Experimental animals were imaged using microPET/SPECT/CT system Albira (Bruker, Billerica, Massachusetts, USA). Mice were r.o. injected with the radiolabeled tracer at a dose of 5–10 MBq (corresponding to 1–2 μg of peptide for animals injected with 68Ga-peptides) per animal. Anesthetized animals were placed in a prone position in the Albira system before the start of imaging. Static PET/CT images were acquired over 30 min interval starting 30 and 90 minutes after injection. A 10-min PET scan (axial FOV 148 mm) was performed, followed by a double CT scan (axial FOV 65 mm, 45 kVp, 400 μA, at 400 projections). Scans were reconstructed with the Albira software (Bruker Biospin Corporation, Woodbridge, CT, USA) using the maximum likelihood expectation maximization (MLEM) and filtered back-projection (FBP) algorithms. The PMOD software (PMOD Technologies, Zurich, Switzerland) was used for the image analysis and tracer uptake quantification. Three-dimensional volume rendered images were created in VolView software (Kitware, Clifton Park, NY, USA). The data obtained by PET scan were quantified in PMOD via manually selected volumes of interest (VOIs) and expressed as standardized uptake values (SUV). Using SUVs for the tumor and brain regions the tumor to brain ratios were calculated.
Albira
Manufactured by Bruker
Sourced in United States
The Albira is a preclinical imaging system from Bruker designed for small animal imaging. It offers multimodal capabilities, including positron emission tomography (PET), single-photon emission computed tomography (SPECT), and computed tomography (CT). The Albira provides high-resolution imaging for research applications.
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Lab products found in correlation
6 protocols using albira
1
Multimodal Imaging of Radiolabeled Tracers
2
Small Animal SPECT/CT Imaging of Radiolabeled HAP NPs
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The single photon emission computed tomography/computed tomography (SPECT/CT) scans were performed with a small animal PET/SPECT/CT imaging system (Albira, Bruker Biospin Corporation, Woodbridge, CT, USA). During the scans, the mice were anesthetized with 2% isoflurane. Static whole-body SPECT scans of 30 min duration were performed at 1, 3, 6 and 24 h after retroorbital injection of 99mTc-HEDP-labeled HAP NPs (~1.3 mg NPs; 40–60 MBq/mouse). The SPECT scans were followed by a double CT of 20 min (axial FOV 2 × 65 mm, 45 kVp, 400μA, at 400 projections). Reconstruction of the acquired data was performed with the Albira software (Bruker Biospin Corporation, Woodbridge, CT, USA) using the ordered subset expectation maximization and filtered backprojection (FBP) algorithms. All images were prepared using PMOD software, v. 3.307 (PMOD Technologies Ltd., Zurich, Switzerland).
3
Quantifying Skeletal Muscle FDG Uptake in ALS Mice
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Sample size of mice submitted to micro-PET imaging was defined based on the hypothesis of a 40% increase of skeletal muscle FDG uptake induced by ALS, coupled with an expected variation coefficient of tracer retention in this same tissue approaching 16%. These features were entered into a freely available calculator (http://www.rad.jhmi.edu/jeng/javarad/samplesize/#references ) that estimated sample size to five mice per group as proposed by Eng et al. [18 (link)]. All animals were fasted for 6 h, weighted, and anesthetized with intra-peritoneal ketamine (100 mg/Kg) and xylazine (10 mg/kg). Serum glucose level was tested and FDG (3–4 MBq) was injected through a tail vein. Forty minutes later, mice underwent a 10-min static acquisition in a dedicated micro-PET system (Albira, Bruker, USA) whose dual ring configuration allows the acquisition of the whole mouse body. After its completion, mice were immediately euthanized by cervical dislocation.
4
In Vivo Imaging with FDG-PET in Mice
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In vivo imaging was performed according to our validated procedure [14 (link)]. Anesthesia was induced by intraperitoneal administration of ketamine (100 mg/kg) and xylazine (10 mg/kg). Capillary glucose level and body weight were measured, and mice were positioned on the bed of a dedicated micro-PET system (Albira, Bruker Inc., USA). A dose of 3–4 MBq of FDG was then injected through a tail vein, soon after the start of a list mode acquisition lasting 50 min.
5
Standardization of Preclinical PET/CT Imaging
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This multicenter study involved 5 sites for a total of 5 different commercial preclinical PET/CT scanners (Bruker Albira, Mediso nanoPET/CT, Sedecal Super Argus, Siemens Inveon and Trifoil LabPET/CT), arbitrarily labeled 1 to 5. First, routine/default (hereon referred to as default) PET and CT protocols were evaluated for image quality and quantification biases using seven commercially available preclinical microPET and microCT phantoms (Supplemental Table 2, labeled A–G). Default protocols were set either by the vendor or by the site for their routine use of imaging small animals. Each CT default protocol was also assessed based on measured absorbed ionizing radiation. Second, several different PET reconstruction methods were quantitatively analyzed for standardization. Third, standardized CT protocols were determined from the least Hounsfield units (HU) biases between all imaging data sets. Numerical criteria for biases were based on the parameters in Table 1 . Results were then evaluated in the same manner as the default protocols on each scanner. All PET and CT imaging data sets (default and standard) per scanner per phantom and dose measurements were acquired as n = 3 for the analysis. No rodents were used in this study, only dedicated PET and CT phantoms. The lead author visited each site multiple times for the image acquisitions and carried out all the data analysis.
6
Preclinical PET Imaging of Infection
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For this study, 5.2 MBq of [68Ga]Ga-DOTA-UBI29-41 was administered intravenously through the caudal vein of the mice with infectious process. Images were obtained using a microPET/SPECT/CT Albira instrument (Bruker Biospin Corporation, Woodbridge, CT, USA) with the animals under anesthesia with isoflurane at 2% in O2. The images were registered in dynamic mode with 90 frames, for 1 h of acquisition beginning at the administration time. Computed Tomography anatomic images were obtained after PET recordings: a field of view of 80 mm, 35 kV, 400 μA and 400 projections. PET/CT images were reconstructed using the Albira software, version 5.6 (Bruker Biospin Corporation, CT, USA) and processed and evaluated using PMOD software, version 3.1 (PMOD Technologies, Zurick, Switzerland).
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