), 1,5-pentanediol diacrylate (B5; Monomer-Polymer and Dajac Labs, Trevose, PA), 3-amino-1-propanol (S3; Alfa Aesar), 4-amino-1-butanol (S4; Alfa Aesar), 5-amino-1-pentanol (S5; Alfa Aesar), 2-(3-aminopropylamino) ethanol (E6; Sigma Aldrich, St. Louis, MO), and 1-(3-aminopropyl)-4-methylpiperazine (E7; Alfa Aesar). All other chemical reagents were from Sigma Aldrich (St. Louis, MO).

Li X., Tzeng S.Y., Liu X., Tammia M., Cheng Y.H., Rolfe A., Sun D., Zhang N., Green J.J., Wen X, & Mao H.Q. (2016). Nanoparticle-mediated Transcriptional Modification Enhances Neuronal Differentiation of Human Neural Stem Cells Following Transplantation in Rat Brain. Biomaterials, 84, 157-166.

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Synthesis and Characterization of PBAEs

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PBAEs were synthesized as described previously.19 (link) Briefly, backbone monomers, 1,4-Butanediol diacrylate (B4, Alfa Aesar, Haverhill, MA) or 1,5-Pentanediol diacrylate (B5, Santa Cruz Biotechnology, Dallas, TX), were mixed at a 1.1:1 ratio with sidechain monomers 3-amino-1-propanol (S3, Alfa Aesar, Haverhill, MA), 4-amino-1-butanol (S4, Fisher Scientific, Hampton, NH), or 5-amino-1-pentanol (S5, Alfa Aesar, Haverhill, MA) and allowed to react overnight at 85°C. B-S polymers were then resuspended in tetrahydrofuran (THF) and endcapped with 2-(3-Aminopropylamino)ethanol (E6, Sigma, St. Louis, MO) or 1-(3-Aminopropyl)-4-methylpiperazine (E7, Alfa Aesar, Haverhill, MA) for 2 h at room temperature. Resulting polymers B4-S4-E6 (446), B4-S5-E7 (457), and B5-S3-E6 (536) were ether precipitated, dried, and stored at −20°C on desiccant. To determine the molecular weight and polydispersity index of PBAE 536, ether purified polymers were resuspended in tetrahydrofuran and gel permeation chromatography was performed using polystyrene reference standards and a refractive index detector (Agilent, Santa Clara, CA).

Vaughan H.J., Zamboni C.G., Luly K.M., Li L., Gabrielson K.L., Hassan L.F., Radant N.P., Bhardwaj P., Selaru F.M., Pomper M.G, & Green J.J. (2023). Non-Viral Gene Delivery to Hepatocellular Carcinoma via Intra-Arterial Injection. International Journal of Nanomedicine, 18, 2525-2537.

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Polymer-Mediated Transfection of Stem Cell Regulators

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Homo sapiens SRY (sex determining region Y)-box 2 (Sox2, CMV Promoter, RG200757) and oligodendrocyte transcription factor 2 (Olig2, CMV Promoter, RG208209) as transfection-ready DNAs were obtained from OriGene Technologies (Rockville, MD). pEGFP-N1 DNA was obtained from Elim Biopharmaceuticals (Hayward, CA). Recombinant human fibroblast growth factor-2 (FGF-2) and epidermal growth factor (EGF) were purchased from Peprotech (Rocky Hill, NJ). Poly-l-lysine was obtained from ScienCell Research Laboratories (Carlsbad, CA). 4′,6-Diamidino-2-phenylindole, dihydrochloride (DAPI) was purchased from Molecular Probes (Eugene, OR). Primers and primary antibodies used in this study are shown in Tables S1 and S2,† respectively. Fluorophore-conjugated secondary antibodies were purchased from Jackson ImmunoResearch (West Grove, PA).
Monomers used for synthesizing polymers (Scheme 1) were obtained as follows: 1,4-butanediol diacrylate (B4; Alfa Aesar, Ward Hill, MA), 1,5-pentanediol diacrylate (B5; Monomer-Polymer and Dajac Labs, Trevose, PA), 3-amino-1-propanol (S3; Alfa Aesar), 4-amino-1-butanol (S4; Alfa Aesar), 5-amino-1-pentanol (S5; Alfa Aesar), 2-(3-aminopropylamino) ethanol (E6; Sigma Aldrich, St Louis, MO), and 1-(3-aminopropyl)-4-methylpiperazine (E7; Alfa Aesar). All other chemical reagents were obtained from Sigma Aldrich (St. Louis, MO).

Li X., Kozielski K., Cheng Y.H., Liu H., Zamboni C.G., Green J, & Mao H.Q. (2016). Nanoparticle-mediated conversion of primary human astrocytes into neurons and oligodendrocytes. Biomaterials science, 4(7), 1100-1112.

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Synthesis and Characterization of Acrylate-Based Biomaterials

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1,4-butanediol diacrylate (B4) (Alfa Aesar, Ward Hill, MA), 1,5-pentanediol diacrylate (B5) (Monomer Polymer & Dajac Labs), 3-amino-1-propanol (S3) (Alfa Aesar), 4-amino-1-pentanol (S4) (Alfa Aesar), 5-amino-1-pentanol (S5) (Alfa Aesar), 2-methylpentane-1,5-diamine (E4) (TCI America, Portland, OR), 2-(3-aminopropylamino)ethanol (E6) (Sigma-Aldrich, St. Louis, MO), and 1-(3-aminopropyl)-4-methylpiperazine (E7) (Alfa Aesar) were purchased and used as received. pEGFP-N1 was purchased from Elim Biopharmaceuticals and amplified by Aldevron (Fargo, ND). pfLuc Luciferase-pcDNA3 was a gift from William Kaelin (Addgene plasmid # 18964) and amplified by Aldevron (Fargo, ND). Purelink Genomic DNA Extraction kit (Thermo Fisher Scientific), PowerUp SYBR Green Master Mix (Applied Biosystems, Foster City, CA), Label IT®-Tracker™ Cy™3 and Cy™5 kit (Mirus Bio LLC, Madison, WI) were obtained from commercial vendors and used per manufacturer’s instructions.

Kim J., Vaughan H.J., Zamboni C.G., Sunshine J.C, & Green J.J. (2021). High-throughput evaluation of polymeric nanoparticles for tissue-targeted gene expression using barcoded plasmid DNA. Journal of controlled release : official journal of the Controlled Release Society, 337, 105-116.

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